@ARTICLE{Zhou_Z.-H._17_2020,
 author={Zhou, Z.-H. and Gu, C.-W. and Li, J. and Huang, X.-Y. and Deng, J.-Q. and Shen, L.-H. and Cao, S.-Z. and Deng, J.-L. and Zuo, Z.-C. and Wang, Y. and Ma, X.-P. and Ren, Z.-H. and Yu, S.-M.},
 volume={vol. 23},
 number={No 2},
 journal={Polish Journal of Veterinary Sciences},
 pages={235-245},
 howpublished={online},
 year={2020},
 publisher={Polish Academy of Sciences Committee of Veterinary Sciences},
 publisher={University of Warmia and Mazury in Olsztyn},
 abstract={Emerging researches in humans, pigs and mice, highlighted that estrogen plays a pivotal role in self-renewal and differentiation of bone marrow mesenchymal stem cells (BMSCs). The present study aimed at evaluating effects of 17 beta-estradiol (E2) on proliferation and apoptosis of canine-derived bone marrow mesenchymal stem cells (cBMSCs) in vitro. The results showed that E2 supplementation at the concentration of 10-11 M promoted the proliferation of cBMSCs by CCK-8 assay and RT-qPCR analysis for the proliferation-related genes, with proliferating cell nuclear antigen (PCNA), cyclin-D1 (CCND1) being up-regulated and cyclin-dependent kinase inhibitor 1B (CDKN1B) being down-regulated. Contrarily, analysis of fluorescence-activated cell sorting (FACS) and RT-qPCR demonstrated that E2 supplementation above 10-11 M had inhibitory effects on the proliferation of cBMSCs and induced apoptosis. Intriguingly, cBMSCs still possessed the capability to differentiate into osteoblasts and adipocytes with 10-11 M E2 addition. Taken together, this study determined the optimal culture condition of cBMSCs in vitro, and has important implications for further understanding the regulatory effect of E2 on the self-renewal of cBMSCs, which are helpful for the clinical application of BMSCs.},
 type={Article},
 title={17 beta-estradiol affects proliferation and apoptosis of canine bone marrow mesenchymal stem cells in vitro},
 URL={http://journals.pan.pl/Content/116768/PDF/10.pdf},
 doi={10.24425/pjvs.2020.133638},
 keywords={apoptosis, bone marrow mesenchymal stem cells (BMSCs), canine, proliferation, 17 beta-estradiol (E2)},
}