@ARTICLE{Mozel_S._Immunohistochemical_2020,
 author={Mozel, S. and Szymańczyk, S. and Krzysiak, M. and Puzio, I. and Zacharko-Siembida, A. and Arciszewski, M.B.},
 volume={vol. 23},
 number={No 3},
 journal={Polish Journal of Veterinary Sciences},
 pages={333-340},
 howpublished={online},
 year={2020},
 publisher={Polish Academy of Sciences Committee of Veterinary Sciences},
 publisher={University of Warmia and Mazury in Olsztyn},
 abstract={Previous morphological studies of mammalian pancreatic islets have been performed mainly in domestic and laboratory animals. Therefore, the present immunohistochemical investigation was conducted in a wild species, the European bison, using antibodies against glucagon-like peptide-1 (GLP1), glucagon, insulin and somatostatin. Morphological analyses revealed that the mean area of the endocrine pancreas constituted 2.1±0.1% of the whole area of the pancreas, while the mean area of a single pancreatic islet was 13301.5±686.5 µm2. Glucagon-immunoreac- tive cells accounted for 22.4±1.1% and occupied 19.4±0.4% of the average islet area. As many as 14.3±1.4% of pancreatic islet cells were shown to express GLP1, which constituted 12.6±0.8% of the mean area of the islet. Insulin expression was confirmed in 67.6±0.7% of pancreatic islet cells, which represented 62.3±4.9% of the mean total area of the pancreatic islet. As many as 8.5±1.3% of cells stained for somatostatin. The somatostatin-immunoreactive cell area was 4.9±0.3% of the mean pancreatic islet area. In summary, we have determined in detail for the first time the morphometry and islet composition of the European bison pancreas. The distri- bution patterns of immunoreactivities to the substances studied in the European bison show many similarities to those described in other ruminant species.},
 type={Article},
 title={Immunohistochemical study on the expression of biologically active substances in the endocrine pancreas of the European bison},
 URL={http://journals.pan.pl/Content/116780/PDF/2.pdf},
 doi={10.24425/pjvs.2020.133650},
 keywords={glucagon-like peptide-1, glucagon, insulin, somatostatin, immunohistochemistry, pancreas, European bison},
}