@ARTICLE{Czyczyło-Mysza_Ilona_Indirect_2012, author={Czyczyło-Mysza, Ilona and Skrzypek, Edyta and Marcińska, Izabela}, volume={vol. 54}, number={No 2}, journal={Acta Biologica Cracoviensia s. Botanica}, howpublished={online}, year={2012}, publisher={Biological Commission of the Polish Academy of Sciences – Cracow Branch}, abstract={Using four Polish Vicia faba L. minor cultivars (Bronto, Dino, Tibo, Nadwiślański) we obtained callus from epicotyl fragments collected from 7- and 14-day-old seedlings and from cotyledonary nodes of immature seeds. Callus induction efficiency varied from 81% to 97% depending on the origin of the explant. Shoots regenerated only from the cotyledonary nodes of all tested cultivars. On average, 50% of the explants grown on MS medium containing 1.0 mg dm-3 NAA, 0.5 mg dm-3 BAP, 0.25 mg dm-3 GA3, 1.0 g dm-3 casein hydrolysate, 750 mg dm-3 inositol, 3% sucrose and 0.4% agar were able to regenerate shoots. The number of calluses regenerating shoots was highest from explants collected from fruiting nodes 6 to 9. Decapitation of donor plants increased the percentage of calluses regenerating shoots. On half-strength MS medium with 2 mg dm-3 NAA and on 1/2 MS alone, 11% of the shoots rooted; on 1/2 MS with 1 g dm-3 AC, 8.0% rooted. The regenerants were transferred to Perlite with Hoagland medium and acclimated. Ten percent of the regenerated plants survived the acclimation process, flowered and produced seeds.}, type={Artykuły / Articles}, title={Indirect Organogenesis of Faba Bean (Vicia Faba L. Minor)}, URL={http://journals.pan.pl/Content/81776/PDF/v10182-012-0026-7.pdf}, doi={10.2478/v10182-012-0026-7}, keywords={callus, immature seed, organogenesis, in vitro culture, Vicia faba L. minor}, }