@ARTICLE{Żabicki_Piotr_Arabidopsis_2013,
 author={Żabicki, Piotr and Kuta, Elżbieta and Tuleja, Monika and Rataj, Katarzyna and Malec, Przemysław},
 volume={vol. 55},
 number={No 1},
 journal={Acta Biologica Cracoviensia s. Botanica},
 howpublished={online},
 year={2013},
 publisher={Biological Commission of the Polish Academy of Sciences – Cracow Branch},
 abstract={The Arabidopsis CDKG;2 gene encodes a putative cyclin-dependent Ser/Thr protein kinase of unknown biological function. This gene shows structural similarity to animal and human cyclin-dependent (PITSLRE) kinases. This study used the homozygous knockout cdkg;2 mutant based on T-DNA insertional line SALK_090262 to study the effect of mutation of the CDKG;2 gene on explant response and in vitro plant regeneration. For callus induction and proliferation, hypocotyls and cotyledons of 3-day-old seedlings of cdkg;2 and A. thaliana ecotype Col-0 were cultured on solid MS medium supplemented with 2,4-D (2 mg l-1). Organogenesis was induced after callus transfer on MS + TDZ (0.5 mg l-1). The initiation time of callus and shoot induction differed between the mutant and control cultures. Shoot regeneration after callus transfer on MS + TDZ was delayed in cdkg;2 (31 days versus 7 days in Col- 0). Shoots formed on callus derived from Col-0 hypocotyls but not on cotyledon-derived callus; in cdkg;2, shoots developed on both callus types. Mutant shoots did not form roots, regenerants were dwarfed, and inflorescences had small bud-like flowers with a reduced corolla and generative organs. Abnormalities observed during cdkg;2 organogenesis suggest a role of CDKG;2 as a regulator of adventitious root initiation},
 type={Artykuły / Articles},
 title={Arabidopsis Cyclin-Dependent Kinase Gene CDKG;2 is Involved in Organogenic Responses Induced in Vitro},
 URL={http://journals.pan.pl/Content/81783/PDF/05_zabicki.pdf},
 doi={10.2478/abcsb-2013-0003},
 keywords={Arabidopsis thaliana cyclin-dependent kinases, CDKG, 2 gene, callus formation, organogenesis},
}