TY - JOUR N2 - In vitro embryogenic callus is a critical factor for genetic transformation of rice, especially for indica varieties. In this study, we investigated the relationship between polyamines, including putrescine (Put), spermidine (Spd) and spermine (Spm), and callus browning, and we studied the effect of exogenous Put on callus regeneration and on the content of endogenous polyamines. In addition, the expression levels of arginine decarboxylase gene (Adcl) and S-adenosylmethionine decarboxylase gene (Samdc) in embryogenic callus were studied by quantitative Real-time PCR analysis. The results showed that the contents of endogenous Put and Spd in the browning callus were significantly lower than those in normal callus. Exogenous Put could effectively improve the growing state of callus of indica rice and enhance the development of embryogenic callus. The content of endogenous polyamines in embryogenic callus, especially Spd and Spm, was increased after addition of exogenous Put. Additionally, exogenous Put also had an obvious impact on the expression levels of Adcl but partial effect on the expression levels of Samdc gene. This study could increase the knowledge of both embryogenic callus induction and polyamine catabolism in callus in indica rice. L1 - http://journals.pan.pl/Content/100901/PDF/ABC%20Bot.%201-17%209-Y.Tan%20i%20in.pdf L2 - http://journals.pan.pl/Content/100901 PY - 2017 IS - No 1 DO - 10.1515/abcsb-2017-0001 KW - browning KW - callus KW - gene expression KW - indica rice KW - polyamine A1 - Tan, YP A1 - Hu, W A1 - Xu, X A1 - Zhou, J A1 - Wang, CT A1 - Liu, XQ A1 - Cheng, G PB - Biological Commission of the Polish Academy of Sciences – Cracow Branch VL - vol. 59 DA - 2017.06.30 T1 - Polyamine plays a role in subculture growth of in vitro callus of indica rice UR - http://journals.pan.pl/dlibra/publication/edition/100901 T2 - Acta Biologica Cracoviensia s. Botanica ER -