TY - JOUR N2 - Sapelovirus A (SV-A) is a positive-sense single-stranded RNA virus which is associated with acute diarrhea, pneumonia and reproductive disorders. The virus capsid is composed of four proteins, and the functions of the structural proteins are unclear. In this study, we expressed SV-A structural protein VP1 and studied its antigenicity and immunogenicity. SDS-PAGE analysis revealed that the target gene was expressed at high levels at 0.6 mM concentration of IPTG for 24 h. The mouse polyclonal antibody against SV-A VP1 protein was produced and reached a high antiserum titer (1: 2,048,000). Immunized mice sera with the recombinant SV-A VP1 protein showed specific recognition of purified VP1 protein by western blot assay and could recognize native SV-A VP1 protein in PK-15 cells infected with SV-A by indirect immunofluorescence assay. The successfully purified recombinant protein was able to preserve its antigenic determinants and the generated mouse anti-SV-A VP1 antibodies could recognize native SV-A, which may have the potential to be used to detect SV-A infection in pigs. L1 - http://journals.pan.pl/Content/108446/PDF/20.pdf L2 - http://journals.pan.pl/Content/108446 PY - 2018 IS - No 3 EP - 573–579 DO - 10.24425/124292 KW - sapelovirus A KW - prokaryotic expression KW - purification A1 - Zhao, T.T. A1 - Cui, L. A1 - Chen, L. A1 - Li, J.J. A1 - Liang, Q.L. A1 - Wu, P.J. A1 - Yu, X.Q. A1 - Zhang, Z.H. A1 - Hua, X.G. PB - Polish Academy of Sciences Committee of Veterinary Sciences PB - University of Warmia and Mazury in Olsztyn VL - vol. 21 DA - 2018.11.20 T1 - Optimal expression and purification of sapelovirus A structural protein VP1, and its immunogenicity in mice SP - 573–579 UR - http://journals.pan.pl/dlibra/publication/edition/108446 T2 - Polish Journal of Veterinary Sciences ER -