TY - JOUR N2 - Polychlorinated biphenyls (PCBs), dichlorodiphenyltrichloroethane (DDT) and its metabolite, dichlorodiphenyldichloroethylene (DDE) can disturb the secretory function of the ovary and both contractions and secretory function of the uterus during the estrus cycle and pregnancy. Additionally, PCBs can pass through the placental barrier into allantoic and amniotic fluid. The presence of PCBs in these fluids is associated with higher frequency of spontaneous abortions and premature births in humans and animals. Therefore, the effect of PCBs, DDT and DDE on the connexins (Cx26, Cx32 and Cx43) and keratin 8 (KRT8) expression in bovine placentomes was investigated. The placentome slices from the second trimester of pregnancy were incubated with PCB153, 126, 77, DDT and DDE (each at doses of 1, 10 or 100 ng/ml) for 48 h. Then, the slices were stained using immunohistochemistry. The density of Cxs staining was measured with Axio- Vision Rel. 4.8 software in fetal-maternal connections and binuclear cells (BNC). None of the tested xenobiotics (XBs) affected the localization of Cxs and KRT8 in the fetal-maternal connection area, but the XBs affected the density of Cxs in fetal-maternal connections and binuclear cells (BNCs). Depend on the doses, in fetal-maternal connections all used PCBs changed the protein expression of different Cxs, while in BNCs, all tested XBs except DDT increased the expression of Cxs. None of investigated XBs affected on KRT8 expression. In summary, used XBs affect the expression of Cxs and change the quantitative relationships between them. Therefore, XBs can unfavorably influence function of the utero-placental barrier in cows. L1 - http://journals.pan.pl/Content/108465/PDF/09.pdf L2 - http://journals.pan.pl/Content/108465 PY - 2018 IS - No 4 EP - 721–729 DO - 10.24425/124311 KW - connexins expression KW - KRT8 KW - chlorinated xenobiotics KW - cotyledons KW - cow A1 - Wojciechowska, A. A1 - Młynarczuk, J. A1 - Kotwica, J. PB - Polish Academy of Sciences Committee of Veterinary Sciences PB - University of Warmia and Mazury in Olsztyn VL - vol. 21 DA - 2018.12.20 T1 - The protein expression disorders of connexins (Cx26, Cx32 and Cx43) and keratin 8 in bovine placenta under the influence of DDT, DDE and PCBs SP - 721–729 UR - http://journals.pan.pl/dlibra/publication/edition/108465 T2 - Polish Journal of Veterinary Sciences ER -