TY - JOUR N2 - Objective: The aim of this study was to verify if the exposure to the pulsed electromagnetic field (PEMF) infl uenced the release of proinfl ammatory cytokines from adipose-derived stem cells (ADSCs) of normal and overweight rats of various age and sex. Moreover, we compared body temperatures of normal-weight and overweight rats. Methods: ADSCs of Wistar rats were isolated from the subcutaneous area in females and paratesticular region in males, cultured and exposed to PEMF (7 Hz, 30 mT). Concentrations of proinflammatory cytokines were determined in rat sera and supernatant from ADSCs cultures exposed and non-exposed to PEMF. Body temperature (BT) was measured twice a week, using an infrared and rectal thermometer. Results: Irrespective of age and sex, animals maintained on low-fat (LF) diet had higher BT than those grown on high-fat (HF) diet. Exposure to PEMF reduced the release of TNF-α and enhanced the production of IL-6 in ADSCs cultures from female pups maintained on LF diet. In contrast, a decrease in IL-6 level was observed in PEMF-exposed ADSCs cultures from female pups grown on HF diet. A similar phenomenon, i.e. a post-exposure increase in IL-6 level was also observed in male pups fed with the LF diet. In the case of ADSCs cultures from adult rats maintained on an HF diet, either males or females, PEMF exposure contributed to a dramatic increase in TNF-α production. Conclusion: Our findings suggest that PEMF exposure may affect the production of proinflammatory cytokines in ADSCs cultures. The intergroup diff erences in BT may result from the presence of an underlying inflammation in obese rats. L1 - http://journals.pan.pl/Content/109685/PDF/FMC%204-18%203-Baranowska.pdf L2 - http://journals.pan.pl/Content/109685 PY - 2018 IS - No 4 EP - 34 DO - 10.24425/fmc.2018.125701 KW - obesity KW - PEMF KW - ADSCs KW - proinflammatory cytokine KW - temperature A1 - Baranowska, Agnieszka A1 - Skowron, Beata A1 - Gil, Krzysztof A1 - Kaszuba-Zwoińska, Jolanta PB - Oddział PAN w Krakowie; Uniwersytet Jagielloński – Collegium Medicum VL - vol. 58 DA - 2018.12.15 T1 - Effect of the pulsed electromagnetic field on the release of inflammatory mediators from adipose-derived stem cells (ADSCs) in rats SP - 21 UR - http://journals.pan.pl/dlibra/publication/edition/109685 T2 - Folia Medica Cracoviensia ER -