TY - JOUR N2 - The objectives of this study were to determine the genetic relatedness, antibiogram and virulence factors of Staphylococcus aureus (S. aureus) isolated from bovine mastitis, associated farm workers, dairy cow farm veterinarians (private veterinarians), veterinary students, and non-veterinary university students. A total of 84 S. aureus isolates (27 from mastitis, 11 from farm workers, 9 from private veterinarians, 22 from veterinary students, and 16 from non-veterinary university students) were used to determine antimicrobial sensitivity patterns using disk diffusion test, virulence factors using PCR technique and phylogenic analysis using pulsed field gel electrophoresis. All S. aureus isolates were resistant to 2 or more commonly used antibiotics. All isolates from mastitis, farm workers, and veterinary students carried the genes encoding coagulase and thermonuclease factors while isolates from non-veterinary university students carried the genes encoding coagulase, clumping, and thermonuclease factors. The mecA gene was detected in 22.2%, 81.8%, 100%, 95.5% and 100% of isolates from mastitis, farm workers, private veterinarians, veterinary students, and non-veterinary university students, respectively. In the phylogenic analysis, 10 (45.5%), 6 (66.7%) and 8 (72.7%) isolates from veterinary students, private veterinarians and farm workers, respectively were more than 80% similar to isolates from mastitis. Results of this study indicate that S. aureus isolates from mastitis milk and those from related dairy cow personnel and veterinarians share similar antimicrobial sensitivity patterns and virulence factors, therefore a common source of bacteria may exist. Furthermore, possible transmission of S. aureus between cows and cow-related personnel and vice versa could also exist. L1 - http://journals.pan.pl/Content/115997/PDF/16.pdf L2 - http://journals.pan.pl/Content/115997 PY - 2020 IS - No 1 EP - 141 DO - 10.24425/pjvs.2020.132757 KW - intramammary infection KW - dairy cows KW - multi-drug resistant S. aureus KW - antimicrobial resistance A1 - Alekish, M.O. A1 - Bani Ismail, Z. A1 - Gharaibeh, M. A1 - Abu-Qatous, L. PB - Polish Academy of Sciences Committee of Veterinary Sciences PB - University of Warmia and Mazury in Olsztyn VL - vol. 23 DA - 2020.03.23 T1 - Genetic relatedness, antibiogram and virulence factors of Staphylococcus aureus isolated from bovine mastitis and related human contacts SP - 133 UR - http://journals.pan.pl/dlibra/publication/edition/115997 T2 - Polish Journal of Veterinary Sciences ER -