TY - JOUR N2 - An HPLC-DAD method was developed for the determination of formaldehyde in animal feed and silage. The method is based on the determination of the product of chemical reaction between formaldehyde and 2,4-dinitrophenylhydrazine. A 3 g of feed or silage were extracted with Milli-Q water with phosphoric acid and next formaldehyde was derivative with the use 2,4-dinitrophenyl- hydrazine in acetronitrile solution. The extract was purified with 0.45 µm syringe filters and separeted on Zorbax Eclipse XDB C18 column and detection was carried out at 360 nm. Formal- dehyde was eluted with a mobile phase consisting of acetonitrile/water in isocratic elution. This method provided average recoveries of 90.6% to 102.2%, with CVs of 2.6% to 6.4% for feed and from 91.3% to 108.7% with CVs of 1.1% to 4.1% for silage in the ranged of 50 to 1000 mg/kg feeds and silage. The LOD and LOQ for formaldehyde in feed and silage ranged from 1.6 to 2.6 and 2.7 to 5.7 mg/kg, respectively. The methodology was applied for the analysis of feed and silage samples collected from poultry, pigs and cows farms. L1 - http://journals.pan.pl/Content/117737/PDF/10.pdf L2 - http://journals.pan.pl/Content/117737 PY - 2020 IS - No 4 EP - 561 DO - 10.24425/pjvs.2020.134705 KW - formaldehyde KW - feed KW - silage KW - 2,4,-dinitrophenylhydrazine KW - validation KW - HPLC-DA A1 - Patyra, E. A1 - Kwiatek, K. PB - Polish Academy of Sciences Committee of Veterinary Sciences PB - University of Warmia and Mazury in Olsztyn VL - vol. 23 DA - 2020.12.28 T1 - In-house validation method for quantification of formaldehyde in silage and feedingstuffs with the use HPLC-DAD technique SP - 553 UR - http://journals.pan.pl/dlibra/publication/edition/117737 T2 - Polish Journal of Veterinary Sciences ER -