TY - JOUR N2 - We investigated direct and indirect formation of somatic embryogenesis in Brassica oleracea var. botrytis (cauliflower), a very important vegetable crop worldwide. Direct somatic embryogenesis, which is rather rare, was achieved in culture of 2-week-old hypocotyl explants of Brassica oleracea var. botrytis on MS medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5; 1.0; and 1.5 mg/l kinetin. Initial induction of embryogenic callus was achieved on MS supplemented with very low concentrations of 2,4-D (0.05 mg/l and 0.1 mg/l). Indirect somatic embryogenesis from leaf sections was obtained on MS supplemented with 0.05 or 0.1 mg/l 2,4-D. We examined various stages of somatic embryos (globular, heart, torpedo, cotyledonary). More embryos per explant were produced through the indirect pathway (23-25) than through the direct pathway (14-19). The number of embryos produced was high. There is a potential for recurrent, repeated or secondary somatic embryogenesis, possibly an unlimited source for mass propagation and ideal for synthetic seed production in this species. Plant regeneration was achieved on half-strength MS medium without any hormones. L1 - http://journals.pan.pl/Content/81717/PDF/abc53_1_04.pdf L2 - http://journals.pan.pl/Content/81717 PY - 2011 IS - No 1 DO - 10.2478/v10182-011-0004-5 KW - somatic embryogenesis KW - cauliflower KW - Brassica oleracea var. botrytis KW - embryogenic callus KW - tissue culture A1 - Siong, Poon Kok A1 - Taha, Rosna Mat A1 - Rahiman, Fatimah Abdul PB - Biological Commission of the Polish Academy of Sciences – Cracow Branch VL - vol. 53 DA - 2011 T1 - Somatic Embryogenesis and Plant Regeneration from Hypocotyl and Leaf Explants of Brassica Oleracea var. Botrytis (Cauliflower) UR - http://journals.pan.pl/dlibra/publication/edition/81717 T2 - Acta Biologica Cracoviensia s. Botanica ER -