TY - JOUR N2 - The Arabidopsis CDKG;2 gene encodes a putative cyclin-dependent Ser/Thr protein kinase of unknown biological function. This gene shows structural similarity to animal and human cyclin-dependent (PITSLRE) kinases. This study used the homozygous knockout cdkg;2 mutant based on T-DNA insertional line SALK_090262 to study the effect of mutation of the CDKG;2 gene on explant response and in vitro plant regeneration. For callus induction and proliferation, hypocotyls and cotyledons of 3-day-old seedlings of cdkg;2 and A. thaliana ecotype Col-0 were cultured on solid MS medium supplemented with 2,4-D (2 mg l-1). Organogenesis was induced after callus transfer on MS + TDZ (0.5 mg l-1). The initiation time of callus and shoot induction differed between the mutant and control cultures. Shoot regeneration after callus transfer on MS + TDZ was delayed in cdkg;2 (31 days versus 7 days in Col- 0). Shoots formed on callus derived from Col-0 hypocotyls but not on cotyledon-derived callus; in cdkg;2, shoots developed on both callus types. Mutant shoots did not form roots, regenerants were dwarfed, and inflorescences had small bud-like flowers with a reduced corolla and generative organs. Abnormalities observed during cdkg;2 organogenesis suggest a role of CDKG;2 as a regulator of adventitious root initiation L1 - http://journals.pan.pl/Content/81783/PDF/05_zabicki.pdf L2 - http://journals.pan.pl/Content/81783 PY - 2013 IS - No 1 DO - 10.2478/abcsb-2013-0003 KW - Arabidopsis thaliana cyclin-dependent kinases KW - CDKG KW - 2 gene KW - callus formation KW - organogenesis A1 - Żabicki, Piotr A1 - Kuta, Elżbieta A1 - Tuleja, Monika A1 - Rataj, Katarzyna A1 - Malec, Przemysław PB - Biological Commission of the Polish Academy of Sciences – Cracow Branch VL - vol. 55 DA - 2013 T1 - Arabidopsis Cyclin-Dependent Kinase Gene CDKG;2 is Involved in Organogenic Responses Induced in Vitro UR - http://journals.pan.pl/dlibra/publication/edition/81783 T2 - Acta Biologica Cracoviensia s. Botanica ER -