TitleA simple method for extracting DNA from rhododendron plants infected with Phytophthora spp. for use in PCR
Journal titleJournal of Plant Protection Research
Divisions of PASNauki Biologiczne i Rolnicze
4 issues per year.
The online version of Journal of Plant Protection Research (JPPR) is the original one.
Rejection rate – over 70%.
Journal of Plant Protection Research is an international peer-reviewed journal that publishes original papers, rapid communications, reviews, covering all areas of plant protection. Subjects include phytopathological virology, bacteriology, mycology and applied nematology and entomology as well as topics on protecting crop plants and stocks of crop products against diseases, viruses, weeds etc.
The Journal is published by Institute of Plant Protection – National Research Institute and Committee on Agronomic Sciences of the Polish Academy of Sciences. By 1997 under the title Prace Naukowe Instytutu Ochrony Roślin and Annals of Agricultural Sciences - Series E - Plant Protection).
JPPR publishes original research papers, rapid communications, critical reviews, and book reviews covering all areas of modern plant protection. Subjects include phytopathological virology, bacteriology, mycology and applied nematology and entomology as well as topics on protecting crop plants and stocks of crop products against diseases, viruses, weeds etc. We publish papers which use an interdisciplinary approach showing how different control strategies can be integrated into pest management programmes, which cover high and low input agricultural systems worldwide, within the framework of ecologically sound and economically responsible land cultivation.
Relevant topics include: advanced methods of diagnostic, and computer-assisted diagnostic plant research and new findings, biological methods of plant protection, selective chemical methods of plant protection, the effects of plant-protecting agents and their toxicology, methods to induce and utilize crop resistance, application techniques, and economic aspects of plant protection.
Journal of Plant Protection Research is also available on:
The Journal does not have article processing charges (APCs) nor article submission charges.
Journal of Plant Protection Research is published based on the Open Access model.
JPPR is a member of CrossRef – the citation-linking backbone for online publications.
JPPR is indexed/abstracted in:
AGRICOLA, AGRIS, AGRO, BIOSIS Preview, CABI, Chemical Abstracts Services (CAS), DOAJ (Directory of Open Access Journals), EBSCO, FSTA - Food Science & Technology Abstracts, Geobase, Google Scholar, Index Copernicus, Japan Science and Technology Agency (JST), J-Gate, JournalGuide, JournalTOCs, KESLI-NDSL (Korean National Discovery for Science Leaders), Microsoft Academic, Naviga (Softweco), Paperbase, Pirabase, POL-index, Polish Scientific Journals Contents, Polymer Library, Primo Central (ExLibris), ProQuest (relevant databases), Publons, ReadCube, SCOPUS, Sherpa/RoMEO, Summon (Serials Solutions/ProQuest), TDNet, Ulrich's Periodicals Directory/ulrichsweb, WanFang Data, WorldCat (OCLC), Zoological Record.
It is also ranked in SCImago Journal & Country Rank in three categories: Agronomy and Crop Science – Q2, Plant Science – Q2, and Soil Science – Q2 (June 2017).
PublisherCommittee of Plant Protection PAS ; Institute of Plant Protection – National Research Institute
IdentifiereISSN 1899–007X ; ISSN 1427–4345
ReferencesIppolito (2004), Realtime detection of Phytophthora nicotianae and P citrophthora in citrus roots and soil, European Journal of Plant Pathology, 110, 833, doi.org/10.1007/s10658-004-5571-9 ; Khanuja (1999), Rapid isolation of DNA from dry and fresh samples of plants producing large amounts of secondary metabolites and essential oils, Plant Molecular Biology Reporter, 17, 74, doi.org/10.1023/A:1007528101452 ; Wiejacha (2002), Optimization of DNA isolation procedure as the first step in identification of Phytophthora spp Bulletin of the Polish Academy of Sciences Biological www qiagen com Accessed, Sciences, 50, 165. ; Bashalkhanov (2008), Protocol A high - throughput DNA extraction system suitable for conifers, Plant Methods, 4. ; Aljanabi (1997), Universal and rapid salt - extraction of high quality genomic DNA for PCR - based techniques, Nucleic Acids Research, 25, 4692, doi.org/10.1093/nar/25.22.4692 ; Michiels (2003), den Ende Extraction of high - quality genomic DNA from latex - containing plants, Analytical Biochemistry, 315. ; Hu (2003), Target region amplification polymorphism : A novel marker technique for plant genotyping, Plant Molecular Biology Reporter, 21, 289, doi.org/10.1007/BF02772804 ; Sahu (2012), DNA extraction protocol for plants with high levels of secondary metabolites and polysaccharides without using liquid nitrogen and phenol International Scholarly Research Network , International Scholarly Research Notices ( ISRN, Molecular Biology, doi.org/10.5402/2012/205049 ; Demeke (2010), Influence of DNA extraction methods , PCR inhibitors and quantification methods on realtime PCR assay of biotechnology - derived traits, Analytical and Bioanalytical Chemistry, 396. ; Weishing (1994), DNA isolation and purification In : DNA Fingerprinting in Plants and Fungi eds CRC Press Florida, USA, 44. ; Böhm (1999), Real - time quantitative PCR : DNA determination in isolated spores of the mycorrhizal fungus Glomus mosseae and monitoring of Phytophthora infestans and Phytophthora citricola in their respective host plants, Journal of Phytopathology, 7, 147. ; OEPP (2006), Diagnostic protocol for Phytophthora ramorum OEPP, EPPO Bulletin, 36, 145. ; Wiejacha (2001), Molecular markers in detection of distant hybrids in Lilium, Acta Horticulturae, 546. ; Porebski (1997), Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components, Plant Molecular Biology Reporter, 15, 8, doi.org/10.1007/BF02772108 ; Doyle (1987), A rapid DNA isolation procedure for small quantities of fresh leaf tissues, Phytochemical Bulletin, 19, 11. ; Martin (2004), Molecular detection of Phytophthora ramorum , the causal agent of sudden oak death in California , and two additional species commonly recovered from diseased plant material, Phytopathology, 94, 621, doi.org/10.1094/PHYTO.2004.94.6.621 ; Caprar (2014), Optimization of DNA isolation from four species of Rhododendron from Europe of Horticulture Forestry and Biotechnology, Journal, 18, 117. ; Erwin (1996), Phytophthora Diseases Worldwide APS Press St Minnesota, USA, 562. ; Csaikl (1998), Comparative analysis of different DNA extraction protocols fast universal maxi - preparation of high quality plant DNA for genetic evaluation and phylogenetic studies, Plant Molecular Biology Reporter, 16, 69, doi.org/10.1023/A:1007428009556 ; Themann (2002), Observation of Phytophthora in water recirculation systems in commercial hardy ornamental nursery stock, European Journal of Plant Pathology, 108, 337, doi.org/10.1023/A:1015614625414 ; Drábková (2002), Comparison of seven DNA extraction and amplification protocols in historical herbarium specimens of Juncaceae, Plant Molecular Biology Reporter, 20, 161, doi.org/10.1007/BF02799431 ; Terry (2002), Detection of genetically modified crops and their derivatives : critical steps in sample preparation and extraction, Journal of AOAC International, 85, 768.