TitleStem girdling affects the carbon/nitrogen imbalance and oxidative stress, and induces leafsenescence in phenological forms of beech (Fagus sylvatica)
Journal titleActa Biologica Cracoviensia s. Botanica
Keywordscarbohydrates ; C/N ratio ; Fagus sylvatica ; leaf senescence ; phenology
Divisions of PASNauki Biologiczne i Rolnicze
Girdling was applied to 5-year-old potted beech individuals of early, intermediate and late phenological forms to block assimilate export from leaves. Phloem severance caused accumulation of soluble carbohydrates and starch in leaves and increased the C/N ratio. While the hexose content increased continuously until the end of the experiment, the sucrose and starch contents peaked earlier, depending on the plant's phenological features. Different rates of chlorophyll degradation and H2O2 and TBARS (thiobarbituric acid-reactive substances) production in different phenological forms implied that phloem girdling was the source of oxidative stress and, depending on the phenological form, accelerated leaf senescence to different degrees. The variable rate of the increase in soluble carbohydrate and starch content, characteristic of the different phenological forms, had different modifying effects on the antioxidant activity in leaves. Compared with the early phenological form, the late form was characterized by a smaller increase in H2O2 and TBARS content and delayed and slowed chlorophyll and carotenoid degradation. In conjunction with the larger increase in the activity of antioxidant enzymes (catalase, ascorbate peroxidase and superoxide clismutase) induced by carbohydrate accumulation and slower carotenoid degradation, these changes led to the late form having greater resistance to oxidative stress and slower senescence.
PublisherBiological Commission of the Polish Academy of Sciences – Cracow Branch
TypeArtykuły / Articles
IdentifiereISSN 1898-0295 ; ISSN 0001-5296