Details

Title

Polyamine plays a role in subculture growth of in vitro callus of indica rice

Journal title

Acta Biologica Cracoviensia s. Botanica

Yearbook

2017

Numer

No 1

Publication authors

Keywords

browning ; callus ; gene expression ; indica rice ; polyamine

Divisions of PAS

Nauki Biologiczne i Rolnicze

Abstract

In vitro embryogenic callus is a critical factor for genetic transformation of rice, especially for indica varieties. In this study, we investigated the relationship between polyamines, including putrescine (Put), spermidine (Spd) and spermine (Spm), and callus browning, and we studied the effect of exogenous Put on callus regeneration and on the content of endogenous polyamines. In addition, the expression levels of arginine decarboxylase gene (Adcl) and S-adenosylmethionine decarboxylase gene (Samdc) in embryogenic callus were studied by quantitative Real-time PCR analysis. The results showed that the contents of endogenous Put and Spd in the browning callus were significantly lower than those in normal callus. Exogenous Put could effectively improve the growing state of callus of indica rice and enhance the development of embryogenic callus. The content of endogenous polyamines in embryogenic callus, especially Spd and Spm, was increased after addition of exogenous Put. Additionally, exogenous Put also had an obvious impact on the expression levels of Adcl but partial effect on the expression levels of Samdc gene. This study could increase the knowledge of both embryogenic callus induction and polyamine catabolism in callus in indica rice.

Publisher

Biological Commission of the Polish Academy of Sciences – Cracow Branch

Date

2017.06.30

Type

Artykuły / Articles

Identifier

eISSN 1898-0295 ; ISSN 0001-5296

DOI

10.1515/abcsb-2017-0001

×