Comparison of the oxidative stress response of two Antarctic fungi to different growth temperatures

Journal title

Polish Polar Research




vol. 38


No 3

Publication authors


Antarctic ; antioxidant enzymes ; fungi ; glycogen ; Livingston Island ; physiological cell response ; trehalose

Divisions of PAS

Nauki o Ziemi




Two fungal strains, isolated from Livingston Island, Antarctica (Penicillium commune 161, psychrotolerant and Aspergillus glaucus 363, mesophilic) were investigated for a relationship between growth temperature and oxidative stress response. Cultivation at temperatures below - (10 and 15°C and 10 and 20°C for P. commune and A. glaucus, respectively) and above (25°C and 30°C for P. commune and A. glaucus, respectively) the optimum caused significant difference in growth and glucose uptake in comparison with the control cultures. Enhanced level of reserve carbohydrates (glycogen and trehalose) was determined under cultivation at different temperatures from the optimal one. While the highest content of trehalose was found in the exponential phase, glycogen accumulation was observed in the stationary phase when growth conditions deteriorate. The growth at temperature below- and above-optimum caused strain-dependent changes in two antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). While SOD activity in the psychrotolerant strain increases with decreasing of growth temperature, the mesophilic A. glaucus demonstrated marked reduction of it at below- and above-optimal temperature. Decreasing trend of CAT activity was observed in both strains below the optimal temperature indicating a lack of antioxidant protection from this enzyme under the cold stress conditions.


Polish Academy of Sciences ; Committee on Polar Research




Artykuły / Articles


ISSN 0138-0338 ; eISSN 2081-8262


WeinsteinR (2000), Influence of growth temperature on lipid and soluble carbohydrate synthesis by fungi isolated from fellfield soil in the maritime Antarctic, Mycologia, 222. ; BeersR (1952), spectrophotometric method for measuring the breakdown of hydrogene peroxide by catalase The of, Journal Biological Chemistry, 195. ; CoxF (2016), Not poles apart Antarctic soil fungal communities show similarities to those of the distant, Arctic Ecology Letters, 19, 528. ; TsujiM (2016), Cold - stress responses in the Antarctic basidiomycetous yeastMrakia blollopis Open, Royal Society Science, 3. ; OnofriS (null), Survival of Antarctic cryptoendolithic fungi in simulated martian conditions on board the international space station, Astrobiology, 15, 2015. ; WangM (null), fungi from the world s roof Molecular of Fungi, Persoonia Evolution, 2015. ; FrisvadJ (2008), Fungi in cold ecosystems In eds From to Biotechnology Verlag, Biodiversity, 137. ; SharmaP (2012), Reactive oxygen species oxidative damage and antioxidative defense mechanism in plants under stressful conditions of Article ID, Journal Botany, 1. ; SelbmanL (2014), Black yeast in cold habitats In eds Cold adapted Yeasts Adaptation Strategies and Biotechnological Significance, Biodiversity, 173. ; DreesensL (2014), The distribution and identity of edaphic fungi in the McMurdo Dry Valleys Biology, null, 466. ; BeauchampC (1971), improved assays and an assay applicable to acrylamide gels, Superoxide dismutase Analytical Biochemistry, 276. ; AbrashevR (2008), Oxidative stress associated impairment of glucose and ammonia metabolism in the filamentous fungus nigerB, Mycological Research, 1. ; Ellis EvansJ (1985), The interaction of soil and lake microflora at Signy Island In Laws eds Nutrient Cyclesand Food Webs Springer Verlag, null, 662. ; FeniceM (2012), Combined effects of agitation and aeration on the chitinolytic enzymes production by the Antarctic fungusLecanicillium muscariumCCFEE, Microbial Cell Factories, 11, 5003. ; DaSilvaC (2008), DaCostaMoratoNeryD The role of trehalose and its transporter in protection against reactive oxygen species, Biochimica et Biophysica Acta, 1408. ; DosReisT (2013), Identification of Glucose Transporters inAspergillus nidulans ONE, null, 8. ; AngelovaM (1996), Effect of cultural conditions on the synthesis of superoxide dismutase byHumicola lutea of Fermentation and, Journal Bioengineering, 110. ; RuisiS (2007), Fungi in in Bio, Reviews Environmental Science Technology, 127. ; GochevaY (2006), response of Antarctic and temperate strains ofPenicilliumspp to different growth temperature, Cell Mycological Research, 110. ; ParrouJ (1997), Effects of various types of stress on the metabolism of reserve carbohydrates inSaccharomyces cerevisiae : genetic evidence for a stress induced recycling of glycogen and trehalose, Microbiology, 143. ; KostadinovaN (2009), Isolation and identification of filamentous fungi from island Biotechnology, Biotechnological Equipment, 23, 267. ; SchadeB (2004), Cold adaptation in budding yeast of the, Molecular Biology Cell, 15, 5492. ; KanwalS (2011), Effect of reserve carbohydrates on oxidative stress in yeastSaccharomyces cerevisiaeY Current Research Journal of Biological, Sciences, 6210. ; ParkC (null), Algal and fungal diversity in Antarctic lichens The of, Journal Eukaryotic Microbiology, 2015. ; SomogyM (1952), on sugar determination The of, Notes Journal Biological Chemistry, 195. ; ZhangT (null), and distribution of lichen associated fungi in the Region High as revealed by pyrosequencing Scientific Reports, Diversity Arctic, 2015. ; NonzomS (2014), Fate of mitosporic soil fungi in cold deserts reviewAmerican of Research in Formal Natural, International Journal Applied Sciences, 9, 01. ; RaoS (2012), Low diversity fungal assemblage in an Antarctic Dry Valleys soil, Polar Biology, 567. ; ArenzB (2006), diversity in soils and historic wood from the Ross Sea region of Soil Biology, Fungal Biochemistry, 3057. ; ShilovaN (1989), The effect of aeration on the activity of alcohol oxidase and enzymes utilising hydrogen peroxide in the course ofCandida maltosagrowth on paraffin in, null, 430. ; FeniceM (2016), The psychrotolerant Antarctic fungusLecanicillium muscariumCCFEE a powerful producer of cold tolerant chitinolytic enzymes, Molecules, 21, 5003. ; OnofriS (2005), and The of continental Reports, null, 11. ; SofoA (null), peroxidase and catalase activities and their genetic regulation in plants subjected to drought and salinity stresses, International Journal of Molecular Sciences, 16, 2015. ; AngelovaM (1995), Effect of glucose on the superoxide dismutase production in fungal strainHumicola lutea of, Canadian Journal Microbiology, 978. ; NimseS (null), Free radicals natural antioxidants and their reaction mechanismsRSC Advances, null, 2015. ; FellerG (2006), Enzymes from psychrophilic organisms, FEMS Microbiology Reviews, 18, 189. ; AguileraJ (2007), Cold response inSaccharomyces cerevisiae : new functions for old mechanisms, FEMS Microbiology Reviews, 31, 327. ; ZhangL (2003), Growth temperature downshift induces antioxidant response inSaccharomyces cerevisiae and, Biochemical Biophysical Research Communications, 307. ; KostadinovaN (2011), Cold stress in Antarctic fungi targets enzymes of the glycolytic pathway and tricarboxylic acid cycle Biotechnology, Biotechnological Equipment, 25. ; GonçalvesV (2012), and distribution of fungal communities in lakes of, Diversity FEMS Microbiology Ecology, 459. ; MatsumotoM (2016), Molds The Battle Under Snow Between Fungal Pathogens and Their Plant Hosts Media, Science Business, 136. ; KostadinovaN (2012), Transient cold shock induces oxidative stress events in Antarctic fungi In ed Oxidative Stress, InTech, 1. ; RussellN (2006), microorganisms coming in from the cold Culture, null, 27, 1. ; IordachescuM (2011), and abiotic stress in biological systems In eds Stress in Plants Mechanisms and Adaptations, InTech, 215. ; MargesinR (2009), Effect of temperature on growth parameters of psychrophilic bacteria and yeasts, Extremophiles, 13, 257. ; ChuX (2016), Effect of arbuscular mycorrhizal fungi inoculation on cold stress induced oxidative damage in leaves ofElymus nutansGriseb, South African Journal of Botany, 21. ; FeofilovaE (2000), Different mechanisms of the biochemical adaptation of mycelial fungi to temperature stress : Changes in the cytosol carbohydrate composition, Microbiology, 69. ; FridovichI (1998), Oxygen toxicity a radical explanation of, Journal Experimental Biology, 201. ; SterflingerK (2006), yeast meristematic fungi diversity identification In eds The Handbook Ecophysiology of Yeasts, ecology Yeast Biodiversity, 501. ; LowryO (1951), Protein measurement with the Folin phenol reagent The of, Journal Biological Chemistry, 193. ; GochevaY (2009), Temperature downshift induces antioxidant response in fungi isolated from Antarctica, Extremophiles, 13, 273. ; OnofriS (2008), Resistance of Antarctic black fungi and cryptoendolithic communities to simulated space and Martian conditions in, Studies Mycology, 61. ; FukunagaN (1990), Membrane lipid composition and glucose uptake in two psychrotolerant bacteria from Antarctica of, Journal General Microbiology, 136. ; CantrellS (2011), Unusual fungal niches, Mycologia, 103. ; NewshamK (2009), and dark septate root endophytes in polar regions, Fungal Ecology, 10. ; WillekensH (1997), Catalase is a sink for and is indispensable for stress defense in plants Organisation, European Molecular Biology, 16, 4806. ; SabriA (2000), Effect of temperature on growth of psychrophilic and psychrotrophic members ofRhodotorula aurantiaca and, Applied Biochemistry Biotechnology, 84. ; GonçalvesV (2013), solitum mesophilic psychrotolerant fungus present in marine sediments from, Polar Biology, 1823. ; SangW (2008), Differential responses of the activities of antioxidant enzymes to thermal stresses between two invasive eupatorium species in China, Journal of Integrative Plant Biology, 393.