Details

Title

Identification and phenotypic plasticity of Pseudanabaena catenata from the Svalbard archipelago

Journal title

Polish Polar Research

Yearbook

2017

Volume

vol. 38

Numer

No 4

Publication authors

Keywords

16S rRNA ; Arctic ; Cyanobacteria ; Polyphasic approach ; Pseudanabaena

Divisions of PAS

Nauki o Ziemi

Coverage

445-458

Abstract

A filamentous benthic cyanobacteria, strain USMAC16, was isolated from the High Arctic Svalbard archipelago, Norway, and a combination of morphological, ultrastructural and molecular characterisation (16S rRNA gene sequence) used to identify to species level. Cell dimensions, thylakoid arrangement and apical cell shape are consistent with the Pseudanabaena genus description. The molecular characterisation of P. catenata gave 100% similarity with Pseudanabaena catenata SAG 1464-1, originally reported from Germany. Strain USMAC16 was cultured under a range of temperature and photoperiod conditions, in solid and liquid media, and harvested at exponential phase to examine its phenotypic plasticity. Under different culture conditions, we observed considerable variations in cell dimensions. The longest cell (5.91±0.13 μm) was observed at 15°C under 12:12 light:dark, and the widest cell (3.24±0.06 μm) at 4°C under 12:12 light: dark in liquid media. The study provides baseline data documenting the morphological variation of P. catenata in response to changing temperature regimes.

Publisher

Committee on Polar Research ; Polish Academy of Sciences

Date

2017.12.15

Type

Artykuły / Articles

Identifier

ISSN 0138-0338 ; eISSN 2081-8262

DOI

10.1515/popore-2017-0022

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