Pre-treatment of Fulvic Acid Plays a Stimulant Role in Protection of Soybean (Glycine max L.) Leaves Against Heat and Salt Stress
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<jats:title>Abstract</jats:title> <jats:p>The purpose of this study was to illuminate the effects of fulvic acid in plants’ stress signaling pathway. 2.0 mg/l fulvic acid was sprayed on soybean leaves for 3 days at 12 h intervals, followed by treatment of 150 mM NaCl or exposed to heat stress at 35°C for 2 h over 2 days. Pre-treatment with fulvic acid increased the relative water content (RWC), antioxidant enzyme, isoenzyme activities (SOD, APX, GST), as well as alleviated the stress-induced oxidative damage by decreasing the levels of hydrogen peroxide (H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>) and malondialdehyde (MDA). In addition, the application of fulvic acid under salt stress induced <jats:italic>rubisco</jats:italic> expression only at 12 h, while it induced the expression of <jats:italic>cytochrome c oxidase</jats:italic> at 6 h and 12 h. On the other hand, fulvic acid under heat stress induced significant expression of both rubisco and <jats:italic>cytochrome c oxidase</jats:italic> at 6 h and 12 h. However, under high salinity conditions, fulvic acid suppressed the transcript levels of <jats:italic>Hsp70</jats:italic>, while it induced increases in <jats:italic>Hsp70</jats:italic> levels under heat treatment at 6 h. As a result, in this study, fulvic acid played the role of a regulator and stimulant in stress response of soybean leaves.</jats:p>
eISSN 1898-0295 ; ISSN 0001-5296
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