Methyl Jasmonate Elicitation Affects Expression of Genes Involved in Biosynthesis and Turnover of 2-Phenylethylamine in Maize Seedlings

Journal title

Acta Biologica Cracoviensia s. Botanica




No 1

Publication authors

Divisions of PAS

Nauki Biologiczne i Rolnicze


<jats:title>Abstract</jats:title> <jats:p>The objective of the study was to assess the influence of methyl jasmonate (MJ) vapors on accumulation of 2-phenylethylamine (PEA), phenylacetic acid (PAA) and 2-phenylethanol (PE) in leaves and roots of maize (<jats:italic>Zea mays</jats:italic> L. subsp. <jats:italic>mays</jats:italic>, <jats:italic>saccharata</jats:italic> group, cv. Złota Karłowa) seedlings. Furthermore, we analyzed the expression patterns of eight genes (<jats:italic>ADH1</jats:italic>, <jats:italic>ADH2</jats:italic>, <jats:italic>AO2</jats:italic>, <jats:italic>CAO</jats:italic>, <jats:italic>PDC1</jats:italic>, <jats:italic>PDC2</jats:italic>, <jats:italic>PTA</jats:italic> and <jats:italic>LOX</jats:italic>, encoding alcohol dehydrogenase 1 and 2, primary amine oxidase, aldehyde oxidase 2, phenylalanine decarboxylase 1 and 2, phenylalanine (histidine) transaminase and lipoxygenase, respectively) involved in biosynthesis and turnover of PEA in maize tissues. In addition, the effect of MJ application on fresh biomass and growth of the tested seedlings was recorded. One-day MJ exposure increased the fresh weight of aerial parts and roots of <jats:italic>Z. mays</jats:italic> seedlings, whereas the opposite tendency occurred after 4-day of MJ treatment. One-day application of MJ resulted in an increase in the length of roots and its fluctuations in the aerial parts of maize plants, but extended exposure declined the growth of both parts of the seedlings. Methyl jasmonate elicitation caused various changes in the contents of PEA, PAA and PE in the maize seedlings. MJ treatments led to high upregulation of most genes, with the exception of three genes (i.e., <jats:italic>ADH1</jats:italic>, <jats:italic>ADH2</jats:italic> and <jats:italic>AO2</jats:italic>) whose expression was downregulated after a 4-day exposure.</jats:p>


Biological Commission of the Polish Academy of Sciences – Cracow Branch




eISSN 1898-0295 ; ISSN 0001-5296


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