Details

Title

Methyl Jasmonate Elicitation Affects Expression of Genes Involved in Biosynthesis and Turnover of 2-Phenylethylamine in Maize Seedlings

Journal title

Acta Biologica Cracoviensia s. Botanica

Yearbook

2016

Numer

No 1

Publication authors

Divisions of PAS

Nauki Biologiczne i Rolnicze

Abstract

Abstract The objective of the study was to assess the influence of methyl jasmonate (MJ) vapors on accumulation of 2-phenylethylamine (PEA), phenylacetic acid (PAA) and 2-phenylethanol (PE) in leaves and roots of maize (Zea mays L. subsp. mays, saccharata group, cv. Złota Karłowa) seedlings. Furthermore, we analyzed the expression patterns of eight genes (ADH1, ADH2, AO2, CAO, PDC1, PDC2, PTA and LOX, encoding alcohol dehydrogenase 1 and 2, primary amine oxidase, aldehyde oxidase 2, phenylalanine decarboxylase 1 and 2, phenylalanine (histidine) transaminase and lipoxygenase, respectively) involved in biosynthesis and turnover of PEA in maize tissues. In addition, the effect of MJ application on fresh biomass and growth of the tested seedlings was recorded. One-day MJ exposure increased the fresh weight of aerial parts and roots of Z. mays seedlings, whereas the opposite tendency occurred after 4-day of MJ treatment. One-day application of MJ resulted in an increase in the length of roots and its fluctuations in the aerial parts of maize plants, but extended exposure declined the growth of both parts of the seedlings. Methyl jasmonate elicitation caused various changes in the contents of PEA, PAA and PE in the maize seedlings. MJ treatments led to high upregulation of most genes, with the exception of three genes (i.e., ADH1, ADH2 and AO2) whose expression was downregulated after a 4-day exposure.

Publisher

Biological Commission of the Polish Academy of Sciences – Cracow Branch

Date

2016

Identifier

eISSN 1898-0295 ; ISSN 0001-5296

References

HorbowiczM (2011), The effect of methyl jasmonate on accumulation of - phenylethylamine and putrescine in seedlings of common buckwheat ( Fagopyrum esculentum ), Acta Physiologiae Plantarum, 33, 897, doi.org/10.1007/s11738-010-0616-5 ; KuśPM (2013), The volatile profiles of a rare apple ( Malus domesticaBorkh ) honey : shikimic acid - pathway derivatives terpenes and others Chemistry and, Biodiversity, 10, 1638, doi.org/10.1002/cbdv.201200404 ; Ruiz (2011), Methyl jasmonate induces ATP biosynthesis deficiency and accumulation of proteins related to secondary metabolism inCatharanthus roseus hairy roots, Plant and Cell Physiology, 52, 1401, doi.org/10.1093/pcp/pcr086 ; ConchaCM (2013), Methyl jasmonate treatment induces changes in fruit ripening by modifying the expression of several ripening genes inFragaria chiloensisfruit, Plant Physiology and Biochemistry, 70, 433, doi.org/10.1016/j.plaphy.2013.06.008 ; WightmanF (1982), Identification of phenylacetic acid as a natural auxin in the shoots of higher plants, Physiologia Plantarum, 55, 17, doi.org/10.1111/j.1399-3054.1982.tb00278.x ; WatanabeS (2002), Biogenesis of - phenylethanol in rose flowers : incorporation of phenylalanine into - phenylethanol and itsβ - D - glucopyranoside during the flower opening ofRosa Hoh - Jun andRosa damascenaMill Bioscience , Biotechnology and, Biochemistry, 2, 943, doi.org/10.1271/bbb.66.943 ; LivakKJ (2001), Analysis of relative gene expression data using real - time quantitative PCR and the ΔΔCtmethod, Methods, 25, 402, doi.org/10.1006/meth.2001.1262 ; MaedaH (2010), RNAi suppression ofArogenate Dehydratase reveals that phenylalanine is synthesized predominantly via the arogenate pathway in petunia petals, Plant Cell, 22, 832, doi.org/10.1105/tpc.109.073247 ; OwenCA (2004), Expression of genes for alcohol dehydrogenase and pyruvate decarboxylase in petals of cut carnation flowers in response to hypoxia and anoxia, Physiologia Plantarum, 122, 412, doi.org/10.1111/j.1399-3054.2004.00423.x ; ChenXM (2011), Functional characterization of rose phenylacetaldehyde reductase ( PAR ) , an enzyme involved in the biosynthesis of the scent compound - phenylethanol, Journal of Plant Physiology, 168, 88. ; ZhangS (2014), Selective responses of enzymes in the two parallel pathways of rosmarinic acid biosynthetic pathway to elicitors inSalvia miltiorrhizahairy root cultures, Journal of Bioscience and Bioengineering, 117, 645, doi.org/10.1016/j.jbiosc.2013.10.013 ; YesbergenovaZ (2005), The plant Mo - hydroxylases aldehyde oxidase and xanthine dehydrogenase have distinct reactive oxygen species signatures and are induced by drought and abscisic acid, Plant Journal, 42, 862, doi.org/10.1111/j.1365-313X.2005.02422.x ; PanQ (2012), Molecular cloning and expression of gene encoding aromatic amino acid decarboxylase in Vidal blanc grape berries, Molecular Biology Reports, 39, 4319, doi.org/10.1007/s11033-011-1219-y ; SakaiM (2007), Production of - phenylethanol in roses as the dominant floral scent compound froml - phenylalanine by two key enzymes , a PLP - dependent decarboxylase and a phenylacetaldehyde reductase Bioscience , Biotechnology and, Biochemistry, 71, 2408, doi.org/10.1271/bbb.70090 ; Planas (2013), Copper - containing amine oxidases contribute to terminal polyamine oxidation in peroxisomes and apoplast ofArabidopsis thaliana, BMC Plant Biology, 13, 109, doi.org/10.1186/1471-2229-13-109 ; PauwelsL (2008), Mapping methyl jasmonate - mediated transcriptional reprogramming of metabolism and cell cycle progression in culturedArabidopsiscells Proceedings of the National Academy of, Sciences USA, 105, 1380, doi.org/10.1073/pnas.0711203105 ; HirataH (2012), Functional characterization of aromatic amino acid aminotransferase involved in - phenylethanol biosynthesis in isolated rose petal protoplasts, Journal of Plant Physiology, 169, 444, doi.org/10.1016/j.jplph.2011.12.005 ; HorbowiczM (2015), Methyl jasmonate stimulates biosynthesis of - phenylethylamine , phenylacetic acid and - phenylethanol in seedlings of common buckwheat, Acta Biochimica Polonica, 62, 235, doi.org/10.18388/abp.2014_929 ; Zdunek (2013), Expression ofPisum sativumPsAO gene , which encodes an aldehyde oxidase utilizing abscisic aldehyde , is induced under progressively but not rapidly imposed drought stress, Plant Physiology and Biochemistry, 71, 3, doi.org/10.1016/j.plaphy.2013.06.027 ; TomèF (1975), Distribution of phenylalanine transaminase and phenylalanine ammonialyase activities in etiolated and light irradiated radish seedlings ( Raphanus sativusL ), Experientia, 31, 1119, doi.org/10.1007/BF02326743 ; FacchiniPJ (2000), Plant aromaticl - amino acid decarboxylases : evolution biochemistry regulation and metabolic engineering applications, Phytochemistry, 54, 121, doi.org/10.1016/S0031-9422(00)00050-9 ; SimonS (2011), Why plants need more than one type of auxin, Plant Science, 180, 454, doi.org/10.1016/j.plantsci.2010.12.007 ; KimE (2003), Dual positional specificity and expression of non - traditional lipoxygenase induced by wounding and methyl jasmonate in maize seedlings, Plant Molecular Biology, 52, 1203, doi.org/10.1023/B:PLAN.0000004331.94803.b0 ; LeThiH (2014), Isolation and identification of an allelopathic phenylethylamine in rice, Phytochemistry, 108, 109, doi.org/10.1016/j.phytochem.2014.08.019 ; ChenJ (2013), Biosynthesis of the active compounds ofIsatis indigoticabased on transcriptome sequencing and metabolites profiling, BMC Genomics, 14, 857, doi.org/10.1186/1471-2164-14-857 ; ThorpeMR (2007), imaging : methyl jasmonate moves in both phloem and xylem , promotes transport of jasmonate , and of photoassimilate even after proton transport is decoupled, Planta, 11, 541, doi.org/10.1007/s00425-007-0503-5 ; ZareiA (2015), Apple fruit copper amine oxidase isoforms : peroxisomal MDAO prefers diamines as substrates , whereas extracellular MDAO exclusively utilizes monoamines, Plant and Cell Physiology, 56, 137. ; GharechahiJ (2013), An integrated proteomic approach to decipher the effect of methyl jasmonate elicitation on the proteome ofSilybum marianumL hairy roots, Plant Physiology and Biochemistry, 70, 115, doi.org/10.1016/j.plaphy.2013.05.031 ; JerkovićI (2010), Volatile composition screening ofSalixspp nectar honey : benzenecarboxylic acids norisoprenoids terpenes and others Chemistry and, Biodiversity, 7, 2309, doi.org/10.1002/cbdv.201000021 ; KaminagaY (2006), Plant phenylacetaldehyde synthase is a bifunctional homotetrameric enzyme that catalyzes phenylalanine decarboxylation and oxidation, Journal of Biological Chemistry, 281, doi.org/10.1074/jbc.M602708200 ; TiemanD (2006), Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles - phenylethanol and - phenylacetaldehyde Proceedings of the National Academy of, Sciences USA, 2, 8287, doi.org/10.1073/pnas.0602469103 ; TiemanDM (2007), Tomato phenylacetaldehyde reductases catalyze the last step in the synthesis of the aroma volatile - phenylethanol, Phytochemistry, 2, 2660, doi.org/10.1016/j.phytochem.2007.06.005 ; PietrangeliP (2007), Substrate specificity of copper - containing plant amine oxidases, Journal of Inorganic Biochemistry, 101, 997, doi.org/10.1016/j.jinorgbio.2007.03.014 ; PetersJS (2004), Relationship between alcohol dehydrogenase activity and low - temperature in two maize genotypes andAdh Adh doubly null, Plant Physiology and Biochemistry, 42, 841, doi.org/10.1016/j.plaphy.2004.10.004 ; KlemaVJ (2012), The role of protein crystallography in defining the mechanisms of biogenesis and catalysis in copper amine oxidase, International Journal of Molecular Sciences, 13, 5375, doi.org/10.3390/ijms13055375 ; PathuriIP (2011), Alcohol dehydrogenase of barley modulates susceptibility to the parasitic fungusBlumeria graminisf sp hordei, Journal of Experimental Botany, 62, 3449, doi.org/10.1093/jxb/err017 ; SmithTA (1977), Phenethylamine and related compounds in plants, Phytochemistry, 16, 9, doi.org/10.1016/0031-9422(77)83004-5 ; ZhangL (2008), Methyl jasmonate induces production of reactive oxygen species and alterations in mitochondrial dynamics that precede photosynthetic dysfunction and subsequent cell death, Plant and Cell Physiology, 49, 1092, doi.org/10.1093/pcp/pcn086 ; GondaI (2010), Branched chain and aromatic amino acid catabolism into aroma volatiles inCucumis meloL fruit, Journal of Experimental Botany, 61, 1111, doi.org/10.1093/jxb/erp390

DOI

10.1515/abcsb-2016-0005

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