Details

Title

Nuclear DNA Content and Ploidy Level of Apple Cultivars Including Polish ones in Relation to Some Morphological Traits

Journal title

Acta Biologica Cracoviensia s. Botanica

Yearbook

2016

Numer

No 1

Publication authors

Divisions of PAS

Nauki Biologiczne i Rolnicze

Abstract

<jats:title>Abstract</jats:title> <jats:p>Apple species and cultivars differ in nuclear (2C) DNA content and ploidy level. The majority of these genotypes are diploids, but there are some triploids and a few tetraploids. Nuclear DNA content is a specific feature and its flow cytometric evaluation can be helpful in differentiating taxa. For many apple genotypes – including all the Polish ones, these characteristics are not known. 2C DNA was evaluated in relation to leaf, flower, fruit, pollen grain and stomata sizes as well as to the flowering time for seventy genotypes (including 46 Polish cultivars) gathered in the gene bank of the Research Institute of Horticulture, Skierniewice, Poland. For standard cultivars with the known chromosome number, 2C value was 1.71 pg for diploid cultivar ‘Alwa’ (2n=2x=34), 2.55 pg for triploid ‘Boskoop’ (3x=51), and 3.37 pg for tetraploid genome (4x=68) of mixoploid ‘McIntosh 2x+4x’. In 61 cultivars (including 41 Polish ones), the nuclear DNA content ranged from 1.58 to 1.78 pg indicating their diploid chromosome number. Five cultivars were identified as triploids (‘Bursztówka Polska’, ‘Pagacz’, ‘Rapa Zielona’, ‘Rarytas Śląski’ and ‘Witos’) owing to their nuclear DNA amount ranging between 2.42 and 2.58 pg. Leaf, flower, fruit, stomata and pollen grain sizes were on average significantly larger in triploids. Thus, in 3x plants the mean leaf surface was 49.1 cm<jats:sup>2</jats:sup>, flower diameter – 52.4 mm, fruit weight – 204.7 g, stomata length – 32.1 μm and pollen grain diameter – 33.7 μm, whereas in diploids – 36.0 cm<jats:sup>2</jats:sup>, 46.1 mm, 162.7 g, 28.4 μm and 30.7 μm, respectively. Pollen grain viability was on average significantly higher in diploids (75.6%), compared to triploids (22%). These results confirm that in apple, as in many other plant species, the higher ploidy level of triploids is generally associated with increased sizes of pollen grains, stomata, flowers, fruits and leaves but decreased pollen viability. No clear correlation between ploidy level and flowering time was found. In the case of mixoploid apple genotypes possessing diploid and tetraploid genomes, some phenotype observation is helpful in describing the ploidy level of the histogenic layers, L1 and L2. Small stomata sizes (similar to diploid) indicate diploid L1 and larger leaf sizes, compared to diploid counterparts, show tetraploid L2. The results will be used for breeding, in which it is important to determine maternal and paternal genotypes as well as the direction of the crossing that is of great importance in obtaining seeds and materials for further selection.</jats:p>

Publisher

Biological Commission of the Polish Academy of Sciences – Cracow Branch

Date

2016

Identifier

eISSN 1898-0295 ; ISSN 0001-5296

References

NilesWL (1992), Pollen germination of rhizoma peanut cv Florigraze, Peanut Science, 19. ; PodwyszyńskaM (2015), Phenotypic and genome size changes ( variation ) in synthetic tetraploids of daylily ( Hemerocallis ) in relation to their diploid counterparts, Euphytica, 203. ; WachiraFN (1994), Triploidy in tea sinensis ) : effects of yield and yield attributes of, Journal Horticultural Science, 69. ; TatumTC (2005), Variation in nuclear DNA kontent inMalusspecies and cultivated apples, Genome, 48. ; ArisumiT (1964), Colchicine - induced tetraploid and cytochimeral daylilies of, Journal Heredity, 55. ; Pereira (2007), Evaluation of genetic identity and variation of local apple cultivars ( Malus domesticaBorkh ) from Spain using microsatellite markers Resources and, Genetic Crop Evolution, 54. ; ZonneveldBJM (2009), The systematic value of nuclear genome size for all species ofTulipaL ( Liliaceae and, Plant Systematics Evolution, 281. ; González (2013), Physiological behaviour of mangos with different ploidy levels, Acta Horticulturae, 992. ; SedyshevaGA (2013), Estimation of new tetraploid apple forms as donors of diploid gametes for selection on a polyploidy level Universal Journal of, Plant Science, 1. ; RamannaMS (2003), Relevance of sexual polyploidization for crop improvement a review, Euphytica, 133. ; WebsterAD (1996), Cherry rootstock evaluation at East Malling, Acta Horticulturae, 410. ; BlankeMM (1994), Stomata and structure of tetraploid apple leaves culturedin vitro of, Annals Botany, 73. ; KorbanSS (2009), Genome size and nucleotypic variation inMalusgermplasm, Genome, 52. ; RogalskaSM (2007), Mechanizmy zmian genomowych i zmian w ekspresji genów w mieszańcowych poliploidach roślin, Kosmos, 56. ; VandenhoutH (1995), Effect of ploidy on stomatal and other quantitative traits in plantain and banana hybrids, Euphytica, 83. ; CraneMB (1930), Fertility and vigour of apples in relation to chromosome number of, Journal Genetics, 22. ; Ramos (2007), Morphology and microsatellites in Spanish apple collections of and Biotechnology, Journal Horticultural Science, 82, 257. ; JaskaniMJ (2005), Comparative study on vegetative reproductive and qualitative traits of seven diploid and tetraploid watermelon lines, Euphytica, 145. ; AnssourS (2009), Phenotypic genetic and genomic consequences of natural and synthetic polyploidization of Nicotiana attenuata and Nicotiana obtusifolia of, Annals Botany, 103. ; HöferM (2010), Genome size variation inMalusspecies of, Journal Botany, 1. ; DicksonEE (1992), Nuclear DNA content variation within the Rosaceae of, American Journal Botany, 1081, doi.org/10.2307/2444917 ; JędrzejczykI (2010), Leaves and seeds as materials for flow cytometric estimation of the genome size of Rosaceae woody species containing DNA - staining inhibitors of, Journal Botany, 11, 1, doi.org/10.1155/2010/930895 ; LysákMA (1998), Estimation of nuclear DNA content inSesleria ( Poaceae ), Caryologia, 51. ; DzialukA (2007), Presence of triploids among oak species of, Annals Botany, 99. ; PodwyszyńskaM (2011), Histogenic identification by cytological analysis of colchicine - induced polyploids ofHemerocallis, Acta Horticulturae, 886. ; ZonneveldBJM (2007), Nuclear DNA content of ploidy chimeras ofHostaTratt ( Hostaceae ) demonstrate three apical layers in all organs , but not in the adventitious root and, Plant Systematics Evolution, 269. ; RybinVA (1926), Cytological investigations of the genusMalus ( preliminary account ) of and Plant Breeding ( Leningrad, Bulletin Applied Botany, 16, 187. ; ArumuganathanK (1991), Nuclear DNA content of some important plant species Molecular, Plant Biology Reporter, 9. ; BisogninC (2009), Use of SSR markers to assess sexual vs apomictic origin and ploidy level of breeding progeny derived from crosses of apple proliferation - resistantMalus sieboldiiand its hybrids withMalus domesticacultivars, Plant Breeding, 507, doi.org/10.1111/j.1439-0523.2008.01614.x ; LespinasseY (1986), Contribution to the study of a haploid apple ( Malus pumila ) Descriptive study and comparison with clones of different levels of ploidy Vegetative characters : internodes , leaves and stomata, Agronomie, 1. ; GalbraithDW (1983), Rapid flow cytometric analysis of the cell cycle in intact plant tissues, Science, 220. ; ElradiT (2010), Production of colchicine - induced tetraploids inVicia villosaRoth, Caryologia, 63.

DOI

10.1515/abcsb-2016-0008

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