Establishment of Highly Efficient Agrobacterium Rhizogenes-mediated Transformation for Stevia Rebaudiana Bertoni Explants
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<jats:title>Abstract</jats:title> <jats:p>Leaves and internodes from <jats:italic>Stevia rebaudiana</jats:italic> Bertoni plants growing in different conditions were used for transformation with two strains of <jats:italic>Agrobacterium rhizogenes</jats:italic>: ATCC 15384 and LBA 9402. Hairy roots formation was observed and the percentage of the transformed explants depended on the type of explant, time of inoculation and inoculum concentration. Inoculation of explants from <jats:italic>ex vitro</jats:italic> and <jats:italic>in vitro</jats:italic> plants with LBA 9402 strain led to higher efficiency of transformation than inoculation with ATCC 15384 strain. Growth rate of hairy roots in liquid culture was assessed under light and dark conditions. It was found that the growth of hairy roots decreased significantly under light conditions. Transformation of hairy roots growing in different culture conditions was confirmed at the molecular level using PCR method with primers constructed against <jats:italic>rolB</jats:italic> and <jats:italic>rolC</jats:italic> genes from <jats:italic>A. rhizogenes</jats:italic>.</jats:p>
eISSN 1898-0295 ; ISSN 0001-5296
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