Tytuł artykułu

Thallium Hyperaccumulation in Polish Populations of Biscutella laevigata (Brassicaceae)

Tytuł czasopisma

Acta Biologica Cracoviensia s. Botanica




No 2

Autorzy publikacji

Wydział PAN

Nauki Biologiczne i Rolnicze


<jats:title>Abstract</jats:title> <jats:p><jats:italic>Biscutella laevigata</jats:italic> L. is known as a Tl hyperaccumulator. In Poland <jats:italic>Biscutella laevigata</jats:italic> occurs in the Tatra Mts (Western Carpathians) and on the calamine waste heap in Bolesław near Olkusz (Silesian Upland). The purpose of this work was to evaluate whether plants of both populations were able to accumulate an elevated amount of thallium in their tissues. The plants were cultivated in calamine soil in a glasshouse for a season and studied at different ages – from 2-week-old seedlings to 10-month-old adults. Additionally, the plants were grown for ten weeks in calamine soil with EDTA to enhance Tl bioavailability. The total content of Tl in plant tissues after digestion was determined by ICP-MS, whereas its distribution in leaves was studied by LA-ICP-MS. Of the total content of Tl in the soil in the range of (15.2–66.7) mg∙kg<jats:sup>−1</jats:sup>d.m., only (1.1–2.1) mg∙kg<jats:sup>−1</jats:sup>d.m. was present in a bioavailable form. The mean content in all the plants grown on the soil without EDTA was 98.5 mg∙kg<jats:sup>−1</jats:sup>d.m. The largest content was found in leaves – 164.9 mg∙kg<jats:sup>−1</jats:sup>d.m. (max. 588.2 mg∙kg<jats:sup>−1</jats:sup>d.m.). In the case of plants grown on the soil enriched with EDTA, the mean content in plants increased to 108.9 mg∙kg<jats:sup>−1</jats:sup>d.m., max. in leaves – 138.4 mg∙kg<jats:sup>−1</jats:sup>d.m. (max. 1100 mg∙kg<jats:sup>−1</jats:sup>d.m.). The translocation factor was 6.1 in the soil and 2.2 in the soil with EDTA; the bioconcentration factor amounted to 10.9 and 5.8, respectively. The plants from both populations did not contain a Tl amount clearly indicating hyperaccumulation (100–500 mg∙kg<jats:sup>−1</jats:sup>d.m.), however, high (&gt;1) translocation and bioconcentration factors suggest such an ability. It is a characteristic species-wide trait; <jats:italic>B. laevigata</jats:italic> L. is a facultative Tl hyperaccumulator. The largest Tl amount was located at the leaf base, the smallest at its top. Thallium also occurred in trichomes, which was presented for the first time; in this way plants detoxify Tl in the above-ground parts. Leaves were much more hairy in the Bolesław plants. This is an adaptation for growth in the extreme conditions of the zinc-lead waste heap with elevated Tl quantity.</jats:p>


Biological Commission of the Polish Academy of Sciences – Cracow Branch




eISSN 1898-0295 ; ISSN 0001-5296


VanDerEntA (2013), Hyperaccumulators of metal and metalloid trace elements : Facts and fiction, Plant and Soil, 362. ; DmowskiK (1998), Small mammal population and community under conditions of extremely high thallium contamination in the environment, Ecotoxicology and Environmental Safety, 41, 2, ; HeilDM (1999), Remediation of lead contaminated soil by EDTA Batch and column studies Water Air and, Soil Pollution, 113, 77, ; SagerM (1994), Thallium, Toxicological and Environmental Chemistry, 45, 11, ; WierzbickaM (2004), Highly toxic thallium in plants from the vicinity of Olkusz, Ecotoxicology and Environmental Safety, 59, 84, ; OlkoA (2008), Armeria maritimafrom a calamine heap Initial studies on physiologic - metabolic adaptations to metal - enriched soil, Ecotoxicology and Environmental Safety, 69. ; LacosteC (1999), The phytoremediation potential of thallium - contaminated soils usingIberisandBiscutellaspecies, International Journal of Phytoremediation, 1. ; ScheckelKG (2007), Synchroton X - ray absorption - edge computed microtomography imaging of thallium compartmentalization inIberis intermedia, Plant and Soil, 290. ; ScheckelKG (2004), In vivo synchrotron study of thallium speciation and compartmentation inIberis intermedia and Technology, Environmental Science, 38, 5095, ; SheoranV (2009), Phytomining review, Minerals Engineering, 22. ; KüpperH (2000), Cellular compartmentation of cadmium and zinc in relation to other metals in the hyperaccumulatorArabidopsis halleri, Planta, 212. ; BroadhurstCL (2004), a Nickel localization and response to increasing Ni soil levels in leaves of the Ni hyperaccumulatorAlyssum murale, Plant and Soil, 265. ; PošćićF (2015), Variation in heavy metal accumulation and genetic diversity at a regional scale among metallicolous and non - metallicolous populations of the facultative metallophyteBiscutella laevigatasubsp laevigata, International Journal of Phytoremediation, 17, 464, ; LacosteC (2001), Uptake of thallium by vegetables : its significance for human health phytoremediation and phytomining, Journal of Plant Nutrition, 24, 1205, ; EpsteinAL (1999), EDTA and Pb - EDTA accumulation inBrassica junceagrown in Pb - amended soil, Plant and Soil, 208. ; VanĕkA (2010), Thallium uptake by white mustard ( Sinapis albaL ) grown on moderately contaminated soils Agro - environmental implications, Journal of Hazardous Materials, 182. ; TurgutC (2004), The effect of EDTA and citric acid on phytoremediation of Cd Cr and Ni from soil usingHelianthus annuus, Environmental Pollution, 131. ; CrößmannG (1984), Thallium a new environmental problem ? Botanik, Angewdte, 58, 3. ; LiphadziMS (2003), EDTA - assisted heavy - metal uptake by poplar and sunflower grown at a long - term sewage - sludge farm, Plant and Soil, 257. ; DobrzańskaJ (1955), Badania florystyczno - ekologiczne nad roślinnością galmanową okolic Bolesławia i Olkusza Flora and ecological studies on calamine flora in the district of Bolesław and Olkusz ], Acta Societatis Botanicorum Poloniae, 24, 357. ; SereginIV (1997), Histochemical investigation of cadmium and lead distribution in plants, Russian Journal of Plant Physiology, 44. ; HanćA (2009), An analysis of long - distance root to leaf transport of lead inPisum sativumplants by laser ablation - ICP - MS, International Journal of Environmental Analytical Chemistry, 89, 651, ; PošćićF (2013), Hyperaccumulation of thallium is population - specific and uncorrelated with caesium accumulation in the thallium hyperaccumulator Biscutella laevigata, Plant and Soil, 365. ; DmowskiK (2002), Thallium contamination of selected plants and fungi in the vicinity of the Bolesław zinc smelter in Bukowno Preliminary study, Acta Biologica Cracoviensia Series Botanica, 44. ; AndersonCWN (1999), Phytominig for nickel thallium and gold, Journal of Geochemical Exploration, 67. ; PollardAJ (2014), Facultative hyperaccumulation of heavy metals and metalloids, Plant Science, 217. ; WierzbickaM (2004), Adaptation ofBiscutella laevigataL , a metal hyperaccumulator to growth o n a zinc - lead waste heap in southern Poland Differences between waste - heap and mountain populations, Chemosphere, 54. ; LeblancM (1999), The phytomining and environmental significance of hyperaccumulation of thallium byIberis intermediafrom Southern France, Economic Geology, 94. ; WenzelWW (1999), Accumulation of heavy metals in plants grown on mineralized soils of the Austrian Alps, Environmental Pollution, 104. ; XiaoT (2004), Environmental concerns related to high thallium levels in soils and thallium uptake by plants in southwest Guizhou China of the, Science Total Environment, 318. ; EscarréJ (2011), Heavy metal concentration survey in soils and plants of the Les Malines Mining District Southern implications for soil restoration Water Air and, Soil Pollution, 216. ; Szarek (2002), Concentration of alkaline and heavy metals inBiscutella laevigataL andPlantago lanceolataL growing on calamine spoils, Acta Biologica Cracoviensia Series Botanica, 44, 29. ; GodzikB (1991), Accumulation of heavy metals inBiscutella laevigata ( Cruciferae ) as a function of their concentration in the substrate, Polish Botanical Studies, 2, 241. ; BroadhurstCL (2004), Simultaneous hyperaccumulation of nickel manganese and calcium inAlyssumleaf trichomes and Technology, Environmental Science, 38. ; LisJ (2003), Thallium in soils and stream sediments of a Zn - Pb mining and smelting area and Technology, Environmental Science, 37, 4569,