Cloning, Expression and Genetic Transformation of Sucrose Phosphate Synthase (Sps) Gene in Saccharum Spontaneum L.
Divisions of PAS
<jats:title>Abstract</jats:title> <jats:p> Sucrose phosphate synthase (SPS) is a key enzyme catalyzing sucrose metabolism in plants. In this study, we isolated the SPS cDNA from Saccharum spontaneum and designated as SsSPS (GenBank accession no. MF398541). The full-length of SsSPS cDNA was 4153-bp with an opening reading frame (ORF) of 3132 nucleotides, which encoded a 1043-amino acid protein. The nucleotide sequences alignment showed that it had 98%, 97% and 87% homology with S. officinarum, Setaria italica and Lolium perenne, respectively. Moreover, the SsSPS was detected to express in leaf and stem tissues of S. spontaneum and exhibited a predominant expression in the stem tissue. However, there was no significant difference in the expression level of SsSPS between young leaves and mature ones. Additionally, we generated transgenic S. spontaneum using Agrobacterium-mediated transformation. Our data will provide a valuable foundation for further study of the potential role of SPS in plants.</jats:p>
ISSN 0001-5296 ; eISSN 1898-0295
MACRAE (2004), Evolution and function of the sucrose - phosphate synthase gene families in wheat and other grasses, Plant Physiology, 135. ; XIE (2007), An overview and perspectives of energy crop re - sources Resources, Science, 29. ; MOHAMAD (2013), Comparison of gene nature used in real - time PCR for porcine identification and quantification : a review, Food Research International, 50. ; KOMATSU (1999), Differential expression of three sucrose - phos - phate synthase isoforms during sucrose accumulation in citrus fruits ( Citrus unshiu Marc ), Plant Science, 140. ; XU (2011), Genetic diversity among sugarcane genotypes based on polymorphisms in su - crose metabolism genes Scientia Agricultura, Sinica, 44. ; YU (2007), Cloning and expression analysis of a sucrose phosphate synthase cDNA fragment in melon fruit, Acta Horticulturae Sinica, 34, 205. ; HAWKER (1971), Enzymes concerned with sucrose synthesis and transformations in seeds of maize , broad bean and castor bean, Phytochemistry, 10, 2313. ; LI (2007), Analysis of advantage and out - look on sugarcane as energy - crop Chinese Agricultural, Science Bulletin, 23. ; KLEIN (1993), CRAFTS Clon - ing and developmental expression of the sucrose - phos - phate - synthase gene from spinach, Planta, 190. ; LIU (2003), Sugar accumulation and changes of sucrose - metabolizing enzyme activities in citrus fruit, Acta Horticulturae Sinica, 30. ; HESSE (1995), Cloning and expression analysis of sucrose - phosphate synthase from sugar beet ( Beta vulgaris Molecular Genetics and, Genomics, 247. ; LIU (2005), Research advance on sucrose phosphate synthase in sucrose me - tabolism, Molecular Plant Breeding, 3.