Production and Characterization of Tissue Cultures of Four Crocus Species from the Carpathian Basin
Divisions of PAS
<jats:title>Abstract</jats:title> <jats:p> We aimed to produce tissue cultures and plant regeneration from endangered Crocus species: C. scepusiensis, C. tommasinianus, C. vittatus (“Verni” series of the genus) and C. banaticus. For initiation of cultures we used a plant growth regulator (PGR) combination used for in vitro culture of saffron and its relatives: 10 mg L-1 α-naphthaleneacetic acid (NAA) and 1 mg L-1 6-benzyladenine (BA). Shoot tips of young seedlings (C. scepusiensis) and corms (for the rest of species) were used as explants. C. scepusiensis explants developed into organogenic calli. On media with decreased NAA and with or without increased BA concentration, calli produced stigma-like structures and/or shoots and whole plants. In the other species, callus initiation medium induced callus formation with abundant somatic embryos. In C. tommasinianus, embryos developed shoots when auxin content of medium was decreased. In C. banaticus, a decrease of auxin with or without an increase in cytokinin content led to shoot or whole plant regeneration, as in C. scepusiensis. In the case of C. vittatus and C. banaticus, initiation and/or maintenance of cultures on indole-3-butyric acid (IBA) and increased sucrose concentration stimulated whole plant regeneration and in vitro cormlet development. C. scepusiensis and the rest of cultures (organogenic vs. embryogenic) differed at the biochemical level: C. scepusiensis cultures had higher (yet still low) enzymatic antioxidant (catalase, peroxidase) activities. With respect to catalase isoenzyme patterns, C. banaticus was different from the rest of cultures, demonstrating its distinct taxonomical position. Besides germplasm preservation use of the present cultures, they have a potential biotechnological value.</jats:p>
eISSN 1898-0295 ; ISSN 0001-5296
LOSKUTOV (1999), Optimization of in vitro conditions for stigma - like - structure production from half - ovary explants of Crocus sativus In vitro Cellular and Developmental Plant, Biology, 200. ; MOSOLYGÓ (2016), Molecular genetic evidence for allotetraploid speciation in the genus Crocus, null, 258. ; WEIDERT (2010), of superoxide dismutase catalase and glutathione peroxidase in cultured cells and tissue, Measurement Nature Protocols, 5. ; SHARMA (2008), In vitro cormlet development in Crocus sativus, Biologia Plantarum, 709. ; ASCOUGH (2009), Micropropagation of a and, review Plant Cell Tissue Organ Culture, 1. ; FERNÁNDEZ (2006), Anticancer properties of saffron Crocus sativus In Lead molecules from natural products Amsterdam The, null, 30, 313. ; BLAZQUEZ (2009), embryogenesis in saffron ( Crocus sativus Histological differentiation and implication of some components of the antioxidant enzymatic system and, Plant Cell Tissue Organ Culture, 97, 49. ; SIVANESAN (2012), SON JANA Secondary somatic embryogenesis in Crocus vernus of, Propagation Ornamental Plants, 12, 163. ; PĂTROESCU (2000), Natural transborder parks : the direction of biodiversity preservation in In eds Implementing ecological integrity restoring regional and global environmental and human The, Romania health Acad, 101. ; DEMETER (2014), Osmotic stress responses of individual white oak section subgenus genotypes cultured in vitro of, Journal Plant Physiology, 171. ; KIRÁLY (2007), magyarországi edényes flóra veszélyeztetett fajai / Red list of the vascular flora in Print in Hungarian abstract available in, English, 13. ; MURASHIGE (1962), revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiologia Plantarum, 15, 473. ; DEMETER (2010), embryogenesis and regeneration from shoot primordia of Crocus heuffelianus and, Plant Cell Tissue Organ Culture, 349. ; FERNÁNDEZ (2011), The world saffron and Crocus collection : strategies for establishment management characterisation utilisation and, Genetic Resources Crop Evolution, 125. ; GAMBORG (1968), Nutrient requirements of suspension cultures of soybean root cells, Experimental Cell Research, 151. ; MÁTHÉ (2012), The plant tissue culture collection at Department of University of, Botany Acta Biologica, 179. ; Negbi (1999), PLESSNER In vitro propagation and secondary metabolite production in Crocus sativus In ed Medicinal and aromatic plants - industrial profiles Academic Publishers Amsterdam The, null, 137. ; DEVI (2014), Direct somatic embryogenesis with high frequency plantlet regeneration and successive cormlet production in saffron ( Crocus sativus, PS South African Journal of Botany, 207. ; SHARIFI (2010), Changes of antioxidant enzyme activities and isoenzyme profiles during in vitro shoot formation in saffron ( Crocus sativus, Acta Biologica Hungarica, 61. ; BHAGYALAKSHMI (1999), Factors influencing direct shoot regeneration from ovary explants of saffron and, Plant Cell Tissue Organ Culture, 205. ; WERYSZKO (2011), Structure of the floral parts of Crocus vernus, Acta Agrobotanica, 64, 35. ; KARAMIAN (2001), Plantlet regeneration from protoplast - derived embryogenic calli of Crocus cancellatus and, Plant Cell Tissue Organ Culture, 65. ; GANTAIT (null), In vitro regeneration of high value spice Crocus sativus a concise appraisal of Applied Research on Medicinal and Aromatic Plants, Journal, 2015. ; EBRAHIMZADEH (2000), In vitro production of floral buds and stigma - like structures on floral organs of Crocus sativus of, Pakistan Journal Botany, 141. ; ESCRIBANO (1999), Isolation and cytotoxic properties of a novel glycoconjugate from corms of saffron plant ( Crocus sativus, Biochimica et Biophysica Acta, 217. ; SIVANESAN (2014), JANA In vitro shoot regeneration and microcorm development in Crocus vernus of, Pakistan Journal Botany, 693. ; KARAMIAN (2010), Study on changes in protein patterns and phenolic contents during somatic embryogenesis in Crocus sativus, Acta Horticulturae, 850. ; GARDA (2016), Microcystin - LR induces mitotic spindle assembly disorders in Vicia faba by protein phosphatase inhibition and not reactive oxygen species induction of, Journal Plant Physiology, 199. ; ZEYBEK (2012), Improved in vitro micropropagation methods with adventitious corms and roots for endangered saffron, Central European Journal of Biology, 138. ; BRADFORD (1976), rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein - dye binding, Analytical Biochemistry, 72.