Details

Title

Effects of specific inhibitors on the gene expression of a digestive trypsin in Pieris brassicae L. (Lepidoptera: Pieridae)

Journal title

Journal of Plant Protection Research

Yearbook

2018

Numer

No 1

Publication authors

Keywords

gene expression ; Pieris brassicae ; synthetic inhibitors ; trypsin

Divisions of PAS

Nauki Biologiczne i Rolnicze

Abstract

Protein digestion in insects relies on several groups of proteases, among which trypsin plays a prominent role. In the current study, larvae of Pieris brassicae L. were fed radish leaves treated with 1 mM concentrations of three specific inhibitors of trypsin: AEBSF.HCl [4-(2- -aminoethyl)-benzenesulfonyl fluoride, monohydrochloride], TLCK (N-a-tosyl-l-lysine chloromethyl ketone) and SBTI (Soybean Trypsin Inhibitor) to find their potential effects on gene expression of trypsin. Initially, RT-PCR analysis revealed a gene of 748 bp responsible for synthesizing the digestive trypsin in P. brassicae larvae. Also, qRT-PCR data indicated a statistically greater expression of trypsin gene in the larvae fed 1 mM concentrations of AEBSF.HCl, TLCK and SBTI than the control. Results of the current study indicated that synthetic inhibitors can not only negatively affect the gene expression of P. brassicae trypsin, but also the insect can activate a compensatory mechanism against interruption of protein digestion by inducing more expression of the gene and producing more trypsin into the midgut lumen.

Publisher

Polish Society of Plant Protection ; Committee of Plant Protection PAS ; Institute of Plant Protection – National Research Institute

Date

2018.03.30

Type

Artykuł

Identifier

eISSN 1899–007X ; ISSN 1427–4345

DOI

10.24425/119112

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