Details

Title

Production, characterization and iron binding affinity of hydroxamate siderophores from rhizosphere associated fluorescent Pseudomonas

Journal title

Journal of Plant Protection Research

Yearbook

2018

Numer

No 1

Publication authors

Keywords

siderophore ; hydroxamate ; fluorescent Pseudomonas ; succinate medium ; iron-binding ; mutation

Divisions of PAS

Nauki Biologiczne i Rolnicze

Abstract

Fluorescent Pseudomonas (FP) is a major group of plant growth promoting rhizobacteria and a well-known synthesizer of siderophores, which imparts a selective advantage on rhizosphere competence and their biocontrol traits. The present study was aimed at examining the factors affecting the production of siderophores and their potential biocontrol traits. Sixteen FP isolates were shortlisted based on their siderophore-producing ability in chrome azural S medium. The isolates were checked for variations in siderophore production under varying incubation times, temperatures, pH, iron (Fe3+) concentrations and mutagens. In addition, the iron binding affinity of siderophores, mycelial inhibition assay and plant growth promotion traits were assessed. Results showed that the siderophore production was highly influenced by the time of incubation, changes in pH, temperature and iron concentration. Chemical characterization showed that the produced siderophores were hydroxamates. Maximum siderophore production was observed at pH 7 whereas UV and EtBr exposure invariably suppressed siderophore production drastically in all isolates. All FPs from maize rhizosphere showed excellent siderophore production which could be due to the competence in strategy-II of the plant rhizosphere and significant growth inhibition on Fusarium oxysporum. Our results suggest the inclination of siderophores to iron, in terms of various criteria affecting production and the possible role of environmental mutations that affect the natural iron harvesting mechanism.

Publisher

Polish Society of Plant Protection ; Committee of Plant Protection PAS ; Institute of Plant Protection – National Research Institute

Date

2018.03.30

Type

Artykuł

Identifier

eISSN 1899–007X ; ISSN 1427–4345

DOI

10.24425/119116

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