Details

Title

Production and characterization of egg yolk antibody (IgY) against recombinant VP8-S2 antigen

Journal title

Polish Journal of Veterinary Sciences

Yearbook

2016

Numer

No 2

Publication authors

Divisions of PAS

Nauki Biologiczne i Rolnicze

Abstract

<jats:title>Abstract</jats:title><jats:p>Bovine Rotavirus and Bovine Coronavirus are the most important causes of diarrhea in newborn calves and in some other species such as pigs and sheep. VP8 subunit of rotavirus is the major determinant of the viral infectivity and neutralization. Spike glycoprotein of coronavirus is responsible for induction of neutralizing antibody response. Studies showed that immunoglobulin of egg yolk (IgY) from immunized hens has been identified to be a convenient source for specific antibodies for using in immunotherapy and immunodiagnostic to limit the infections.</jats:p><jats:p>In this study, chimeric VP8-S2 gene was designed using by computational techniques. The chimeric VP8-S2 gene was cloned and sub-cloned into pGH and pET32a (+) vectors. Then, recombinant pET32a-VP8-S2 vector was transferred into</jats:p><jats:p>We obtained anti-VP8-S2 IgY by immunizing hens with the recombinant VP8-S2 protein. The anti-VP8-S2 IgY was showed to bind specifically to the chimeric VP8-S2 protein by dot-blotting, Western-blotting analyses and indirect ELISA. The result of this study indicated that such construction can be useful to investigate as candidates for development of detection methods for simultaneous diagnosis of both infections. Specific IgY against the recombinant VP8-S2 could be recommended as a candidate for passive immunization against bovine rotavirus and bovine coronavirus.</jats:p>

Publisher

University of Warmia and Mazury in Olsztyn ; Polish Academy of Sciences Committee of Veterinary Sciences

Date

2016

Identifier

ISSN 1505-1773

DOI

10.1515/pjvs-2016-0034

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