Details

Title

The effects of freezing on long-term storage of canine erythrocytes

Journal title

Polish Journal of Veterinary Sciences

Yearbook

2016

Numer

No 2

Publication authors

Divisions of PAS

Nauki Biologiczne i Rolnicze

Abstract

<jats:title>Abstract</jats:title><jats:p>Human medicine studies have so far demonstrated that erythrocytes may be preserved and stored at low temperatures for decades retaining their metabolic and biochemical properties. However, detailed studies regarding this problem are not yet available in veterinary medicine. Therefore, the objective of the current study was to investigate time-dependent effects of long-term frozen storage of canine red blood cells.</jats:p><jats:p>Twelve healthy adult dogs meeting the criteria for blood transfusion were used in the study. Whole blood samples (450 ± 45 ml) collected from each dog were centrifuged by a cryogenic microcentrifuge and packed RBC suspensions were obtained. The samples were prewashed three times in 0.9% NaCl solution and were allocated into three groups to be evaluated at three different time points (day 0 and month 4 and 6). The samples to be frozen were subjected to glycerolization and then stored at −80°C for 4 and 6-month periods. At the end of this period the packed RBC samples were thawed, centrifuged and then washed in a consecutive series of dextrose solutions. 2,3-Diphosphoglycerate (2,3-DPG), Adenosin triphosphate (ATP), supernatant hemoglobin (SupHb), sodium (Na</jats:p>

Publisher

University of Warmia and Mazury in Olsztyn ; Polish Academy of Sciences Committee of Veterinary Sciences

Date

2016

Identifier

ISSN 1505-1773

DOI

10.1515/pjvs-2016-0050

×