Details

Title

Use of Fluorescence Quantitative Polymerase Chain Reaction (PCR) for the Detection of Escherichia coli Adhesion to Pig Intestinal Epithelial Cells

Journal title

Polish Journal of Veterinary Sciences

Yearbook

2016

Numer

No 3

Publication authors

Divisions of PAS

Nauki Biologiczne i Rolnicze

Publisher

University of Warmia and Mazury in Olsztyn ; Polish Academy of Sciences Committee of Veterinary Sciences

Date

2016

Identifier

ISSN 1505-1773

References

Wall (2002), Quantitative reverse transcription - polymerase chain reaction comparison of primer - dropping , competitive , and real - time RT - PCRs, Anal Biochem, 300. ; Hohmann (1975), Adherence of enteropathogenicEscherichia colito intestinal epithelium in vivo, Infect Immun, 12, 866. ; Schmittgen (2001), Real - time quantitative PCR, Methods, 25, 383, doi.org/10.1006/meth.2001.1260 ; Nataro (1998), DiarrheagenicEscherichia coli, Clin Microbiol Rev, 11, 142. ; Imberechts (1994), Prevalence of fimbriae onEscherichia coliisolated from pigs with oedema disease or post - weaning diarrhoea, Vet Microbiol, 40, 107, doi.org/10.1016/0378-1135(94)90111-2 ; Melkebeek (2012), Targeting aminopeptidase a newly identified receptor for ac fimbriae , enhances the intestinal mucosal immune response, Mucosal Immunol, 5, 635, doi.org/10.1038/mi.2012.37 ; Wu (2007), Polymorphisms of the FUT gene locus in post - weaning Sutai breed piglet and resistance to fimbrial Escherichia coli invitro Chinese of, Journal Preventive Veterinary Medicine, 18, 307. ; Turner (2006), Weapons of mass destruction : virulence factors of the global killer enterotoxigenicEscherichia coli, FEMS Microbiol Lett, 263. ; Atshan (2012), - Prevalence of adhesion and regulation of biofilm - related genes in different clones of staphylococcus aureus, J Biomed Biotechnol, 976972. ; Wu (2014), Attak Experiment and Phenotype Analysis of Meishan Piglets byE coliF, Strain Acta Veterinaria Et Zootechnica Sinica, 18, 1608. ; Zhang (2010), Radix Isatis Polysaccharides to Inhibit Adhesion of PathogenicE colito Cell, J Microbiol, 30, 61. ; Li (2010), Effects of HMGN on bacterial adhesion to Hela Cells Medical of West China, Journal, 22, 788. ; Zhang (2007), Prevalence of virulence genes inEscherichia colistrains recently isolated from young pigs with diarrhea in the US, Vet Microbiol, 123. ; Tawakoli (2013), Comparison of different live / dead stainings for detection and quantification of adherent microorganisms in the initial oral biofilm, Clin Oral Investig, 17, 841, doi.org/10.1007/s00784-012-0792-3 ; Livak (2001), Analysis of relative gene expression data using real - time quantitative PCR and the - ΔΔCT Method, Methods, 25, 402, doi.org/10.1006/meth.2001.1262 ; Yu (2012), Heat - labile enterotoxin enhances etec adherence to porcine small intestine epithelial cell line IPEC - Chinese of, Journal Animal Infectious Diseases, 20, 29. ; Shi (2015), Relationship of surface properties of silicone rubber with bacteria adhesion, J Third Mil Med Univ, 37, 1921. ; Bertschinger (1972), Association ofEscherichia coliwith the small intestinal epithelium Comparison of enteropathogenic and nonenteropathogenic porcine strains in pigs, Infect Immun, 5, 595. ; Candela (2008), Interaction of probiotic Lactobacillus and Bifidobacterium strains with human intestinal epithelial cells : adhesion properties , competition against enteropathogens and modulation of IL - production, Int J Food Microbiol, 8, 125.

DOI

10.1515/pjvs-2016-0077

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