Details

Title

Gut microbiota isolated from the European pond turtle (Emys orbicularis) and its antimicrobial resistance

Journal title

Polish Journal of Veterinary Sciences

Yearbook

2016

Numer

No 4

Publication authors

Divisions of PAS

Nauki Biologiczne i Rolnicze

Abstract

<jats:title>Abstract</jats:title><jats:p>The aim of the study was to isolate cultivable gut microbiota from European pond turtles kept at the Lithuanian Zoo and to determine antimicrobial resistance of the isolates. The study subjects included 8 elderly turtles living at the Lithuanian Zoo for about 50 years as well as their offspring – 24 young individuals (1-2 years old) that were hatched at the same zoo. Animals were not exposed by treatment with antimicrobials during the last 3 years.</jats:p><jats:p>Gut samples were taken from the cloaca and inoculated onto universal media. Isolates then were identified using sequence analysis of 16S rRNA.</jats:p><jats:p>The antimicrobial susceptibility testing was performed using the agar diffusion method according to Kirby-Bauer. Clinical breakpoints according to CLSI whenever possible, were used for interpretation of susceptibility. Bacterial isolates resistant to at least three antimicrobials of different classes were treated as multi-resistant.</jats:p><jats:p>Fifty-two bacterial isolates were obtained and identified from turtle gut samples. The most prevalent genera included</jats:p>

Publisher

University of Warmia and Mazury in Olsztyn ; Polish Academy of Sciences Committee of Veterinary Sciences

Date

2016

Identifier

ISSN 1505-1773

DOI

10.1515/pjvs-2016-0091

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