Details

Title

In vitro fertilizing potential of urethral and epididymal spermatozoa collected from domestic cats (Felis catus)

Journal title

Polish Journal of Veterinary Sciences

Yearbook

2017

Numer

No 1

Publication authors

Divisions of PAS

Nauki Biologiczne i Rolnicze

Abstract

<jats:title>Abstract</jats:title><jats:p>The aim of this study was to provide a comparative analysis of in vitro fertilizing potential of frozen-thawed urethral and epididymal feline spermatozoa. Both types of semen were collected from 7 cats and cryopreserved in liquid nitrogen. To perform in vitro fertilization, both urethral and epididymal samples from the same individual were thawed and spermatozoa were co-incubated with in vitro matured cat oocytes. Obtained embryos were cultured in vitro for 7 days in a commercial medium. Cleavage rate, morula rate and blastocyst rate were calculated. Experiment was run in 10 replicates. The examined parameters showed no significant differences between urethral and epididymal spermatozoa (p&gt;0.05). Cleavage rate and embryo’s development were highly variable between replicates, even for the different sperm samples collected from one individual. There was no significant correlation between fertilizing capacity of two types of spermatozoa collected from the same male. In this study we confirmed that cryopreserved urethral spermatozoa have equally good fertilizing potential as epididymal ones, and both can be successfully used for in vitro fertilization in cats with the use of commercial medium.</jats:p>

Publisher

University of Warmia and Mazury in Olsztyn ; Polish Academy of Sciences Committee of Veterinary Sciences

Date

2017

Identifier

ISSN 1505-1773

DOI

10.1515/pjvs-2017-0003

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