Morphometric abnormalities in the spleen of the progeny of mice fed epigallocatechin during gestation and nursing

Journal title

Polish Journal of Veterinary Sciences




No 1

Publication authors

Divisions of PAS

Nauki Biologiczne i Rolnicze


<jats:title>Abstract</jats:title><jats:p>It is very difficult to cure pregnant females suffering from infections, because of the risk which might occur during treatment by several, even herbal, medications. Many of these substances, among them extracts from plants, have antimicrobial, anti-inflammatory and immunostimulatory properties owing to their polyphenols content, but also may reveal unwanted effects on the fetal development because of their anti-angiogenic properties. The aim of the present study was to elucidate whether daily feeding pregnant and nursing mice 0.2 mg/kg epigallocatechin (EGC), previously recognized as angiogenesis inhibitor, may lead to abnormalities in morphology of spleen and in some parameters of immune function of their adult, 6-week old progeny. Morphometry of EGC offspring spleens revealed lower number of lymphatic nodules and their larger diameter than those found in the control offspring. Cellularity of spleens was lower in EGC offspring than in the controls. Cytometric analysis showed that this decline concerns lymphocytes with CD335 (p&lt;0.001), CD19 (p&lt;0.01) and CD4 (p&lt;0.05) markers. No differences were observed in the humoral response to the immunization with SRBC, and in the proliferative response of splenocytes to mitogens PHA, ConA and LPS.</jats:p>


University of Warmia and Mazury in Olsztyn ; Polish Academy of Sciences Committee of Veterinary Sciences




ISSN 1505-1773