Details

Title

DNA Stability Contrasts with Chromosome Variability in Allium Fistulosum Calli

Journal title

Acta Biologica Cracoviensia s. Botanica

Yearbook

2014

Numer

No 1

Publication authors

Divisions of PAS

Nauki Biologiczne i Rolnicze

Abstract

Abstract RAPD analysis was applied to assess the degree of DNA polymorphism in A. fistulosum calli of high chromosomal instability. Nineteen of 24 randomly selected RAPD primers revealed scorable polymorphism between calli and seeds (reference material). Polymorphic band frequency was 55/237 in seeds and 36/233 in calli; variability on the DNA level was thus lower in calli than in seeds (15.4% vs. 23.2% of band positions). UPGMA analysis of Jaccard's coefficients confirmed the genetic similarity of the analyzed cultures. The most distinctive DNA changes in calli involved coincident loss of original bands or the appearance of novel bands. Seven such changes (4 losses, 3 gains) were observed. Our results suggest that changes on the chromosomal level and on the DNA level occurred independently of each other and that different callus lines underwent similar genetic changes during culture, presumably due to strong selection pressure effected by standard in vitro conditions.

Publisher

Biological Commission of the Polish Academy of Sciences – Cracow Branch

Date

2014

Identifier

eISSN 1898-0295 ; ISSN 0001-5296

DOI

10.2478/abcsb-2014-0005

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