Details

Title

Plant Regeneration Via Organogenesis and Somatic Embryogenesis in Verbascum Sinuatum L.

Journal title

Acta Biologica Cracoviensia s. Botanica

Yearbook

2014

Numer

No 1

Publication authors

Divisions of PAS

Nauki Biologiczne i Rolnicze

Abstract

Abstract The genus Verbascum L. belongs to the family Scrophulariaceae and its members are used as medicinal herbs in traditional medicines worldwide. In this study we achieved plant regeneration in Verbascum sinuatum L. via organogenesis and somatic embryogenesis by culture of mature embryos. Embryogenic and nonembryogenic calli were induced from mature embryos on Murashige and Skoog (MS) medium supplemented with different concentrations of benzyl adenine (BA) and a-naphthalene acetic acid (NAA) (but not for 1.5 and 3 mg l−1 NAA). For multiplication of somatic embryoids and differentiation of shoot buds, yellow and friable embryonic calli were transferred to MS medium containing 30 g/l sucrose, 0.5 mg l−1 charcoal and 0.1 or 1 mg l−1 2,4-dichlorophenoxy acetic acid (2,4-D) or to MS medium containing 60 g l−1 sucrose, 50 mg l−1 casein hydrolysate (CH), 0.5 mg l−1 kinetin (Kin), 5 mg l−1 2,4-D and 0.5 mg l−1 charcoal. Shoot multiplication and plantlet regeneration were achieved by transferring shoot buds to MS medium supplemented with 1 mg l−1 BA or Kin.

Publisher

Biological Commission of the Polish Academy of Sciences – Cracow Branch

Date

2014

Identifier

eISSN 1898-0295 ; ISSN 0001-5296

DOI

10.2478/abcsb-2014-0010

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