Details

Title

Embedding in Filter-Sterilized Alginate Enhances Brassica Oleracea L. Protoplast Culture

Journal title

Acta Biologica Cracoviensia s. Botanica

Yearbook

2014

Numer

No 2

Publication authors

Divisions of PAS

Nauki Biologiczne i Rolnicze

Abstract

Abstract The influence of sodium alginate sterilization on the viability and mitotic activity of embedded protoplasts was studied in protoplasts of Brassica oleracea subsp. alba and rubra isolated from hypocotyl tissue and leaves of seedlings or plants grown in vitro. Both leaf and hypocotyl-derived protoplasts were more viable and divided more frequently when embedded in filtrated alginate. Division frequency was highest in cv. Reball F1 and the mitotic activity of its protoplasts was three times higher when embedded in filtrated alginate (36.1 ± 6.8%) than when cultured in autoclaved alginate (10.9 ± 5.0%). Protoplast-derived calli colonies were transferred to solid regeneration media and plants of all tested accessions were obtained.

Publisher

Biological Commission of the Polish Academy of Sciences – Cracow Branch

Date

2015[2015.01.01 AD - 2015.12.31 AD]

Identifier

eISSN 1898-0295 ; ISSN 0001-5296

DOI

10.2478/abcsb-2014-0018

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