Sucrose and Cytokinin Interactions in Relation to Ethylene and Abscisic Acid Production in the Regulation of Morphogenesis in Pelargonium × hortorum L.H. Bailey In Vitro

Journal title

Acta Biologica Cracoviensia s. Botanica




No 1

Publication authors

Divisions of PAS

Nauki Biologiczne i Rolnicze


Abstract The aim of the study was to determine the effect of exogenous sucrose and cytokinin on ethylene production and responsiveness in relation to the shoot formation of Pelargonium × hortorum ‘Bergpalais’ in vitro. Increasing the concentration of sucrose from 15 to 40 g L−1 in medium containing meta-topolin (mT) resulted in a two-fold decrease in the number of shoots and leaves as well as a reduction in ethylene production. The addition of ethylene synthesis inhibitor (AVG) to mT-medium significantly reduced the ethylene production and the shoot growth, but it had no significant influence on the shoot formation. The mT-induced shoot formation was, however, significantly reduced in the presence of ethylene action inhibitor (AgNO3), in a manner dependent on sucrose levels. At the end of the subculture period, increased sucrose concentrations (15–40 g L−1) in the presence of mT and AgNO3 resulted in a 3.7-fold increase in ethylene emission. At the same time, the supply of sucrose caused a 2.8-fold increase in the level of endogenous abscisic acid (ABA). Our results may suggest that the inhibitory effect of high sucrose concentration (30 and 40 g L−1) may depend on its influence on ethylene sensitivity. It also suggests that sucrose-regulation of the shoot formation of Pelargonium in vitro is mediated by ABA.


Biological Commission of the Polish Academy of Sciences – Cracow Branch


2015[2015.01.01 AD - 2015.12.31 AD]


ISSN 0001-5296 ; eISSN 1898-0295


CiardiJ (2001), Regulation of ethylene - mediated responses at the level of the receptor, Annals of Botany, 88, 813, ; Philosoph (1985), Carbohydrates stimulate ethylene production in tobacco leaf discs II Sites of stimulation in the ethylene biosynthesis pathway, Plant Physiology, 78, 139, ; BeaudoinN (2000), Interactions between abscisic acid and ethylene signaling cascade, The Plant Cell, 12, 1103, ; MurashigeT (1962), A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiologia Plantarum, 15, 473, ; RognoniS (2007), Sugar effect on early seedling development inArabidopsis, Plant Growth Regulation, 52, 217, ; KobayashiH (2003), Relationship between ethylene evolution and sucrose content in excised leaf blades of rice, Plant Production Science, 3, 398, ; LeonP (2003), Sugar and hormone connections, Trends in Plant Science, 8, 110, ; GibsonSI (2001), The sugar - intensitive sis mutant ofArabidopsisis allelic toctr Biochemical and Biophysical Research, Communication, 1. ; WojtaniaA (2010), Effect ofmeta - topolin on in vitro propagation ofPelargonium hortorumandPelargonium hederaefolium cultivars, Acta Societatis Botanicorum Poloniae, 79, 101, ; GibsonSI (2005), Control of plant development and gene expression by sugar signaling, Current Opinion in Plant Biology, 8, 93, ; YanagisawaS (2003), Differential regulation of EIN stability by glucose and ethylene signaling in plants, Nature, 425. ; DobrevPI (2002), Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed - mode solid - phase extraction, Journal of Chromatography A, 950. ; YangSF (1984), Ethylene biosynthesis and its regulation in higher plants, Annual Review of Plant Physiology, 35, 155, ; MeirS (1989), Carbohydrates stimulate ethylene production in tobacco leaf discs III Stimulation of enzymatic hydrolysis of indole - - acetyl - L - alanine, Plant Physiology, 90, 1246, ; StefancicM (2007), The levels of IAA , IAAsp and some phenolics in cherry root - stock GiSelA leafy cuttings pretreated with IAA and IBA, Scientia Horticulturae, 112, 399, ; SmeekensS (2010), Sugar signals and molecular network controlling plant growth, Current Opinion in Plant Biology, 13, 274, ; MagdalitaPM (1997), Effect of ethylene and culture environment on development of papaya nodal cultures ,, Plant Cell Tissue and Organ Culture, 49, 93, ; WojtaniaA (2012), - LesiakE Ethylene and cytokinin interaction in the morphogenesis ofPelargonium hortorumL Bailey in vitro, Acta Physiologiae Plantarum, 34, 2407, ; HansonJ (2009), Sugar perception and signaling an update, Current Opinion in Plant Biology, 562, ; SoenoK (2010), Auxin biosynthesis inhibitors , identified by a genomics - based approach , provide insights into auxin biosynthesis, Plant Cell Physiology, 51, 524, ; GazzariniS (2001), Genetic interactions between ABA , ethylene and sugar signaling pathways, Current Opinion in Plant Biology, 4, 387, ; PodwyszyńskaM (1998), Effect of inhibitors of ethylene biosynthesis and action , as well as calcium and magnesium on rose shoot rooting , shoot - tip necrosis and leaf senescence in vitro, Acta Physiologiae Plantarum, 20, 91, ; AyyappanV (2006), Effect of ethylene inhibitors on in vitro shoot multiplication and their impact on ethylene production in cucumber ( Cucumis sativusL ), Research Journal of Botany, 1, 110, ; GhassemianM (2000), Regulation of abscisic acid signaling by ethylene response pathway inArabidopsis, Plant Cell, 12, 1117, ; ChangC (2004), Ethylene biology More than a gas, Plant Physiology, 136. ; GabryszewskaE (2010), The effects of glucose and growth regulators on the organogenesis ofPaeonia lactifloraPall in vitro Journal of Fruit and Ornamental Plant, Research, 18, 309. ; HorbowiczM (2011), Effects of α - aminooxyacetic acid on the level of polyamines , anthocyanins and photosynthetic pigments in seedlings of common buckwheat ( Fagopyrum esculentumMOENCH ), Acta Societatis Botanicorum Poloniae, 2, 99,