TitleElicitation of Anthocyanin Production in Roots of Kalanchoe blossfeldiana by Methyl Jasmonate
Journal titleActa Biologica Cracoviensia s. Botanica
Divisions of PASNauki Biologiczne i Rolnicze
AbstractAbstract The influence of methyl jasmonate on anthocyanin accumulation in roots of Kalanchoe blossfediana plants was studied. Methyl jasmonate (JA-Me), at a concentration of 5.0 to 40.0 mg.l−1, substantially increased anthocyanin accumulation in roots of intact plants, when it was applied as a solution under natural light conditions. The production of anthocyanin depended on the concentration of methyl jasmonate and the age of the plant. The stimulatory effect was higher in older plants of K. blossfeldiana than in younger ones. When leaves were removed methyl jasmonate slightly stimulated anthocyanin accumulation compared with intact plants. The obtained results indicate that leaves are necessary for the anthocyanin accumulation in the roots. In isolated roots methyl jasmonate did not affect the accumulation of anthocyanins in light conditions. Seven anthocyanins were documented in the roots of control plants and 8 anthocyanins in the roots of JA-Me treated ones. JA-Me increased the level of anthocyanins in roots of old K. blossfeldiana plants 6.8, 6.0 and 3.6-folds, after 4, 8 and 14-days of treatment, respectively.
PublisherBiological Commission of the Polish Academy of Sciences – Cracow Branch
Date2015[2015.01.01 AD - 2015.12.31 AD]
IdentifiereISSN 1898-0295 ; ISSN 0001-5296
ReferencesCreelmanRA (1997), Biosynthesis and action of jasmonates in plants, Annual Review of Plant Physiology and Plant Molecular Biology, 48, 355, doi.org/10.1146/annurev.arplant.48.1.355 ; IshikuraN (1962), Anthocyanins in red roots of a radish Studies on anthocyanins , XXXVIThe Botanical Magazine, Tokyo, 75, 28, doi.org/10.15281/jplantres1887.75.28 ; Chalker (1999), Environmental significance of anthocyanin in plants stress responses, Photochemistry and Photobiology, 70, 1, doi.org/10.1111/j.1751-1097.1999.tb01944.x ; LewisCE (1998), Light regulation of anthocyanin , flavonoid and phenolic acid biosynthesis in potato minitubers in vitro, Australian Journal of Plant Physiology, 25, 915, doi.org/10.1071/PP98112 ; BarnesJ (2011), Structural characterization of cyanidin diglucoside and pelargonidin diglucoside anthocyanins : Multi - dimensional fragmentation pathways using high performance liquid chromatography - electrospray ionization - ion trap - time of flight mass spectrometry, International Journal of Mass Spectrometry, 3. ; JuZY (2003), Effects of solvent and temperature on pressurized liquid extraction of anthocyanins and total phenolics from dried red grape skin, Journal of Agricultural and Food Chemistry, 51, 5207, doi.org/10.1021/jf0302106 ; DaoTTH (2011), Chalcone synthase and its functions in plant resistance, Phytochemistry Reviews, 10, 397, doi.org/10.1007/s11101-011-9211-7 ; IslamMS (2005), Artificial shading and temperature influence on anthocyanin compositions in sweet potato leaves, HortScience, 40, 176. ; ZhangW (2002), Integration of jasmonic acid and light irradiation for enhancement of anthocyanin biosynthesis inVitis viniferasuspension cultures, Plant Science, 162, 459, doi.org/10.1016/S0168-9452(01)00586-6 ; ThakurM (1978), Anthocyanin pigmentation in roots ofImpatiensspecies, Canadian Journal of Botany, 56, 2898, doi.org/10.1139/b78-347 ; TakosAM (2006), Light - induced expression of a MYB gene regulates anthocyanin biosynthesis in red apples, Plant Physiology, 142, 1216, doi.org/10.1104/pp.106.088104 ; MancinelliAL (1988), Anthocyanin production in Chl - rich and Chl - poor seedlings, Plant Physiology, 86, 652, doi.org/10.1104/pp.86.3.652 ; SaniewskiM (2003), Methyl jasmonate stimulates the formation and the accumulation of anthocyanin inKalanchoe blossfeldiana, Acta Physiologiae Plantarum, 25, 143, doi.org/10.1007/s11738-003-0047-7 ; TselasSK (1979), Anthocyanin formation in maize roots, Plant Science Letters, 16, 81, doi.org/10.1016/0304-4211(79)90011-7 ; PlataN (2003), Effect of methyl jasmonate and p - coumaric acid on anthocyanin composition in a sweet potato cell suspension culture, Biochemical Engineering Journal, 14, 171, doi.org/10.1016/S1369-703X(02)00218-8 ; RichardS (2000), Induction of chalcone synthase expression in white spruce by wounding and jasmonate, Plant and Cell Physiology, 41, 982, doi.org/10.1093/pcp/pcd017 ; NishiyamaY (2004), Effect of medium composition on the production of anthocyanins by hairy root cultures ofIpomoea batatas, Plant Biotechnology, 21, 411, doi.org/10.5511/plantbiotechnology.21.411 ; KimChH (1992), Enhanced anthocyanin production in hairy roots culture ofDaucus carotaby fungal elicitors, HortScience, 27, 694. ; ZhouY (2004), Effect of light on anthocyanin levels in submerged , harvested cranberry fruit, Journal of Biomedicine and Biotechnology, 5, 259, doi.org/10.1155/S1110724304403027 ; FeysBJF (1994), Arabidopsismutants selected for resistance to the phytotoxin coronatine are male sterile , insensitive to methyl jasmonate , and resistant to a bacterial pathogen, Plant Cell, 6, 751, doi.org/10.1105/tpc.6.5.751 ; NeylandM (1963), Formation of anthocyanin in leaves ofKalanchoe blossfeldiana a photoperiodic response, Plant Physiology, 38, 447, doi.org/10.1104/pp.38.4.447 ; CallebautA (1997), Stability of anthocyanin composition inAjuga reptanscallus and cell suspension cultures, Plant Cell Tissue and Organ Culture, 50, 195, doi.org/10.1023/A:1005905421340 ; UbiBE (2004), External stimulation of anthocyanin biosynthesis in apple fruit Agriculture and, Journal Food Environment, 2, 65. ; NemecS (1973), Phenolics in the strawberry root, Annals of Botany, 72, 1311. ; RaoAS (1990), Root flavonoids, The Botanical Review, 56, 1, doi.org/10.1007/BF02858531 ; KammererD (2003), Detection of peonidin and pelargonidin glycosides in black carrots ( Daucus carotassp sativusvar atrorubensAlef ) by high - performance liquid chromatography / electrospray ionization mass spectrometry, Rapid Communications in Mass Spectrometry, 17, 2407, doi.org/10.1002/rcm.1212 ; MerzlyakMN (2002), Patterns of pigment changes in apple fruits during adaptation to high sunlight and sunscald development, Plant Physiology and Biochemistry, 40, 679, doi.org/10.1016/S0981-9428(02)01408-0 ; NielsenAH (2005), Flavonoids in flowers of Kalanchoe blossfeldianavarieties, Phytochemistry, 66, 2829, doi.org/10.1016/j.phytochem.2005.09.041 ; NohB (1998), Anion channels and the stimulation of anthocyanin accumulation by blue light inArabidopsisseedlings, Plant Physiology, 116, 503, doi.org/10.1104/pp.116.2.503 ; HarborneJB (1963), Plant polyphenols IX The glycosidic pattern of anthocyanin pigments, Phytochemistry, 2, 85, doi.org/10.1016/S0031-9422(00)88020-6 ; FangY (1999), Effects of exogenous methyl jasmonate in elicited anthocyanin - production cell cultures of ohelo ( Vaccinium pahalae ) In Vitro Cellular and Developmental Biology, Plant, 35, 106. ; CurtinCH (2003), Manipulating anthocyanin composition inVitis viniferasuspension cultures by elicitation with jasmonic acid and light irradiation, Biotechnology Letters, 25, 1131, doi.org/10.1023/A:1024556825544 ; SolangaarachchiSM (2001), Anthocyanin pigmentation in the adventitious roots ofMetrosideros excelsa Myrtaceae ), New Zealand Journal of Botany, 39, 161, doi.org/10.1080/0028825X.2001.9512724 ; PageM (2012), The influence of ascorbate on anthocyanin accumulation during high light acclimation inArabidopsis thaliana : further evidence for redox control of anthocyanin synthesis, Plant Cell and Environment, 35, 388, doi.org/10.1111/j.1365-3040.2011.02369.x ; BalsaC (1979), Photoperiodic control of phenolic metabolism inKalanchoe blossfeldiana, Phytochemistry, 18, 1159, doi.org/10.1016/0031-9422(79)80126-0 ; ShimizuY (2010), Methyl jasmonate induces anthocyanin accumulation inGynura bicolorcultured roots -, In Vitro Cellular Developmental Biology Plant, 46, 460, doi.org/10.1007/s11627-010-9294-7 ; TamariG (1995), Methyl jasmonate induces pigmentation and flavonoid gene expression in petunia corollas : A possible role in wound response, Physiologia Plantarum, 94, 45, doi.org/10.1034/j.1399-3054.1995.940107.x ; CheongJ (2003), Methyl jasmonate as a vital substance in plants, Trends in Genetics, 19, 409, doi.org/10.1016/S0168-9525(03)00138-0 ; YamakawaT (1983), Production of anthocyanins byVitiscells in suspension culture, Agricultural and Biological Chemistry, 47, 2185, doi.org/10.1271/bbb1961.47.2185 ; SakamotoK (1993), Effects of nutrients on anthocyanin production in cultured cells ofAralia cordata, Phytochemistry, 33, 357, doi.org/10.1016/0031-9422(93)85517-U ; LightbournGJ (2007), Epistatic interaction influencing anthocyanin gene expression inCapsicum annuum, Journal of the American Society for Horticultural Science, 132, 824. ; TadaH (1996), Anthocyanin inLobelia chinensishairy roots Plant Tissue Culture, Letters, 13, 85.