Tytuł artykułu

The Alleviation of the Adverse Effects of Salt Stress in the Tomato Plant by Salicylic Acid Shows A Time- and Organ-Specific Antioxidant Response

Tytuł czasopisma

Acta Biologica Cracoviensia s. Botanica




No 1

Autorzy publikacji

Wydział PAN

Nauki Biologiczne i Rolnicze


<jats:title>Abstract</jats:title> <jats:p>In the last decade contradictory results have been published as to whether exogenous salicylic acid (SA) can increase salt stress tolerance in cultivated plants by inducing an antioxidant response. Salt stress injury in tomato was mitigated only in cases when the plant was hardened with a high concentration of SA (~10<jats:sup>−4</jats:sup> M), low concentrations were ineffective. An efficient accumulation of Na<jats:sup>+</jats:sup> in older leaves is a well-known response to salt stress in tomato plants (<jats:italic>Solanum lycopersicum</jats:italic> cv. Rio fuego) but it remains largely unexplored whether young and old leaves or root tissues have a distinct antioxidant status during salt stress after hardening with 10<jats:sup>−7</jats:sup> M or 10<jats:sup>−4</jats:sup> M SA. The determination of superoxide dismutase (SOD) and catalase (CAT) activity revealed that the SA-induced transient increases in these enzyme activities in young leaf and/or root tissues did not correlate with the salt tolerance of plants. Salt stress resulted in a tenfold increase in ascorbate peroxidase (APX) activities of young leaves and significant increases in APX and glutathione reductase (GR) activities of the roots hardened with 10<jats:sup>−4</jats:sup> M SA. Both total ascorbate (AsA) and glutathione pools reached their highest levels in leaves after 10<jats:sup>−7</jats:sup> M SA pre-treatment. However, in contrast to the leaves, the total pool of AsA decreased in the roots under salt stress and thus, due to low APX activity, active oxygen species were scavenged by ascorbate non-enzymatically in these tissues. The increased GR activities in the roots after treatment with 10<jats:sup>−4</jats:sup> M SA enabled plants to enhance the reduced glutathione (GSH) pool and maintain the redox status of AsA under high salinity, which led to increased salt tolerance.</jats:p>


Biological Commission of the Polish Academy of Sciences – Cracow Branch


2015[2015.01.01 AD - 2015.12.31 AD]


eISSN 1898-0295 ; ISSN 0001-5296


AnanievaEA (2004), Exogenous treatment with salicylic acid leads to increased antioxidant capacity in leaves of barley plants exposed to paraquat, Journal of Plant Physiology, 161. ; SzepesiÁ (2009), Salicylic acid improves acclimation to salt stress by stimulating abscisic aldehyde oxidase activity and abscisic acid accumulation , and increases Na + content in leaves without toxicity symptoms inSolanum lycopersicumL, Journal of Plant Physiology, 166. ; MunnsR (2008), Mechanisms of salinity tolerance, Annual Review of Plant Biology, 59. ; PoórP (2011), Salicylic acid treatment via the rooting medium interferes with stomatal response , CO fixation rate and carbohydrate metabolism in tomato , and decreases harmful effects of subsequent salt stress, Plant Biology, 13. ; LawMY (1983), Glutathione and ascorbic acid in spinach ( Spinacia oleracea ) chloroplasts The effect of hydrogen peroxide and of paraquat, Biochemical Journal, 210. ; WangLJ (2006), Salicylic acid - induced heat or cold tolerance in relation to Ca homeostasis and antioxidant systems in young grape plants, Plant Science, 170. ; SunW (2010), Comparative transcriptomic profiling of a salt - tolerant wild tomato species and a salt - sensitive tomato cultivar, Plant Cell Physiology, 51. ; FoyerCH (2011), Ascorbate and glutathione : the heart of the redox hub, Plant Physiology, 155. ; ShiQ (2006), Effects of different treatments of salicylic acid on heat tolerance , chlorophyll fluorescence , and antioxidant enzyme activity in seedlings ofCucumis sativusL, Plant Growth Regulation, 48. ; CsiszárJ (2004), Auxin autotrophic tobacco callus tissues resist oxidative stress : the importance of glutathione S - transferase and glutathione peroxidase activities in auxin heterotrophic and autotrophic calli, Journal of Plant Physiology, 161. ; AebiH (1984), Catalasein vitro, Methods in Enzymology, 105. ; NakanoY (1987), Purification of ascorbate peroxidase in spinach chloroplasts ; its inactivation in ascorbate - depleted medium and reactivation by monodehydroascorbate radical, Plant Cell Physiology, 28. ; JuanM (2005), Evaluation of some nutritional and biochemical indicators in selecting salt - resistant tomato cultivars, Environmental and Experimental Botany, 54. ; DurnerJ (1995), Inhibition of ascorbate peroxidase by salicylic acid and dichloroisonicotinic acid , two inducers of plant defense responses Proceedings of National Academy of, Sciences, 11312. ; AsadaK (1999), The water - water cycle in chloroplasts : scavenging of active oxygens and dissipation of excess photons, Annual Review of Plant Biology, 50. ; BocováB (2012), Cadmium disrupts apoplastic ascorbate redox status in barley root tips, Acta Physiologiae Plantarum, 34. ; LiG (2013), Salicylic acid increases the contents of glutathione and ascorbate and temporally regulates the related gene expression in salt - stressed wheat seedlings, Gene, 529. ; GémesK (2011), Cross - talk between salicylic acid and NaCl - generated reactive oxygen species and nitric oxide in tomato during acclimation to high salinity, Physiologia Plantarum, 142. ; TariI (2002), a Acclimation of tomato plants to salinity stress after a salicylic acid pretreatment, Acta Biologica Szegediensis, 46. ; JandaT (2003), Comparative study of frost tolerance and antioxidant activity in cereals, Plant Science, 164. ; HaoJH (2012), Insights into salicylic acid responses in cucumber ( Cucumis sativusL ) cotyledons based on a comparative proteomic analysis, Plant Science, 18. ; ShalataA (2001), Response of cultivated tomato and its wild salt - tolerant relativeLycopersicon penneliito salt - dependent oxidative stress The root antioxidative system, Physiologia Plantarum, 112. ; TariI (2002), b Changes in thiol content in roots of wheat cultivars exposed to copper stress, Biologia Plantarum, 45. ; FooladMR (2001), Identification and validation of QTLs for salt tolerance during vegetative growth in tomato by selective genotyping, Genome, 44. ; CsiszárJ (2014), Glutathione transferase supergene family in tomato : Salt stress - regulated expression of representative genes from distinct GST classes in plants primed with salicylic acid, Plant Physiology and Biochemistry, 78. ; AlscherRG (2002), Role of superoxide dismutases ( SODs ) in controlling oxidative stress in plants, Journal of Experimental Botany, 53. ; GriffithOW (1980), Determination of glutathione and glutathione disulfide using glutathione reductase and - vinylpyridine, Analytical Biochemistry, 106. ; KocsyG (2013), Redox control of plant growth and development, Plant Science, 211. ; HorváthE (2002), In vitrosalicylic acid inhibition of catalase activity in maize : differences between the isozymes and a possible role in the induction of chilling tolerance, Plant Science, 163. ; ArfanM (2007), Does exogenous application of salicylic acid through the rooting medium modulate growth and photosynthetic capacity in two differently adapted spring wheat cultivars under salt stress, Journal of Plant Physiology, 164. ; CuarteroJ (1992), Selection of donors for salt tolerance in tomato using physiological traits, New Phytologist, 121. ; DatJF (1998), Parallel changes in and catalase during thermotolerance induced by salicylic acid or heat acclimation in mustard seedlings, Plant Physiology, 116. ; SzepesiA (2005), Role of salicylic acid pre - treatment on the acclimation of tomato plants to salt - and osmotic stress, Acta Biologica Szegediensis, 49. ; Córdoba (2003), Zonal changes in ascorbate and hydrogen peroxide contents , peroxidase and ascorbate - related enzyme activities in onion roots, Plant Physiology, 131. ; BeauchamPC (1971), Superoxide dismutase : improved assays and an assay applicable to acrylamide gels, Analytical Biochemistry, 44. ; BorsaniO (2001), Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress inArabidopsisseedlings, Plant Physiology, 126. ; CuarteroJ (2002), Variability for some physiological characters affecting salt tolerance in tomato, Acta Horticulturae, 573. ; AshrafMPJC (2004), Potential biochemical indicators of salinity tolerance in plants, Plant Science, 166. ; HorváthE (2007), Induction of abiotic stress tolerance by salicylic acid signaling, Journal of Plant Growth Regulation, 26. ; TariI (2010), Salicylic acid increased aldose reductase activity and sorbitol accumulation in tomato plants under salt stress, Biologia Plantarum, 54. ; ManaaA (2011), Salt and genotype impact on plant physiology and root proteome variations in tomato, Journal of Experimental Botany, 62.