Miscanthus: Inter- and Intraspecific Genome Size Variation Among M. × Giganteus, M. Sinensis, M. Sacchariflorus Accessions

Journal title

Acta Biologica Cracoviensia s. Botanica




No 1

Publication authors

Divisions of PAS

Nauki Biologiczne i Rolnicze


<jats:title>Abstract</jats:title> <jats:p>Since <jats:italic>M. sinensis</jats:italic> Anderss., <jats:italic>M. sacchariflorus</jats:italic> (Maxim.) Hack. and <jats:italic>M. ×giganteus</jats:italic> J.M.Greef &amp; Deuter ex Hodk. and Renvoize have considerably the highest potential for biomass production among <jats:italic>Miscanthus</jats:italic> Anderss. species, there is an urgent need to broaden the knowledge about cytological characteristics required for their improvement. In this study our objectives were to assess the genome size variation among eighteen <jats:italic>Miscanthus</jats:italic> accessions, as well as estimation of the monoploid genome size (2C and Cx) of the <jats:italic>M. sinensis</jats:italic> cultivars, which have not been analyzed yet. The characterization of three <jats:italic>Miscanthus</jats:italic> species was performed with the use of flow cytometry and analysis of the stomatal length. The triploid (2n = 3x = 57) <jats:italic>M. sinensis</jats:italic> ‘Goliath’ and <jats:italic>M. ×giganteus</jats:italic> clones possessed the highest 2C DNA content (8.34 pg and 7.43 pg, respectively). The intermediate 2C-values were found in the nuclei of the diploid (2n = 2x = 38) <jats:italic>M. sinensis</jats:italic> accessions (5.52–5.72 pg), whereas they were the lowest in the diploid (2n = 2x = 38) <jats:italic>M. sacchariflorus</jats:italic> ecotypes (4.58–4.59 pg). The presented study revealed interspecific variation of nuclear DNA content (P&lt;0.01) and therefore allowed for recognition of particular taxa, inter- and intraspecific hybrids and prediction of potential parental components. Moreover, intraspecific genome size variation (P&lt;0.01) was observed in <jats:italic>M. sinensis</jats:italic> cultivars at 3.62%. The values of the stomatal size obtained for the triploid <jats:italic>M. ×giganteus</jats:italic> ‘Great Britain’ (mean 30.70 μm) or ‘Canada’ (mean 29.67 μm) and diploid <jats:italic>M. sinensis</jats:italic> ‘Graziella’ (mean 29.96 μm) did not differ significantly, therefore this parameter is not recommended for ploidy estimation.</jats:p>


Biological Commission of the Polish Academy of Sciences – Cracow Branch


2015[2015.01.01 AD - 2015.12.31 AD]


eISSN 1898-0295 ; ISSN 0001-5296


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