Szczegóły

Tytuł artykułu

Adjustments to In Vitro Culture Conditions and Associated Anomalies in Plants

Tytuł czasopisma

Acta Biologica Cracoviensia s. Botanica

Rocznik

2015

Numer

No 2

Autorzy publikacji

Wydział PAN

Nauki Biologiczne i Rolnicze

Abstrakt

Abstract Plant tissue culture techniques have become an integral part of progress in plant science research due to the opportunity offered for close study of detailed plant development with applications in food production through crop improvement, secondary metabolites production and conservation of species. Because the techniques involve growing plants under controlled conditions different from their natural outdoor environment, the plants need adjustments in physiology, anatomy and metabolism for successful in vitro propagation. Therefore, the protocol has to be optimized for a given species or genotype due to the variability in physiological and growth requirement. Developing the protocol is hampered by several physiological and developmental aberrations in the anatomy and physiology of the plantlets, attributed to in vitro culture conditions of high humidity, low light levels and hetero- or mixotrophic conditions. Some of the culture-induced anomalies become genetic, and the phenotype is inherited by clonal progenies while others are temporary and can be corrected at a later stage of protocol development through changes in anatomy, physiology and metabolism. The success of protocols relies on the transfer of plantlets to field conditions which has been achieved with many species through stages of acclimatization, while with others it remains a challenging task. This review discusses various adjustments in nutrition, physiology and anatomy of micro-propagated plants and field grown ones, as well as anomalies induced by the in vitro culture conditions.

Wydawca

Biological Commission of the Polish Academy of Sciences – Cracow Branch

Data

2015[2015.01.01 AD - 2015.12.31 AD]

Identyfikator

eISSN 1898-0295 ; ISSN 0001-5296

Referencje

CassellsAC (1997), Evaluation of image analysis flow cytometry and RAPD analysis for the assessment of somaclonal variation and induced mutation in tissue culture - derivedPelargoniumplants, Angewandte Botanik, 71, 119. ; MoyoM (2014), Photoperiod and plant growth regulator combinations influence growth and physiological responses inPelargonium sidoides In vitro Cellular and Developmental, Biology Plant, 50, 487. ; vanStadenJ (2006), Plant stressin vitro : The role of phytohormones, Acta Horticulturae, 725, 55, doi.org/10.17660/ActaHortic.2006.725.2 ; MonteuuisO (2008), DNA methylation in different origin clonal offspring from matureSequoiadendron giganteumgenotype Trees Structure, and Function, 22, 779. ; PapafotiouM (2001), In vitroplant regeneration of theMammillaria elongatanormal and cristate forms, Plant Cell Tissue and Organ Culture, 65, 163, doi.org/10.1023/A:1010601328667 ; PerryPL (1999), Reversion to hyperhydration by the addition of antibiotics to removePseudomonasin unhyperhydrated oregano tissue culture, Process Biochemistry, 34, 717, doi.org/10.1016/S0032-9592(98)00146-0 ; LongS (1994), Photoinhibition of photosynthesis in nature, Annual Review of Plant Physiology and Plant Molecular Biology, 45, 633, doi.org/10.1146/annurev.pp.45.060194.003221 ; GriffisJL (1983), Establishing tissue cultured plants in soil Combined Proceeding International Plant Propagators Society, Journal, 33, 618. ; SaezP (2012), a Photosynthetic and leaf anatomical characteristics ofCastanea sativa : a comparison betweenin vitroand nursery plants, Biologia Plantarum, 56, 15, doi.org/10.1007/s10535-012-0010-9 ; RodriguesSP (2014), The effects ofin vitroculture on the leaf anatomy ofJatropha curcas, Bioscience Journal, 30, 1933. ; KaragiozovaM (1977), Histological characteristic of band - shaped fasciations, Annual Journal of Sofia University, 68, 11. ; Us (2014), and De - la - PenaC In vitroculture : an epigenetic challenge for plants, Plant Cell Tissue and Organ Culture, 118, 187, doi.org/10.1007/s11240-014-0482-8 ; WangQM (2012), Direct and indirect organogenesis ofClivia miniataand assessment of DNA methylation changes in various regenerated plantlets, Plant Cell Reports, 31, 1283, doi.org/10.1007/s00299-012-1248-6 ; MendozaM (2002), Auxin and sugar effects on callus induction and plant regeneration frequencies from mature embryos of wheat ( Triticum aestivumL ) In vitro Cellular and Developmental, Biology Plant, 38, 39, doi.org/10.1079/IVP2001250 ; IsraeliY (1996), Selection of stable banana clones which do not produce dwarf somaclonal variants duringin vitroculture, Scientia Horticulturae, 67, 197, doi.org/10.1016/S0304-4238(96)00955-7 ; CrespiM (1992), Fasciation induction by the phytopathogenRhodococcus fasciansdepends upon linear plasmid encoding a cytokinin synthase gene, European Molecular Biology Organization Journal, 11, 795. ; DaleJE (1988), The control of leaf expansion, Annual Review of Plant Physiology and Molecular Biology, 39, 267, doi.org/10.1146/annurev.pp.39.060188.001411 ; PenceVC (2010), The possibilities and challenges ofin vitromethods for plant conservation, Kew Bulletin, 65, 539, doi.org/10.1007/s12225-011-9245-4 ; JarilloJA (2009), Chromatin remodeling in plant development InternationalJournal of, Developmental Biology, 53, 1581. ; WojtaniaA (2015), Sucrose and cytokinin interactions in relation to ethylene and abscisic acid production in the regulation of morphogenesis inPelargonium hortorumLH Baileyin vitro, Acta Biologica Cracoviensia Series Botanica, 57, 62. ; TothS (2004), Effective and reproducible protocol forin vitroculturing and plant regeneration of the physiological model plantRamonda myconi L ) Rchb, Plant Science, 166, 1027, doi.org/10.1016/j.plantsci.2003.12.020 ; KeversC (1986), Vitrification of carnationin vitro : changes in content extracellular space air volume and ion levels, Physiologie Vegetale, 24, 647. ; RohMS (1989), In vitroproduction ofAchimenantha Inferno as influenced by season and the source of the explants, Acta Horticlturae, 252, 129. ; KowalskiB (1999), Mutation breeding for yield and Phytophthora infestans Mont foliar resistance in potato ( Solanum tuberosumL cv Golden Wonder ) using computerized image analysis in selection, Potato Research, 42, 121, doi.org/10.1007/BF02358402 ; BarghchiM (1996), The control of shoot - tip necrosis inPistacia veraL in vitro, Plant Growth Regulation, 20, 31, doi.org/10.1007/BF00024054 ; HagegeD (1995), Habituation in plant cell cultures : adaptation to the free radical attacks, Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales, 189, 1183. ; RodriguesPHV (2008), Somaclonal variation in micropropagatedHeliconia bihaicv Lobster Claw I plantlets ( Heliconiaceae ), Scientia Agricola, 65, 681. ; RadhakrishnanR (2008), Morphological and agronomic evaluation of tissue culture - derived Indian soybean plants, Acta Agriculturae Slovenica, 91, 391. ; DeanRiderS (2003), Coordinate repression of regulators of embryonic identity by a PICKLE during germination in theArabidopsis, Plant Journal, 35, 33, doi.org/10.1046/j.1365-313X.2003.01783.x ; MiguelC (2011), An epigenetic view of plant cells cultured in thein vitro : somaclonal variation and beyond, Journal of Experimental Botany, 62, 3713, doi.org/10.1093/jxb/err155 ; ApterRC (1993), a In vitroandex vitroadventitious root formation in Asian jasmine ( Trachelospermum asiaticum ) II : physiological comparisons for, Journal of American Society Hortucultural Science, 118, 906. ; Akin (2009), Tissue culture as plant production technique for horticultural crops, African Journal of Biotechnology, 8, 3782. ; HazarikaBN (2001), Biochemical basis of acclimatization of micropropagated plantlets : A review, Agricultural Review, 22, 65. ; WhiteOE (1945), The biology of fasciation, Journal of Heredity, 36, 11, doi.org/10.1093/oxfordjournals.jhered.a105409 ; SivanesanI (2007), Shoot regeneration and somaclonal variation from leaf callus cultures ofPlumbago zeylanicaLinn, Asian Journal of Plant Science, 6, 83, doi.org/10.3923/ajps.2007.83.86 ; ChristiansonML (1985), Temporal requirements for the phytohormone balance in the control of organogenesisin vitro, Developmental Biology, 112, 494, doi.org/10.1016/0012-1606(85)90423-3 ; GasparT (1998), A typical metabolisms and biochemical cycles imposing the cancerous state on plant cells, Plant Growth Regulation, 24, 135, doi.org/10.1023/A:1005972924568 ; DonnellyDJ (1984), Pigment content and gas exchange of red raspberryin vitroandex vitro for, Journal of American Society Horticultural Science, 109, 177. ; LavanyaM (2009), Acclimatization of neem micro shoots adaptable to semisterile conditions, Indian Journal of Biotechnology, 8, 218. ; HazarikaBN (2003), Acclimatization of tissue - cultured plants, Current Science, 85, 1704. ; AlmeidaR (2005), RNA silencing and genome regulation, Trends in Cell Biology, 15, 251, doi.org/10.1016/j.tcb.2005.03.006 ; DonnellyDJ (1985), The anatomy of tissue - cultured red raspberry prior to and after transfer to soil, Plant Cell Tissue and Organ Culture, 4, 43, doi.org/10.1007/BF00041654 ; DeberghPC (1981), Mass propagation of globe actichoke ( Synara scolymus ) : evaluation of different hypothesis to overcome vitrification with special reference to water potential, Physiologia Plantarum, 53, 181, doi.org/10.1111/j.1399-3054.1981.tb04130.x ; HazarikaBN (2002), Anatomical variation in Citrus leaves fromin vitroand greenhouse plants : scanning electron microscopic studies, Indian Journal of Horticulture, 59, 243. ; GrafiG (2007), Histone methylation controls telomerase - independent telomere lengthening in cells undergoing dedifferentiation, Developmental Biology, 306, 838, doi.org/10.1016/j.ydbio.2007.03.023 ; PospisilovaJ (1998), Acclimation of tobacco plantlets toex vitrocondition as affected by the application of ABA, Journal of Experimental Botany, 49, 863, doi.org/10.1093/jxb/49.322.863 ; ThomasP (2000), Explant medium and vessel aeration affect the incidence of hyperhydricity and recovery of normal plantlets in triploid watermelon, Journal of Horticultural Science and Biotechnology, 75, 19, doi.org/10.1080/14620316.2000.11511194 ; BairuMW (2008), The role of topolins in micropropagation and somaclonal variation of banana cultivars Williams and Grand Naine ( Musaspp AAA ), Plant Cell Tissue and Organ Culture, 95, 373, doi.org/10.1007/s11240-008-9451-4 ; LarkinPJ (1981), Somaclonal variation a novel source of variability from cell cultures for the plant improvement, Theoretical and Applied Genetics, 60, 197, doi.org/10.1007/BF02342540 ; FambriniM (2006), Stem fasciated : a recessive mutation in the sunflower ( Helianthus annuus ) alters plant morphology and auxin level, Annals of Botany, 98, 715, doi.org/10.1093/aob/mcl153 ; DonesoI (1987), Factors influencing vitrification of carnation and conifers, Acta Horticulturae, 212, 67. ; HsuHC (2010), The effect of light spectrum on growth characteristics ofin vitrocultures ofPhalaenopsis, Propagation of Ornamental Plants, 10, 3. ; TichaI (1998), Cultures on sugar medium enhance photosynthetic capacity and high light resistance of plantlets grownin vitro, Physiologia Plantarum, 102, 155, doi.org/10.1034/j.1399-3054.1998.1020201.x ; BottcherI (1988), Inducting and reversion of vitrification of plants culturedin vitro, Physiologia Plantarum, 66, 94. ; GillyC (1997), Ultrastructure and radiolabeling of leaf cuticles from the ivy ( Hedera helixL ) plantsin vitroand duringex vitroacclimatization, Annals of Botany, 80, 139, doi.org/10.1006/anbo.1997.0414 ; SchnablePS (2009), The B maize genome : complexity diversity and dynamics, Science, 326, 1112, doi.org/10.1126/science.1178534 ; AnzolaJM (2010), PutativeArabidopsistranscriptional adaptor protein ( PROPORZ ) is required to modulate histone acetylation in response to auxin Proceeding of the National Academy of, Science USA, 1, 10308, doi.org/10.1073/pnas.0913918107 ; DesjardinsY (1988), Effect of CO enrichment and high photosynthetic photon flux on the development of autotrophy and growth of cultured tissue strawberry raspberry and asparagus plants, Acta Horticulturae, 230, 47. ; ViejoM (2010), DNA methylation during sexual embryogenesis and implications on the induction of somatic embryogenesis in Castanea sativa Miller, Sexual Plant Reproduction, 23, 315, doi.org/10.1007/s00497-010-0145-9 ; Perez (2001), Control of hyperhydricity in micropropagated apricot cultivars In vitro Cellular and Developmental, Biology Plant, 37, 250. ; CassellsA (2001), Oxidative stress and physiological epigenetic and genetic variability in plant tissue culture : implications for micro - propagators and genetic engineers, Plant Cell Tissue and Organ Culture, 64, 145, doi.org/10.1023/A:1010692104861 ; PeschkeVM (1992), Genetic implications of somaclonal variation in plants, Advances in Genetics, 30, 41, doi.org/10.1016/S0065-2660(08)60318-1 ; MitrasD (2009), In vitropropagation ofFraxinus excelsiorby epicotyls, Journal of Biological Research Thessaloniki, 11, 37. ; JoyceSM (2002), Variation in potato micro plant morphologyin vitroand DNA methylation, Plant Cell Tissue and Organ Culture, 70, 125, doi.org/10.1023/A:1016312303320 ; SahayNS (2000), A biological approach towards increasing the rates of survival of micropropagated plants, Current Science, 78, 126. ; MoyoM (2015), Insights into the multifaceted application of microscopic techniques in plant tissue culture systems, Planta, 242, 773, doi.org/10.1007/s00425-015-2359-4 ; BrautigamK (2013), Epigenetic regulation of adaptive responses of forest tree species to the environment, Ecology and Evolution, 3, 399, doi.org/10.1002/ece3.461 ; ZivM (1987), Malfunctioning stomata in vitreous leaves of carnation plants propagatedin vitro : implication for hardening, Plant Science, 52, 127, doi.org/10.1016/0168-9452(87)90114-2 ; VitkovskiiV (1959), Fasciation ofSyringa josikaeashoots, Botanicheskii Journal, 44, 505. ; ShettyS (1996), Reduction of hyperhydricity in tissue cultures of oregano ( Origanum vulgare ) by extracellular polysaccharide isolated fromPseudomonasspp, Plant Science, 120, 175, doi.org/10.1016/S0168-9452(96)04482-2 ; GroutBWW (1985), Photosynthetic development of the micropropagated strawberry plantlets following transplanting, Annals of Botany, 55, 129. ; IkeuchiM (2013), Plant callus : mechanisms of induction and repression, Plant Cell, 25, 3159, doi.org/10.1105/tpc.113.116053 ; KaepplerSM (2000), Epigenetic aspects of somaclonal variation in plants, Plant Molecular Biology, 43, 179, doi.org/10.1023/A:1006423110134 ; SharmaS (2007), Stability of potato ( Solanum tuberosumL ) plants regenerated via somatic embryos axillary bud proliferated shoots micro tubers and true potato seeds : a comparative phenotypic cytogenetic and molecular assessment, Planta, 226, 1449, doi.org/10.1007/s00425-007-0583-2 ; RossettoM (1992), Aeration : A simple method to control vitrification and improvein vitroculture of rare Australian plants In vitro Cellular and Developmental, Biology Plant, 28, 192. ; FuchigamiLH (1981), Abaxial transpiration and water loss in aseptically cultured plum for, Journal of American Society Horticultural Science, 106, 519. ; JaligotE (2000), Somaclonal variation in oil palm ( Elaeis guineensisJacq ) : the DNA methylation hypothesis, Plant Cell Reports, 19, 684, doi.org/10.1007/s002999900177 ; ZivM (1998), Proliferation of meristematic clusters in disposable pre - sterilized plastic bioreactors for large - scale micropropagation of plants In vitro Cellular and Developmental, Biology Plant, 34, 152. ; KataevaNV (1991), Effects of applied and internal hormones on vitrification and apical necrosis of different plants culturedin vitro, Plant Cell Tissue and Organ Culture, 14, 31. ; Diaz (1995), a Effect ofin vitro - formed roots and acclimatization on water status and gas exchange of tissue - cultured apple shoots for, Journal of American Society Horticultural Science, 120, 435. ; GasparT (1996), Plant hormones and plant growth regulators in plant tissue culture In vitro, Cellular Developmental Biology Plant, 32, 272, doi.org/10.1007/bf02822700 ; TangY (1998), Fasciation mutation enhances meristematic activity and alters pattern formation in soybean, International Journal of Plant Science, 159, 249, doi.org/10.1086/297546 ; GreenwoodMS (1987), Rejuvenation of forest trees, Plant Growth Regulation, 6, 1, doi.org/10.1007/BF00043947 ; FuY (2013), Mobilization of a plant transposon by expression of the transposon - encoded antisilencing factor, European Molecular Biology Organization Journal, 32, 2407, doi.org/10.1038/emboj.2013.169 ; BhattID (2000), Combined effects of cytokinins on the multiple shoot production from cotyledonary node explants ofBauhinia vahlii, Plant Cell Tissue and Organ Culture, 62, 79, doi.org/10.1023/A:1006450516329 ; ZeigerE (1983), The biology of stomatal guard cells, Annual Review of Plant Physiology, 34, 441, doi.org/10.1146/annurev.pp.34.060183.002301 ; SmithZD (2013), DNA methylation : roles in mammalian development, Nature Reviews Genetics, 14, 204, doi.org/10.1038/nrg3354 ; ChristouP (1988), Habituation inin vitrosoybean cultures, Plant Physiology, 88, 809, doi.org/10.1104/pp.87.4.809 ; BirdA (2007), Perceptions of epigenetics, Nature, 447. ; ClarkSE (1993), CLAVATA a regulator of meristem and flower development inArabidopsis, Development, 119, 397. ; VieitezAM (1985), Anatomical and chemical studies of vitrified shoots of chestnut regeneratedin vitro, Physiologia Plantarum, 65, 177, doi.org/10.1111/j.1399-3054.1985.tb02379.x ; BaurensFC (2004), Genomic DNA methylation of juvenile and matureAcacia mangiummicropropagatedin vitrowith reference to leaf morphology as a phase change marker, Tree Physiology, 24, 401, doi.org/10.1093/treephys/24.4.401 ; Kei (1998), Reduced hyperhydricity and enhanced growth of tissue culture - generated raspberry ( Rubussp ) clonal lines byPseudomonassp isolated from oregano, Process Biochemistry, 33, 441, doi.org/10.1016/S0032-9592(98)00011-9 ; BertacciniA (2005), Molecular characterization of phytoplasmas in lilies with fasciation in the Czech Republic, FEMS Microbiology Letters, 249, 79, doi.org/10.1016/j.femsle.2005.06.001 ; VogelG (2005), Deriving controversy - free ES cells is controversial, Science, 310, 416, doi.org/10.1126/science.310.5747.416a ; SudhaCG (2000), Regeneration of plants from thein vitroroot segments ofHolostemma annulare a rare medicinal plant, Current Science, 78, 503. ; SmuldersM (2011), Epigenetics in plant tissue culture, Plant Growth Regulation, 63, 137, doi.org/10.1007/s10725-010-9531-4 ; WetzsteinHY (1982), Leaf anatomy of tissueculturedLiquidambar styracifluaduring acclimatization, American Journal of Botany, 69, 1579, doi.org/10.2307/2442913 ; JaritehM (2015), Developmental changes in protein proline and some antioxidant enzymes activities in somatic and zygotic embryos of Persian walnut ( Juglans regiaL ), Plant Cell Tissue and Organ Culture, 122, 101, doi.org/10.1007/s11240-015-0753-z ; FragaM (2002), Phase - change related epigenetic and physiological changes inPinus radiataD Don, Planta, 215, 672, doi.org/10.1007/s00425-002-0795-4 ; Rodriguez (2011), MicroRNA misregulation : an overlooked factor generating somaclonal variation, Trends in Plant Science, 16, 242, doi.org/10.1016/j.tplants.2011.03.002 ; PhanCT (1983), A comparative study of chlorophyll phenolic and protein contents and hydroxycinnamate : CoA ligase activity of normal and vitreous plants ( Prunus aviumL ) obtainedin vitro, Plant Science Letters, 31, 323, doi.org/10.1016/0304-4211(83)90071-8 ; MeinsF (1985), Reversible cell - heritable changes during the development of tobacco pith tissues, Developmental Biology, 108, 1, doi.org/10.1016/0012-1606(85)90002-8 ; SahijramL (2003), Analyzing somaclonal variation in micropropagated bananas ( Musaspp ) In vitro Cellular and Developmental, Biology Plant, 39, 551. ; ZielinskiO (1945), Fasciation in the horticultural plants with special reference to tomato Proceeding of for, American Society Horticultural Science, 46, 263. ; SarkerD (2002), Growing of potato micro plants in the presence of alginate - silver thiosulfate capsules reduces ethylene - induced cultures abnormalities during minimal growth conservationin vitro, Plant Cell Tissue and Organ Culture, 68, 79, doi.org/10.1023/A:1012904917745 ; Diaz (1995), b Acclimatization and subsequent gas exchange water relations survival and growth of micro cultured apple plantlets after transplanting them in soil, Physiologia Plantarum, 95, 225, doi.org/10.1034/j.1399-3054.1995.950208.x ; SharmaU (2006), Reduction of vitrification inin vitroraised shoots ofChlorophytum borivilianumSant & Fernand : a rare potent medicinal herb, Indian Journal of Experimental Biology, 44, 499. ; ReuveniO (1990), Measures to reduce somaclonal variation inin vitro - propagated bananas, Acta Horticulturae, 275, 307, doi.org/10.17660/ActaHortic.1990.275.37 ; MayorML (2003), Reduction of hyperhydricity in sunflower tissue culture, Plant Cell Tissue and Organ Culture, 72, 99, doi.org/10.1023/A:1021216324757 ; De (2012), KNOX is expressed and epigenetically regulated duringin vitroconditions inAgavespp, BMC Plant Biology, 12, 203, doi.org/10.1186/1471-2229-12-203 ; CausevicA (2006), Relationship between DNA methylation and histone acetylation levels cell redox and cell differentiation states in sugarbeet lines, Planta, 224, 812, doi.org/10.1007/s00425-006-0267-3 ; ValledorL (2007), Involvement of DNA methylation in tree development and micropropagation, Plant Cell Tissue and Organ Culture, 91, 75, doi.org/10.1007/s11240-007-9262-z ; RoutGR (2000), In vitromanipulation and propagation of medicinal plants, Biotechnology Advances, 18, 91, doi.org/10.1016/S0734-9750(99)00026-9 ; KhanS (2003), Growth and water relations ofPaulownia fortuneiunder photomixotrophic and photoautrotrophic conditions, Biologia Plantarum, 46, 161, doi.org/10.1023/A:1022844720795 ; LevaAR (2012), Somaclonal variation in tissue culture a case study with olive http : / / dx doi org, Intech Open Science, 123, doi.org/10.5772/50367 ; ChavezMM (1991), Effects of water deficits on carbon assimilation, Journal of Experimental Botany, 42, 1, doi.org/10.1093/jxb/42.1.1 ; HazarikaBN (2006), Morpho - physiological disorders inin vitrocultured plants, Scientia Horticulturae, 108, 105, doi.org/10.1016/j.scienta.2006.01.038 ; RoelsS (2005), Optimisation of plantain ( MusaAAB ) micropropagation by temporary immersion systems, Plant Cell Tissue and Organ Culture, 82, 57, doi.org/10.1007/s11240-004-6746-y ; NilssonO (1996), Expression of theAgrobacterium rhizogenesrolC gene in a deciduous forest tree alters growth and development and leads to stem fasciation, Plant Physiology, 112, 493. ; AlbertsenMC (1983), Genetics and comparative growth morphology of fasciation in soybeans ( Glycine max L ] Merr ), Botanical Gazette, 144, 263, doi.org/10.1086/337372 ; SrivastavaA (2013), Control of shoot tip necrosis in shoot cultures ofPortulaca grandifloraHook, Notulae Scientia Biologicae, 5, 45. ; GalzyR (1992), Remarks on mixotrophic and autotrophic carbon nutrition ofVitisplantlets culturedin vitro, Plant Cell Tissue and Organ Culture, 31, 239. ; SmykalP (2007), Assessment of genetic and epigenetic stability in longtermin vitroshoot culture of pea ( Pisum sativumL ), Plant Cell Reports, 26, 1985, doi.org/10.1007/s00299-007-0413-9 ; MukherjeeSK (1991), Low sugar and osmotic requirement of shoot regeneration from leaf pieces ofSolanum melogenaL, Plant Cell Tissue and Organ Culture, 25, 13, doi.org/10.1007/BF00033906 ; CarvalhoL (2001), Chlorophyll fluorescence as indicator of photosynthetic functioning ofin vitrograpevine and chestnut plantlets underex vitroacclimatization, Plant Cell Tissue and Organ Culture, 67, 271, doi.org/10.1023/A:1012722112406 ; PospisilovaJ (1999), Acclimatization of micropropagated plants inex vitroconditions, Biologia Plantarum, 42, 481, doi.org/10.1023/A:1002688208758 ; LamhamediMS (2003), Epidermal transpiration ultrastructural characteristics and net photosynthesis of white spruce somatic seedlings in response toin vitroacclimatization, Physiologia Plantarum, 118, 554, doi.org/10.1034/j.1399-3054.2003.00146.x ; BairuMW (2009), Factors contributing toin vitroshoot tip necrosis and their physiological interactions, Plant Cell Tissue and Organ Culture, 98, 239, doi.org/10.1007/s11240-009-9560-8 ; RaniV (2000), Genetic fidelity of organized meristem - derived micro - propagated plants : a critical reappraisal In vitro Cellular and Developmental, Biology Plant, 36, 319. ; PhillipsRL (1994), Genetic instability of plant tissue cultures : a break - down of normal controls Proceeding of National Academy of, Science USA, 91, 5222, doi.org/10.1073/pnas.91.12.5222 ; JoyceSM (1999), PCR analysis of bisulphite - modified DNA to investigate developmental variation in micro - propagated potatoes ( Solanum tuberosumL ), Potato Research, 42, 549, doi.org/10.1007/BF02358171 ; SutterE (1984), Chemical composition of the epicuticular wax in the cabbage plants grownin vitro, Canadian Journal of Botany, 62, 74, doi.org/10.1139/b84-012 ; ChenM (2010), Epigenetic performers in plants, Development Growth and Differentiation, 52, 555, doi.org/10.1111/j.1440-169X.2010.01192.x ; SiragusaM (2007), Genetic instability in calamondin ( Citrus madurensisLour ) plants derived from somatic embryogenesis induced by diphenylurea - derivatives, Plant Cell Reports, 26, 1289, doi.org/10.1007/s00299-007-0326-7 ; SaezPL (2012), b Increased light intensity duringin vitroculture improves water loss control and photosynthetic performance ofCastanea sativagrown in ventilated vessels, Scientia Horticulturae, 138, 7, doi.org/10.1016/j.scienta.2012.02.005 ; MeinsF (1989), Habituation : heritable variation in the requirement of cultured plant cells for hormones, Annual Review of Genetics, 23, 395, doi.org/10.1146/annurev.ge.23.120189.002143 ; JinS (2008), Detection of somaclonal variation of cotton ( Gossypium hirsutum ) using cytogenetics flow cytometry and molecular markers, Plant Cell Reports, 27, 1303, doi.org/10.1007/s00299-008-0557-2 ; ThakurA (2008), Micropropagation of Wild pear Pyrus pyrifoli ) Nakai II Induction of rooting, Notulae Botanicae Horti Agrobotanici Cluj - Napoca, 36, 104. ; LoSchiavoF (1989), DNA methylation of embryogenic carrot cell cultures and variations as caused by mutation differentiation hormones and hypomethylating drugs, Theoretical and Applied Genetics, 77, 325, doi.org/10.1007/BF00305823 ; HazarikaBN (2000), Sucrose - induced biochemical changes inin vitromicro shoots ofCitrusspecies, Indian Journal of Horticulture, 57, 27. ; LopezCMR (2010), Progressive erosion of genetic and epigenetic variation in callus - derived cocoa ( Theobroma cacao ) plants, New Phytologist, 186, 856, doi.org/10.1111/j.1469-8137.2010.03242.x ; SunQ (2008), Rice jmjC domain - containing gene JMJ encodes demethylase required for floral organ development Proceeding of National Academy of, Science USA, 105, 9. ; LeeN (1988), Quantum flux density effects on the anatomy and surface morphology ofin vitroandin vivo - developed sweetgum leaves for, Journal of American Society Horticultural Science, 113, 167. ; DeKlerkGJ (2007), Stress in plants culturedin vitro, Propagation of Ornamental Plants, 7, 129. ; SinghaS (1990), Relationship between calcium and agar on vitrification and shoot tip necrosis of quince ( Cydonia oblongaMill ) shootin vitro, Plant Cell Tissue and Organ Culture, 23, 135, doi.org/10.1007/BF00035834 ; BrainerdKE (1982), Stomatal functioning ofin vitroand greenhouse apple leaves in darkness mannitol and CO, Journal of Experimental Botany, 33, 388, doi.org/10.1093/jxb/33.3.388 ; FurutaK (2011), The CKH / PKL chromatin remodeling factor negatively regulates cytokinin responses inArabidopsiscalli, Plant Cell Physiology, 52, 618, doi.org/10.1093/pcp/pcr022 ; IlievI (2011), Origin , morphology and anatomy of fasciation in plants culturedin vivoandin vitro, Plant Growth Regulation, 63, 115, doi.org/10.1007/s10725-010-9540-3 ; JoyceSM (2003), Stress and aberrant phenotypes inin vitroculture, Plant Cell Tissue and Organ Culture, 74, 103, doi.org/10.1023/A:1023911927116 ; BairuMW (2011), a Physiological and developmental problems encountered byin vitrocultured plants, Plant Growth Regulation, 63, 101, doi.org/10.1007/s10725-011-9565-2 ; HaoY (2003), Genetically stable regeneration of apple plants from slow growth, Plant Cell Tissue and Organ Culture, 72, 253, doi.org/10.1023/a:1022388728497 ; DeKlerkGJ (2011), Effects of phenolic compounds on adventitious root formation and oxidative decarboxylation of applied IAA inMalus Jork, Plant Growth Regulation, 9, 175, doi.org/10.1007/s10725-010-9555-9 ; SelvapandiyanA (1988), A simple method for direct transplantation of cultured plants to the field, Plant Science, 56, 81, doi.org/10.1016/0168-9452(88)90189-6 ; MathurA (2008), Biological hardening and genetic fidelity testing of the micro - cloned progeny ofChlorophytum borivilianum, African Journal of Biotechnology, 7, 1046. ; GroutBWW (1978), b Transplanting of the cauliflower plants regenerated from meristem culture II CO fixation and the development of photosynthetic ability, Horticulture Research, 17, 65. ; MachadoMP (2014), Influence of calcium content of tissue on hyperhydricity and shoot tip necrosis ofin vitroregenerated shoots ofLavandula angustifoliaMill, Brazilian Archives of Biology and Technology, 57, 636, doi.org/10.1590/S1516-8913201402165 ; FabbriA (1986), Anatomical changes in persistent leaves of the tissue - cultured strawberry plant after the removal from culture, Scientia Horticulturae, 28, 331, doi.org/10.1016/0304-4238(86)90107-X ; ApterRC (1993), b In vitroandex vitroadventitious root formation in Asian Jasmine ( Trachelospermum asiaticum ) I : comparative morphology for, Journal of American Society Horticultural Science, 118, 902. ; IlievI (2003), Anatomical study ofin vitroobtained fasciated shoots fromBetula pendula Roth, Acta Horticulture, 616, 481, doi.org/10.17660/ActaHortic.2003.616.76 ; KitinP (2005), A comparative histological study between normal and fasciated shoots ofPrunus aviumgeneratedin vitro, Plant Cell Tissue and Organ Culture, 82, 141, doi.org/10.1007/s11240-004-8153-9 ; AbousalimA (1994), A practical method for alleviating shoot - tip necrosis symptom in thein vitroshoot culture ofPistiacia veracv Mateur, Journal of Horticultural Science, 69, 357. ; BadrA (2007), Sugar uptake and metabolism in tissue cultured potato plantlets cultured in liquid medium, Acta Horticulturae, 748, 265, doi.org/10.17660/ActaHortic.2007.748.36 ; LaufsP (1998), b Cellular parameters of the shoot apical meristem inArabidopsis, Plant Cell, 10, 1375, doi.org/10.1105/tpc.10.8.1375 ; ChakrabartyD (2003), Detection of DNA methylation changes during somatic embryogenesis of siberian ginseng, Plant Science, 165, 61, doi.org/10.1016/S0168-9452(03)00127-4 ; BrainerdKE (1981), Leaf anatomy and water stress of aseptically cultured pixy plum grown under different environments, HortScience, 16, 173. ; GesteiraAS (2002), RAPD - based detection of genomic instability in soybean plants derived from somatic embryogenesis, Plant Breeding, 121, 269, doi.org/10.1046/j.1439-0523.2002.00708.x ; YokotaS (2007), Histological observations of changes in leaf structure during successive micropropagation stages inAralia elataandPhellodendron amurense, Plant Biotechnology, 24, 221, doi.org/10.5511/plantbiotechnology.24.221 ; BairuMW (2011), b Somaclonal variation in plants : causes and detection methods, Plant Growth Regulation, 63, 147, doi.org/10.1007/s10725-010-9554-x ; JiangC (2011), RegenerantArabidopsislineages display distinct genome - wide spectrum of mutations conferring variant phenotypes, Current Biology, 21, 1385, doi.org/10.1016/j.cub.2011.07.002 ; WardleK (1983), In vitroacclimatization of aseptically - cultured plantlets to humidity for, Journal of American Society Horticultural Science, 108, 386. ; FrankT (2004), Hyperhydricity ofPrunus aviumshoots cultured on gelrite : a controlled stress response, Plant Physiology and Biochemistry, 42, 519, doi.org/10.1016/j.plaphy.2004.05.003 ; FukaiS (2000), Morphological characteristics and shoot regeneration potential of the fasciated rhizome ofCymbidium kanranMakino, Plant Biotechnology, 17, 259, doi.org/10.5511/plantbiotechnology.17.259 ; Buddendorf (1995), Controlling the gaseous composition in thein vitrodescription of flow system and effects of the different gaseous component onin vitrogrowth of potato plantlets, Scientia Horticulturae, 65, 11, doi.org/10.1016/0304-4238(95)00857-8 ; ChiruvellaKK (2014), Phenotypic aberrations during micro - propagation ofSoymida febrifuga ( Roxb ) Adr Juss, Notulae Scientia Biologicae, 6, 99. ; BenniciA (2004), Genetic stability and uniformity ofFoeniculum vulgareMill regenerated plants through organogenesis and somatic embryogenesis, Plant Science, 166, 221, doi.org/10.1016/j.plantsci.2003.09.010 ; SkoogF (1957), Chemical regulation of growth and organ formation in plants tissue culturedin vitro Symposium of the Society for, Experimental Biology, 11, 118. ; SallanonH (1991), Ultrastructure and functioning of guard cells ofin vitrocultured rose plants, Plant Physiology and Biochemistry, 29, 333. ; PischkeMS (2006), A transcriptome - based characterization of habituation in plant tissue culture, Plant Physiology, 140, 1255, doi.org/10.1104/pp.105.076059 ; GroutBWW (1988), Photosynthesis of regenerated plantletsin vitroand the stress of transplanting, Acta Horticulturae, 230, 129, doi.org/10.17660/ActaHortic.1988.230.13 ; KennedyD (2005), What don t we know, Science, 309, 75, doi.org/10.1126/science.309.5731.75 ; GroutBWW (1978), a Modified leaf anatomy of the cauliflower plantlets regenerated from meristem culture, Annals of Botany, 42, 993. ; KonowEA (2001), Irradiance levels affectin vitroand greenhouse growth flowering and photosynthetic behavior of a hybridPhalaenopsisorchid for, Journal of American Society Horticultural Science, 126, 531. ; ViningK (2013), Methylome reorganization duringin vitrodedifferentiation and regeneration ofPopulus trichocarpa, BMC Plant Biology, 13. ; ZobayedSMA (2001), Micropropagation of potato ; evaluation of closed diffusive and forced ventilation on growth and tuberization, Annals of Botany, 87, 53, doi.org/10.1006/anbo.2000.1299

DOI

10.1515/abcsb-2015-0026

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