Tytuł artykułu

Anethum graveolens L. In Vitro Cultures – a Potential Source of Bioactive Metabolites, Phenolic Acids and Furanocoumarins

Tytuł czasopisma

Acta Biologica Cracoviensia s. Botanica




No 2

Autorzy publikacji

Wydział PAN

Nauki Biologiczne i Rolnicze


<jats:title>Abstract</jats:title> <jats:p>In vitro cultures of <jats:italic>Anethum graveolens</jats:italic> (dill) were maintained on the Linsmaier and Skoog (LS) medium – three variants, and the Murashige and Skoog (MS) medium – seven variants, which contained different amounts of plant growth regulators, cytokinin (BAP) and auxin (NAA) (from 0.1 mg l<jats:sup>−1</jats:sup> to 3.0 mg l<jats:sup>−1</jats:sup>). Methanolic extracts from in vitro grown biomass were analyzed by HPLC for free phenolic acids and furanocoumarins. The total amounts of free phenolic acids on the LS medium variants were similar (35.23–38.65 mg 100 g<jats:sup>−1</jats:sup> DW), but higher on the MS variants, ranging from about 66 mg 100 g<jats:sup>−1</jats:sup> DW to 100 mg 100 g<jats:sup>−1</jats:sup> DW. The main metabolites were: p-hydroxybenzoic acid (max. 24.41 mg 100 g<jats:sup>−1</jats:sup> DW) on the LS<jats:sup>−</jats:sup>based media, and salicylic acid (max. 57.88 mg 100 g<jats:sup>−1</jats:sup> DW) and p-hydroxybenzoic acid (max. 36.27 mg 100 g<jats:sup>−1</jats:sup> DW) on the MS<jats:sup>−</jats:sup>based media. The total amounts of furanocoumarins were lower, as they did not exceed 8.5 mg 100 g<jats:sup>−1</jats:sup> DW on the LS media and 25 mg 100 g<jats:sup>−1</jats:sup> DW on the MS media. The main compounds in this group were bergapten (max. 15.01 mg 100 g<jats:sup>−1</jats:sup> DW) and marmesin (max. 8.12 mg 100 g<jats:sup>−1</jats:sup> DW). The MS variant containing 0.5 mg l<jats:sup>−1</jats:sup> BAP and 2.0 mg l<jats:sup>−1</jats:sup> NAA was proposed as the best production medium for both groups of metabolites. The maximum total amounts of free phenolic acids obtained in the in vitro grown biomass were slightly higher than their amounts in the fruits of the mother plant analyzed for comparison (99.66 mg 100 g<jats:sup>−1</jats:sup> DW and 93.34 mg 100 g<jats:sup>−1</jats:sup> DW, respectively); the maximum total amounts of furanocoumarins were approximately 1.8 times higher than in the fruits (24.26 mg 100 g<jats:sup>−1</jats:sup> DW and 13.67 mg 100 g<jats:sup>−1</jats:sup> DW, respectively).</jats:p>


Biological Commission of the Polish Academy of Sciences – Cracow Branch


2015[2015.01.01 AD - 2015.12.31 AD]


ISSN 0001-5296 ; eISSN 1898-0295


ShyuYS (2009), andYangDJ Evaluation of antioxidant ability of ethanolic extract from dill ( Anethum graveolensL ) flower, Food Chemistry, 115, 515, ; EkiertH (2014), andMuszyńskaB Accumulation of p - coumaric acid and other bioactive phenolic acids in in vitro culture ofRuta graveolensssp divaricata Gams, Medicina Internacia Revuo, 102, 25. ; Sidwa (2003), andŁojkowskaE Establishment of a co - culture ofAmmi majusL andRuta graveolensL for the synthesis of furanocoumarins, Plant Science, 165, 1315, ; LeeY (2011), andKimMW Influence of auxins cytokinins and nitrogen on production of rutin from callus and adventitious roots of the white mulberry tree ( Morus albaL, Plant Cell Tissue and Organ Culture, 105, 9, ; SzopaA (2011), andEkiertH Lignans inSchisandra chinensisin vitro cultures, Pharmazie, 66, 633. ; CallanNW (2007), andWeltyLE Herb and oil composition of dill ( Anethum graveolensL Effects of crop maturity and plant density, Industrial Crops and Products, 25, 282, ; SzopaA (2014), andEkiertH Production of biologically active phenolic acids inAronia melanocarpa in vitro cultures cultivated on different variants of the Murashige and Skoog medium, Plant Growth Regulation, 72, 51, ; FariaJms (2009), andBarrosoJG Biotransformation of menthol and geraniol by hairy root cultures ofAnethum graveolens : effect on growth and volatile components, Biotechnology Letters, 31, 897, ; EkiertH (2000), andGomöłkaE a Coumarin compounds inAmmi majusL callus cultures, Pharmazie, 55, 684. ; KamleshD (2008), andKakasahebRM Efficient and Sensitive Method for Quantitative Determination and Validation of Umbelliferone Carvone and Myristicin inAnethum graveolensandCarum carviSeed, Chromatographia, 67, 163, ; EkiertH (2000), andGomołkaE b Furanocoumarins inPastinaca sativaL in vitro culture, Pharmazie, 55, 618. ; JanaS (2011), andShekhawatGS Plant growth regulators adenine sulfate and carbohydrates regulate organogenesis and in vitro flowering ofAnethum graveolens, Acta Physiologiae Plantarum, 33, 305, ; YiliA (2006), andSalikhovSHI Isolation of biocidal peptides fromAnethum graveolensseeds, Chemistry of Natural Compounds, 42, 588, ; JanaS (2012), andShekhawatGS In vitro regeneration ofAnethum graveolens antioxidative enzymes during organogenesis and RAPD analysis for clonal fidelity, Biologia Plantarum, 56, 9, ; SzopaA (2012), andEkiertH Studies on the accumulation of free phenolic acids in in vitro cultures ofSchisandra chinensis Chinese magnolia vine ), Applied Biochemistry and Biotechnology, 166, 1941. ; EkiertH (2013), SzopaA Rosmarinic acid production in plant in vitro cultures of, Polish Journal Cosmetology, 16, 49. ; SzopaA (2012), andFugasE Production of bioactive phenolic acids and furanocoumarins in in vitro cultures ofRuta graveolensL andRuta graveolensssp divaricata Gams under different light conditions and, Plant Cell Tissue Organ Culture, 110, 329, ; TianS (2005), andKayaharaH High - performance liquid chromatographic determination of phenolic compounds in rice, Journal of Chromatography A, 1063. ; EkiertH (2007), andCzyganFC Secondary metabolites in in vitro cultures ofRuta graveolensL andRuta graveolensssp divaricata Gams In : Ramawat KG eds Biotechnology : secondary metabolites Plants and microbes Publishers Plymouth, Science, 445. ; MurashigeT (1962), andSkoogF A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiologia Plantarum, 15, 473, ; GroverA (2012), andSundarD Production of monoterpenoids and aroma compounds from cell suspension cultures ofCamellia sinensis and, Plant Cell Tissue Organ Culture, 108, 323, ; ElTahchyA (2011), andLaurain - MattarD Effects of sucrose and plant growth regulators on acetylcholinesterase inhibitory activity of alkaloids accumulated in shoot cultures of Amaryllidaceae and, Plant Cell Tissue Organ Culture, 106, 381, ; EkiertH (2009), andKuśA Free phenolic acids inRuta graveolensL in vitro culture, Pharmazie, 64, 694. ; OrlitaA (2008), andStepnowskiP b Application of chitin and chitosan as elicitors of coumarins and furoquinolone alkaloids inRuta graveolensL ( common rue ), Biotechnology and Applied Biochemistry, 51, 91, ; SantosPAG (2002), andSchefferJJC Hairy root cultures ofAnethum graveolens ( dill establishment growth time - course study of their essential oil and its comparison with parent plant oils, Biotechnology Letters, 24, 1031, ; VanisreeM (2004), andTsayHS Plant cell cultures an alternative and efficient source for the production of biologically important secondary metabolites InternationalJournal of Applied and, Science Engineering Research, 2, 29. ; EkiertH (2000), Medicinal plant biotechnology : the Apiaceae family as the example of rapid development, Pharmazie, 55, 561. ; WenA (2003), andToivonenP Antilisterial activity of selected phenolic acids, Food Microbiology, 20, 305, ; RamawatKG (2007), andMathurM Factors affecting the production of secondary metabolites In : Ramawat KG and Merillon eds ] Biotechnology : secondary metabolites Plants and microbes Publishers Plymouth, Science, 59. ; SzopaA (2015), andEkiertH Production of schisantherin A and gomisin G in in vitro cultures ofSchisandra chinensis, Phytochemistry Letters, 11, 440, ; TirilliniB (1998), andRicciA Furocoumarin production by callus tissues ofHeracleum sphondyliumL, Phytotherapy Research, 12, 25,<S25::AID-PTR239>3.0.CO;2-N ; SzopaA (2013), andEkiertH Production of deoxyschizandrin and γ - schizandrin in shoot - differentiating and undifferentiating callus cultures ofSchisandra chinensis ( Chinese magnolia vine ), Journal of Biotechnology, 165. ; EkiertH (1999), andGomöłkaE Effect of light on contents of coumarin compounds in shoots ofRuta graveolensL cultured in vitro, Acta Societatis Botanicorum Poloniae, 68, 197, ; LinsmaierEM (1965), andSkoogF Organic growth factor requirements of tabacco tissue culture, Physiologia Plantarum, 18, 100, ; OrlitaA (2008), andStepnowskiP a Identification ofRuta graveolensL metabolites accumulated in the presence of abiotic elicitors, Biotechnology Progress, 24, 128, ; SzopaA (2013), andMuszyńskaB Accumulation of hydroxybenzoic acids and other biologically active phenolic acids in shoot and callus cultures ofAronia melanocarpa black chokeberry ) and, Plant Cell Tissue Organ Culture, 113, 323, ; BohuslavizkiKH (1994), andSanmannK Mode of action of psoralens acridinons and coumarins on the ionic currents in intact myelinated nerve fibres and its significance in demyelinating diseases, General Physiology and Biophysics, 13, 309. ; MakriO (2004), andKintziosS In vitro rosmarinic acid production : an update In ed Biotechnology of medicinal plants Vitalizer and therapeutic Publishers Plymouth, Science, 19.