Details

Title

Qa-SNARE Protein SYP22 Negatively Regulates Brassinosteroid Signaling in the Dark

Journal title

Acta Biologica Cracoviensia s. Botanica

Yearbook

2015

Numer

No 2

Publication authors

Divisions of PAS

Nauki Biologiczne i Rolnicze

Abstract

Abstract Soluble N-ethyl-maleimide sensitive factor attachment adaptor protein receptor (SNARE) domain-containing proteins were mainly involved in vesicle-associated membrane fusion. Genetic screening has revealed the function of SNARE in different aspects of plant biology. Among them, Synthaxin-22 (SYP22) a Qa-SNARE has been reported to have a pleiotropic function in plant development including regulation of leaf waving, shoot gravitropism and flowering time. In this study, we identified a new role of SYP22 in regulation of brassinosteroid (BR) signaling, especially in the dark. SYP22 interacts with BR receptor, brassinosteroid insensitive 1 (BRI1), and overexpression of SYP22 enhanced a weak BRI1 mutant bri1-5 phenotype. syp22 mutant exhibits short hypocotyl and it is sensitive to exogenously treated BR while slightly insensitive to BR-biosynthesis inhibitor propiconazole (PCZ) in the dark. Expression levels of BR signaling maker genes ACS5, SAUR15 and IAA19 were slightly higher, while BR6OX2, a BR biosynthesis marker gene, was lower in syp22 compared to the wild-type. In addition, syp22 was sensitive to 2,4-D, a synthetic auxin, in the dark. In conclusion, SYP22 is involved in BR- and auxin-mediated hypocotyl growth inhibition in the dark, which might be via interaction with BR and auxin key regulators to alter their internalization in Arabidopsis.

Publisher

Biological Commission of the Polish Academy of Sciences – Cracow Branch

Date

2015[2015.01.01 AD - 2015.12.31 AD]

Identifier

ISSN 0001-5296 ; eISSN 1898-0295

References

UemuraT (2004), Systematic analysis of SNARE molecules inArabidopsis : dissection of the post - Golgi network in plant cells Structure and, Cell Function, 29, 49, doi.org/10.1247/csf.29.49 ; ZhouX (2013), Brassinosteroids regulate the differential growth ofArabidopsishypocotyls through auxin signaling componentsiaa andarf, Molecular Plant, 6, 887, doi.org/10.1093/mp/sss123 ; JonesAM (2014), Border control - a membrane - linked interactome ofArabidopsis, Science, 344. ; ClouseSD (1998), BRASSINOSTEROIDS : Essential regulators of plant growth and development, Annual Review of Plant Physiology and Plant Molecular Biology, 49, 427, doi.org/10.1146/annurev.arplant.49.1.427 ; HamajiK (2009), Dynamic aspects of ion accumulation by vesicle traffic under salt stress inArabidopsis and, Plant Cell Physiology, 50, 2023, doi.org/10.1093/pcp/pcp143 ; ZhuZX (2014), Overexpression of a SNARE protein AtBS b alters BR response inArabidopsis, Botanical Studies, 55, 14. ; WangZY (2012), Brassinosteroid signaling network and regulation of photomorphogenesis, Annual Review of Genetics, 46, 701, doi.org/10.1146/annurev-genet-102209-163450 ; KimJG (2009), XanthomonasT S effector XopN suppresses PAMP - triggered immunity and interacts with a tomato atypical receptor - like kinase and TFT, Plant Cell, 21, 1305, doi.org/10.1105/tpc.108.063123 ; TaoLZ (2005), RAC GTPases in tobacco andArabidopsismediate auxin - induced formation of proteolytically active nuclear protein bodies that contain AUX / IAA proteins, Plant Cell, 17, 2369, doi.org/10.1105/tpc.105.032987 ; ShirakawaM (2009), Vacuolar SNAREs function in the formation of the leaf vascular network by regulating auxin distribution, Plant and Cell Physiology, 50, 1319, doi.org/10.1093/pcp/pcp076 ; SanderfootA (2007), Increases in the number of SNARE genes parallels the rise of multicellularity among the green plants, Plant Physiology, 144, 6, doi.org/10.1104/pp.106.092973 ; LiH (2001), The Rop GTPase switch controls multiple developmental processes inArabidopsis, Plant Physiology, 126, 670, doi.org/10.1104/pp.126.2.670 ; GeldnerN (2007), Endosomal signaling of plant steroid receptor kinaseBRI, Genes Development, 21, 1598, doi.org/10.1101/gad.1561307 ; EbineK (2012), Flowering time modulation by a vacuolar SNARE via FLOWERING LOCUS C inArabidopsis thaliana, Plos One, 7, 42239, doi.org/10.1371/journal.pone.0042239 ; OhnishiT (2012), a brassinosteroid biosynthetic cytochrome ofArabidopsis catalyzes oxidation of Biological Chemstry, Journal, 1. ; ChenLQ (2012), Sucrose efflux mediated by SWEET proteins as a key step for phloem transport, Science, 335. ; LipkaV (2007), SNARE - ware : the role of SNARE - domain proteins in plant biology of Developmental, Annual Review Cell Biology. ; BessererA (2012), Selective regulation of maize plasma membrane aquaporin trafficking and activity by the SNARE SYP, Plant Cell, 24, 121. ; LinD (2012), A ROP GTPase - dependent auxin signaling pathway regulates the subcellular distribution of PIN inArabidopsisroots, Current Biology, 2, 1319. ; OhtomoI (2005), Identification of an allele of VAM that confers a semi - dwarf pheno - type inArabidopsis thaliana and, Plant Cell Physiology, 22, 1358, doi.org/10.1093/pcp/pci146 ; EbineK (2008), A SNARE complex unique to seed plants is required for protein storage vacuole biogenesis and seed development ofArabidopsis thaliana, Plant Cell, 20, 3006, doi.org/10.1105/tpc.107.057711 ; LalondeS (2010), A membrane protein / signaling protein interaction network forArabidopsisversion AMPv in, Frontiers Physiology, 22, 1. ; OhE (2012), Interaction between BZR and integrates brassinosteroid and environmental responses, Nature Cell Biology, 4, 1. ; IraniNG (2012), Fluorescent castasterone revealsBRI signaling from the plasma membrane, Nature Chemical Biology, 8, 1. ; ChoeS (2001), Overexpression of DWARF in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield inArabidopsis, Plant Journal, 26, 573, doi.org/10.1046/j.1365-313x.2001.01055.x ; NibauC (2013), TheArabidopsissmall GTPase AtRAC is a modulator of auxin and abscisic acid signaling, Journal of Experimental Botany, 9, 3425, doi.org/10.1093/jxb/ert179 ; SutterJU (2006), Selective mobility and sensitivity to SNAREs is exhibited by theArabidopsisKAT channel at the plasma membrane, Plant Cell, 18, 1. ; Nole (2010), Synergistic disruptions in seuss cyp double mutants reveal a role for brassinolide synthesis during gynoecium and ovule development, BMC Plant Biology, 10, 85.

DOI

10.1515/abcsb-2015-0021

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