The study examined tyrosol glucosyltransferase activity and the efficiency of salidroside production in natural and transformed root cultures of Rhodiola kirilowii (Regel) Regel et Maximowicz. Neither enzyme activity nor salidroside accumulation were detected in natural and transformed root cultures maintained in media without tyrosol. To induce TGase activity in biotransformation reactions, tyrosol was added to natural and transformed root cultures on the day of inoculation. The first peak of TGase activity (0.23 U/μg) was detected on day 9 in natural root culture, accompanied by the highest salidroside content (15.79 mg/g d.w.), but TGase activity was highest (0.27 U/μg) on day 15. In transformed root culture, day 18 showed the highest TGase activity (0.15 U/μg), which coincided with the highest salidroside content (2.4 mg/g d.w.). Based on these results, tyrosol was added to the medium on the days of highest previously detected activity of TGase: day 15 for natural root cultures and day 18 for transformed root cultures. This strategy gave significantly higher yields of salidroside than in the cultures supplemented with tyrosol on the day of inoculation. In natural root culture, salidroside production reached 21.89 mg/g d.w., while precursor feeding in transformed root cultures caused a significant increase in salidroside accumulation to 7.55 mg/g d.w. In all treatments, salidroside production was lower in transformed than in natural root cultures.
Developing the empathic attitude is one of the tasks of medical education as it aff ects the quality of therapeutic contact in the relationship between the doctor and the patient, conditioning the treatment process. According to Davis’s concept, empathy is defi ned as an aff ective-cognitive reaction in the context of the other person’s experience. Aim: Analysis of profi les of empathic sensitivity in students of medicine. Group: Male and female students of the fi ft h year of medicine who agreed to participate in an anonymous study (n = 153; M = 57, F = 96; mean age: 23 years). Tools: Th e Empathetic Sensitivity Scale (EES), which is the Polish tool for Davis’s Interpersonal Reactivity Index (IRI) was used. Th e ESS includes three sub-scales: Empathic Care (EC), Personal Distress (PD) and Adopting Perspective (AP). Results: The raw results were converted into sten scores and for sten scores for all three dimensions of empathetic sensitivity no diff erences were found between male and female students. Th ree clusters (1: n = 33%, 2: n = 39%, 3: n = 28%), which diff er in terms of each distinguished indicator, were identifi ed. Conclusions: Th e first cluster characterizes empathetic people, both in the aff ective and cognitive spheres, and those dealing well with unpleasant emotions in situations diffi cult to others. Th e second cluster characterizes participants with the ability to recognize the needs of others and to take into account their perspectives; the third cluster includes participants with a tendency to focus on their own experiences emerging in response to other people’s suff ering but with the ability to understand a situation and show empathic concern for the other person. The most favourable profi le — for a future doctor as well as for his patients — is the fi rst cluster because the doctor, with his empathic sensitivity directed towards the other man, can deal with his own unpleasant emotions.