Optimum values of preload can be achieved in well-tried constructions and then applied in similar structures. For new structures, it is recommended to calculate the preload force and to test the correctness of calculation by means of experiments. In practice it may be necessary to introduce corrections, because not all real work parameters can be precisely known. Credibility of calculations depends, first of all, on the consistence between the assumptions concerning temperature conditions during work and elastic deformations of cooperating elements – first of all of a holder – and the real work conditions. The aim of the study is to determine how preload influences the work of a system of angular ball bearings, in relation to durability of bearing, moment of friction and rigidity of the bearing.