Titania nanotube (TNT) arrays fabricated by anodizing of titanium foil in organic (ethylene glycol) and inorganic (phosphoric acid) electrolytes and thermally modified in argon revealed much improved properties to detect hydrogen peroxide. Horseradish peroxidase and acetate thionine co-absorbed by a dip coating on the TNT electrode were used to detect hydrogen peroxide in phosphate buffered saline. The morphology and electrochemical properties of TNT arrays were studied by scanning electron microscopy, electrochemical impedance spectroscopy and cyclic voltammetry. Well defined oxidation and reduction peaks for potassium ferricyanide have been observed for TNT formed in ethylene glycol and annealed in argon. TNT arrays formed in organic electrolyte and annealed in argon indicated more favorable adsorption and electrochemical properties what was confirmed by detection of hydrogen peroxide towards analyte in phosphorate buffered saline solution.
In this study, we examined whether and to what extent oxidative stress is induced in seedlings of two winter triticale (Triticosecale Wittm.) varieties (susceptible Tornado and resistant Witon) in response to infestation by the cereal grain aphid (Sitobion avenae L.) and bird-cherry-oat aphid (Rhopalosiphum padi L.). We compared the level of hydrogen peroxide (H2O2) and lipid peroxidation products as well as markers of protein damage (protein-bound thiol and carbonyl groups). The studied parameters were measured at 6, 24, 48 and 96 h post-initial aphid infestation compared to the non-infested control seedlings. Our studies indicated that the cereal aphid feeding evoked oxidative stress in the triticale seedlings. Cereal aphid feeding increased the H2O2 level in triticale tissues, with maximum levels observed at 24 and 48 h post-infestation. Triticale infestation with aphids also increased lipid peroxidation products in triticale seedlings, with the maximal levels at 48 or 96 h post-infestation. Further, there was a reduction in protein thiol content and an increase in protein carbonyl content in the triticale seedlings after infestation with female aphids. Stronger triticale macromolecule damages were evoked by the oligophagous aphid R. padi. There was a more substantial protein thiol content reduction in the resistant Witon cultivar and higher accumulation of protein-bound carbonyls in the tissues of the susceptible Tornado cultivar. The changes were proportional to the aphid population and the time of aphid attack. These findings indicate that the defensive strategies against cereal aphid (S. avenae and R. padi) infestation were stimulated in triticale Tornado and Witon seedlings. Our results explain some aspects and broaden the current knowledge of regulatory mechanisms in plant-aphid interactions.
This paper proposes a new, simple and an efficient method for methanol formation under the cavitation influence of hydrogen-peroxide using a dynamic cavitation reactor. The process involves the reaction of the generated hydroxyl radical with propanebutane gas (C3–C4) to form propyl and butyl radicals which decompose into methyl radicals and alkenes, followed by the subsequent yield of methanol (via the interaction of methyl-radical with hydroxyl radical). Technological process parameters employed in this investigation are quite achievable for industrial production.
It is known that external diffusional resistances are significant in immobilized enzyme packed-bed reactors, especially at large scales. Thus, the external mass transfer effects were analyzed for hydrogen peroxide decomposition by immobilized Terminox Ultra catalase in a packed-bed bioreactor. For this purpose the apparent reaction rate constants, kP, were determined by conducting experimental works at different superficial velocities, U, and temperatures. To develop an external mass transfer model the correlation between the Colburn factor, JD, and the Reynolds number, Re, of the type JD = K Re(n-1) was assessed and related to the mass transfer coefficient, kmL. The values of K and n were calculated from the dependence (am kp-1 - kR-1) vs. Re-1 making use of the intrinsic reaction rate constants, kR, determined before. Based on statistical analysis it was found that the mass transfer correlation JD = 0.972 Re-0.368 predicts experimental data accurately. The proposed model would be useful for the design and optimization of industrial-scale reactors.
On the basis of hydrogen peroxide decomposition process occurring in the bioreactor with fixed-bed of commercial catalase the optimal feed temperature was determined. This feed temperature was obtained by maximizing the time-average substrate conversion under constant feed flow rate and temperature constraints. In calculations, convection-diffusion-reaction immobilized enzyme fixed-bed bioreactor described by a coupled mass and energy balances as well as general kinetic equation for rate of enzyme deactivation was taken into consideration. This model is based on kinetic, hydrodynamic and mass-transfer parameters estimated in earlier work. The simulation showed that in the biotransformation with thermal deactivation of catalase optimal feed temperature is only affected by kinetic parameters for enzyme deactivation and decreases with increasing value of activation energy for deactivation. When catalase undergoes parallel deactivation the optimal feed temperature is strongly dependent on hydrogen peroxide feed concentration, feed flow rate and diffusional resistances expressed by biocatalyst effectiveness factor. It has been shown that the more significant diffusional resistances and the higher hydrogen peroxide conversions, the higher the optimal feed temperature is expected.
Optimal feed temperature was determined for a non-isothermal fixed-bed reactor performing hydrogen peroxide decomposition by immobilized Terminox Ultra catalase. This feed temperature was obtained by maximizing the average substrate conversion under constant feed flow rate and temperature constraints. In calculations, convection-diffusion-reaction immobilized enzyme fixed-bed reactor described by a set of partial differential equations was taken into account. It was based on kinetic, hydrodynamic and mass transfer parameters previously obtained in the process of H2O2 decomposition. The simulation showed the optimal feed temperature to be strongly dependent on hydrogen peroxide concentration, feed flow rate and diffusional resistances expressed by biocatalyst effectiveness factor.