One of the actual challenges in tissue engineering applications is to efficiently produce as high of number of cells as it is only possible, in the shortest time. In static cultures, the production of animal cell biomass in integrated forms (i.e. aggregates, inoculated scaffolds) is limited due to inefficient diffusion of culture medium components observed in such non-mixed culture systems, especially in the case of cell-inoculated fiber-based dense 3D scaffolds, inside which the intensification of mass transfer is particularly important. The applicability of a prototyped, small-scale, continuously wave-induced agitated system for intensification of anchorage-dependent CP5 chondrocytes proliferation outside and inside three-dimensional poly(lactic acid) (PLA) scaffolds has been discussed. Fibrous PLA-based constructs have been inoculated with CP5 cells and then maintained in two independent incubation systems: (i) non-agitated conditions and (ii) culture with wave-induced agitation. Significantly higher values of the volumetric glucose consumption rate have been noted for the system with the wave-induced agitation. The advantage of the presented wave-induced agitation culture system has been confirmed by lower activity of lactate dehydrogenase (LDH) released from the cells in the samples of culture medium harvested from the agitated cultures, in contrast to rather high values of LDH activity measured for static conditions. Results of the proceeded experiments and their analysis clearly exhibited the feasibility of the culture system supported with continuously wave-induced agitation for robust proliferation of the CP5 chondrocytes on PLA-based structures. Aside from the practicability of the prototyped system, we believe that it could also be applied as a standard method offering advantages for all types of the daily routine laboratory-scale animal cell cultures utilizing various fiber-based biomaterials, with the use of only regular laboratory devices.
One of the crucial advancements in next-generation 5G wireless networks is the use of high-frequency signals specifically those are in the millimeter wave (mm-wave) bands. Using mmwave frequency will allow more bandwidth resulting higher user data rates in comparison to the currently available network. However, several challenges are emerging (such as fading, scattering, propagation loss etc.), whenever we utilize mm-wave frequency wave bands for signal propagation. Optimizing propagation parameters of the mm-wave channels system are much essential for implementing in the real-world scenario. To keep this in mind, this paper presents the potential abilities of high frequencies signals by characterizing the indoor small cell propagation channel for 28, 38, 60 and 73 GHz frequency band, which is considered as the ultimate frequency choice for many of the researchers. The most potential Close-In (CI) propagation model for mm-wave frequencies is used as a Large-scale path loss model. Results and outcomes directly affecting the user experience based on fairness index, average cell throughput, spectral efficiency, cell-edge user’s throughput and average user throughput. The statistical results proved that these mm-wave spectrum gives a sufficiently greater overall performance and are available for use in the next generation 5G mobile communication network.