The study was to determine the effect of water activity (0.850; 0.900; 0.950; 0.995; and 0.999 aw) on the growth of T. lanuginosus on solid media containing different cellulose substrates (crystalline cellulose, carboxymethyl cellulose - CMC, fi lter paper, and sawdust) and xylan. The growth of isolates from coffee beans and garden composts were compared. All isolates did not grow on media with aw < 0.950. On media with aw > 0.950, the hydrolysis zones were only observed on xylan and CMC. The highest daily growth and hydrolysis zone rates were mostly obtained at 0.995 aw and the lowest values were observed at 0.950 aw. The coffee beans isolates at 0.950 aw had the CMC hydrolysis coeffi cient 1.7-times higher than that for xylan. The fungal growth (FG) coeffi cient data indicate that the coffee beans isolates were able to utilize CMC and crystalline cellulose for growth and the highest growth rate was obtained at 0.999 aw. Subsequently, the compost isolates were able to grow on all substrates but the highest growth rate was obtained on CMC at 0.950 and 0.999 aw. Thus, coffee beans and composts provide T. lanuginosus isolates with various growth and hydrolytic zone rates in the range of 0.950−0.999 aw.
The quantitative description of an airlift bioreactor, in which aerobic biodegradation limited by carbonaceous substrate and oxygen dissolved in a liquid takes place, is presented. This process is described by the double-substrate kinetics. Mathematical models based on the assumption of plug flow and dispersion flow of liquid through the riser and the downcomer in the reactor were proposed. Calculations were performed for two representative hydrodynamic regimes of reactor operation, i.e. with the presence of gas bubbles only within the riser and for complete gas circulation. The analysis aimed at how the choice of a mathematical model of the process would enable detecting the theoretical occurrence of oxygen deficiency in the airlift reactor. It was demonstrated that the simplification of numerical calculations by assuming the “plug flow” model instead of dispersion with high Péclet numbers posed a risk of improper evaluation of the presence of oxygen deficiency zones. Conclusions related to apparatusmodelling and process design were drawn on the basis of the results obtained. The paper is a continuation of an earlier publication (Grzywacz, 2012a) where an analysis of single-substrate models of the airlift reactor was presented.