This communication reports detection of somaclonal variation among tissue culture-raised plants of Amorphophallus rivieri Durieu, an economically important crop in China, with high content of glucomannan in its corms. A population of regenerated plants was obtained from a single donor plant of A. rivieri via corm organogenesis, and 28 plants were randomly selected as a representative sample and subjected to analysis of somaclonal variation using inter-simple sequence repeat (ISSR) markers. Of the 26 ISSR primers screened, 13 gave distinct and reproducible band patterns, yielding 131 bands with an average of 10.1 bands per primer. Ten primers were polymorphic and generated 16 polymorphic bands with 12.2% mean polymorphism. Based on the ISSR data from the regenerated plants and the donor plant, Jaccard's similarity coefficients were calculated; they ranged from 0.961 to 1.000 with a mean of 0.982. A dendrogram was constructed using the unweighted pair group method with arithmetic mean (Upgma); it showed that a majority of regenerated plants (including the donor plant) clustered closely, with a mean similarity coefficient of 0.987. Low somaclonal variation observed in the regenerated plants indicates that rapid propagation of A. rivieri via corm organogenesis is a practicable method with a low risk of genetic instability.
The main optimized objects in underground mines include: stope layout, access layout and production scheduling. It is common to optimize each component sequentially, where optimal results from one phase are regarded as the input data for the next phase. Numerous methods have been developed and implemented to achieve the optimal solution for each component. In fact, the interaction between different phases is ignored in the tradition optimization models which only get the suboptimal solution compared to the integrated optimization model. This paper proposes a simultaneous integrated optimization model to optimize the three components at the same time. The model not only optimizes the mining layout to maximize the Net Present Value (NPV), but also considers the extension sequence of stope extraction and access excavation. The production capacity and ore quality requirement are also taken into account to keep the mining process stable in all mine life. The model is validated to a gold deposit in China. A two-dimensional block model is built to do the resource estimation due to the clear boundary of the hanging wall and footwall. The thickness and accumulation of each block is estimated by Ordinary Kriging (OK). In addition, the conditional simulation method is utilized to generate a series of orebodies with equal possibility. The optimal solution of optimization model is carried out on each simulated orebody to evaluate the influence of geological uncertainty on the optimal mining design and production scheduling. The risk of grade uncertainty is quantified by the possibility of obtaining the expected NPV. The results indicate that the optimization model has the ability to produce an optimal solution that has a good performance under the uncertainty of grade variability.
One of the most critical aspects of mine design is to determine the optimum cut-off grade. Despite Lane’s theory, which aims to optimize the cut-off grade by maximizing the net present value (NPV), which is now an accepted principle used in open pit planning studies, it is less developed and applied in optimizing the cut-off grade for underground polymetallic mines than open pit mines, as optimization in underground polymetallic mines is more difficult. Since there is a similar potential for optimization between open pit mines and underground mines, this paper extends the utilization of Lane’s theory and proposes an optimization model of the cut-off grade applied to combined mining-mineral processing in underground mines with multi-metals. With the help of 3D visualization model of deposits and using the equivalent factors, the objective function is expressed as one variable function of the cut-off grade. Then, the curves of increment in present value versus the cut-off grade concerning different constraints of production capacities are constructed respectively, and the reasonable cut-off grade corresponding to each constraint is calculated by using the golden section search method. The defined criterion for the global optimization of the cut-off grade is determined by maximizing the overall marginal economics. An underground polymetallic copper deposit in Tibet is taken as an example to validate the proposed model in the case study. The results show that the overall optimum equivalent cut-off grade, 0.28%, improves NPV by RMB 170.2 million in comparison with the cut-off grade policy currently used. Thus, the application of the optimization model is conducive to achieving more satisfactory economic benefits under the premise of the rational utilization of mineral resources.
Laplace Transform is often used in solving the free vibration problems of structural beams. In existing research, there are two types of simplified models of continuous beam placement. The first is to regard the continuous beam as a single-span beam, the middle bearing of which is replaced by the bearing reaction force; the second is to divide the continuous beam into several simply supported beams, with the bending moment of the continuous beam at the middle bearing considered as the external force. Research shows that the second simplified model is incorrect, and the frequency equation derived from the first simplified model contains multiple expressions which might not be equivalent to each other. This paper specifies the application method of Laplace Transform in solving the free vibration problems of continuous beams, having great significance in the proper use of the transform method.
In this study, medium-carbon steel was subjected to warm deformation experiments on a Gleeble 3500 thermosimulator machine at temperatures of 550°C and 650°C and strain rates of 0.001 s–1 to 1 s–1. The warm deformation behavior of martensite and the effects of strain rate on the microstructure of ultrafine grained medium-carbon steel were investigated. The precipitation behavior of Fe3C during deformation was analyzed and the results showed that recrystallization occurred at a low strain rate. The average ultrafine ferrite grains of 500 ± 58 nm were fabricated at 550°C and a strain rate of 0.001 s–1. In addition, the size of Fe3C particles in the ferrite grains did not show any apparent change, while that of the Fe3C particles at the grain boundaries was mainly affected by the deformation temperature. The size of Fe3C particles increased with the increasing deformation temperature, while the strain rate had no significant effect on Fe3C particles. Moreover, the grain size of recrystallized ferrite decreased with an increase in the strain rate. The effects of the strain rate on the grain size of recrystallized ferrite depended on the deformation temperature and the strain rate had a prominent effect on the grain size at 550°C deformation temperature. Finally, the deformation resistance apparently decreased at 550°C and strain rate of 1 s–1 due to the maximum adiabatic heating in the material.
In vitro embryogenic callus is a critical factor for genetic transformation of rice, especially for indica varieties. In this study, we investigated the relationship between polyamines, including putrescine (Put), spermidine (Spd) and spermine (Spm), and callus browning, and we studied the effect of exogenous Put on callus regeneration and on the content of endogenous polyamines. In addition, the expression levels of arginine decarboxylase gene (Adcl) and S-adenosylmethionine decarboxylase gene (Samdc) in embryogenic callus were studied by quantitative Real-time PCR analysis. The results showed that the contents of endogenous Put and Spd in the browning callus were significantly lower than those in normal callus. Exogenous Put could effectively improve the growing state of callus of indica rice and enhance the development of embryogenic callus. The content of endogenous polyamines in embryogenic callus, especially Spd and Spm, was increased after addition of exogenous Put. Additionally, exogenous Put also had an obvious impact on the expression levels of Adcl but partial effect on the expression levels of Samdc gene. This study could increase the knowledge of both embryogenic callus induction and polyamine catabolism in callus in indica rice.
The pool boiling characteristics of dilute dispersions of alumina, zirconia and silica nanoparticles in water were studied. These dispersions are known as nanofluids. Consistently with other nanofluid studies, it was found that a significant enhancement in Critical Heat Flux (CHF) can be achieved at modest nanoparticle concentrations (<0.1% by volume). Buildup of a porous layer of nanoparticles on the heater surface occurred during nucleate boiling. This layer significantly improves the surface wettability, as shown by a reduction of the static contact angle on the nanofluid-boiled surfaces compared with the pure-water-boiled surfaces. CHF theories support the nexus between CHF enhancement and surface wettability changes. This represents a first important step towards identification of a plausible mechanism for boiling CHF enhancement in nanofluids.
Objective: This study aimed to investigate developmental changes of the thymus and intra- thymic IL-1β, IL-6 and TNF-α expression in weaned Sprague-Dawley rats induced by lipopolysac- charide. Methods: Forty healthy weaned rats aged 26 days and weighing 83±4 g were randomly and equally divided into two groups. The lipopolysaccharide group was treated daily with a single injection of lipopolysaccharide for 10 consecutive days, and the saline group was treated with an equal volume of sterilized saline. On the 1st, 4th, 7th and 10th day, histological changes and distribu- tion of IL-1β-, IL-6- and TNF-α-positive cells were detected in the thymus by hematoxylin-eosin and immunohistochemistry staining, respectively. Subsequently, the expression levels of IL-1β, IL-6 and TNF-α were evaluated in the thymus by the ELISA method. Results: Thymus weight and index were significantly smaller in lipopolysaccharide-treated rats than in saline-treated rats (p<0.05), but no substantial changes were found in the thymus microstructure after lipopolysaccharide induction. Moreover, a large number of IL-1β-, IL-6- and TNF-α-positive cells were observed with brownish-yellow color and mainly distributed in the thy- mus parenchyma, both integrated optical density and average optical density increased signifi- cantly in lipopolysaccharide-treated rats than those in saline-treated rats. Compared with the saline group, most of the thymic homogenates had higher levels of IL-1β, IL-6 and TNF-α in the lipopolysaccharide group on different days. Conclusion: These findings indicate that the thymus atrophied after lipopolysaccharide induction in weaned Sprague-Dawley rats, and excessive production of intrathymic IL-1β, IL-6 and TNF-α was probably involved in the atrophic process.
In order to understand infection of avian influenza A virus (AIV) and canine distemper virus (CDV) in the Siberian Tiger in Northeast China, 75 Siberian Tiger serum samples from three cap- tive facilities in northeastern China were collected. AIV and CDV antibody surveillance was test- ed by using hemagglutination inhibition and serum neutralization methods. The results showed that the seroprevalence of H5 AIV, H9 AIV and CDV was respectively 9.33% (7/75), 61.33% (46/75) and 16% (12/75). In the 1<years <2 and > 5 year-old group, the seroprevalence of the H9 AIV was 24% and 80% (P < 0.01), and the CDV seroprevalence was 6% and 36% (P < 0.01), respectively. It was demonstrated that 3 (4%) out of 75 serum samples were AIV+CDV seropos- itive, with 2.67% (2/75) in H9+AIV and 1.33% (1/75) in H5+H9+AIV. To our knowledge, this is the first report of AIV and CDV seroprevalence in Siberian Tigers in China, which will provide base-line data for the control of AIV and CDV infection in Siberian Tigers in China.
In this study, non-sintered ceramsite was prepared using coal gasiﬁcation coarse slag obtained from a methanol plant. The basic performance and heavy metal leaching toxicity were analyzed. The results showed that seven out of nine non-sintered ceramsite groups were in accordance with the national standard of compressive strength (5 MPa), while only three groups met the national standard of water absorption index of less than 22%. The heavy metal concentrations in these three groups were found to be lower than that speciﬁed in National Class IV of surface water environment standards. The concentration of Cr was found to be 16.45 μg/L, which represents only 1% of the IV standard. The optimum mixing ratio, which showed high compressive strength (6.76 MPa) and low water absorption (20.12%), was found to be 73% coal gasiﬁcation coarse slag, 15% cement, and 12% quartz sand. The characterization using Fourier transform infrared spectroscopy showed that the formation of gelatin in ceramsite enhances the performance of the ceramsite base and increases the immobilization of heavy metal. The study proved that the preparation of non-sintered ceramsite using coal gasiﬁcation coarse slag reduces its environmental risk and achieves efﬁcient utilization of the slag. Therefore, it can be concluded that it is a feasible and environmental friendly method for the disposal of coal slag.
At present, with the increase of production capacity and the promotion of production, the reserves of most mining enterprises under the original industrial indexes are rapidly consumed, and the full use of low-grade resources is getting more and more attention. If mining enterprises want to make full use of low-grade resources simultaneously and obtain good economic benefits to strengthening the analysis and management of costs is necessary. For metal underground mines, with the gradual implementation of exploration and mining projects, capital investment and labor consumption are dynamic and increase cumulatively in stages. Consequently, in the evaluation of ore value, we should proceed from a series of processes such as: exploration, mining, processing and the smelting of geological resources, and then study the resources increment in different stages of production and the processing. To achieve a phased assessment of the ore value and fine evaluation of the cost, based on the value chain theory and referring to the modeling method of computer integrated manufacturing open system architecture (CIMOSA), the analysis framework of gold mining enterprise value chain is established based on the value chain theory from the three dimensions of value-added activities, value subjects and value carriers. A value chain model using ore flow as the carrying body is built based on Petri nets. With the CPN Tools emulation tool, the cycle simulation of the model is carry out by the colored Petri nets, which contain a hierarchical structure. Taking a large-scale gold mining enterprise as an example, the value chain model is quantified to simulate the ore value formation, flow, transmission and implementation process. By analyzing the results of the simulation, the ore value at different production stages is evaluated dynamically, and the cost is similarly analyzed in stages, which can improve mining enterprise cost management, promote the application of computer modeling and simulation technology in mine engineering, more accurately evaluate the economic feasibility of ore utilization, and provide the basis for the value evaluation and effective utilization of low-grade ores.
The uncontrolled rectifier and controlled rectifier which use fixed switching frequency control strategy are applied usually during the working of a high-power high- speed permanent magnet generator (HSPMG). Even for the controlled rectifier, it will generate harmonics. The electromagnetic performance of the HSPMG is also affected by these harmonics. In this paper, the influences of the fixed switching frequency control strategy on a HSPMG were studied. Based on the Fourier theory, the harmonic currents of the generator were analyzed, and the change of harmonic distribution range and current total harmonic distortion (THD) were obtained. By using an indirect field-circuit coupling method, the influences of the fixed switching frequency control strategy on the losses and torque of the generator were analyzed. The relations between the switching frequency and the losses and torque of the generator were obtained, and the change mechanism of the loss was revealed. The obtained conclusions can provide reference for the optimized choice of the switching frequency of the distributed generation system with the HSPMG. It can also provide support for the HSPMG electromagnetic structural optimization and the optimization of the loss and harmonic on the system level.