Animals as a source of organs and tissues for xenotransplantation could become a backup solution for the growing shortage of human donors. The presence of human xenoreactive anti- bodies directed against Galα1,3Gal antigens on the cell surface of a pig donor triggers the activa- tion of the complement leading to a hyperacute reaction. The development of genetic engineer- ing techniques has enabled the modification of genomes by knocking in and/or knocking out genes. In this paper, we report the generation of modified pigs with ZFN mediated disruption of the GGTA1 gene encoding the enzyme responsible for synthesis of Galα1,3Gal antigens. ZFN plasmids designed to target the exon 9 region of the pig GGTA1 gene encoding the catalytic domain were injected into the pronuclei of fertilized egg cells. Among 107 piglets of the F0 gene- ration analyzed, one female with 9-nt deletion in exon 9 of the GGTA1 gene was found. 13 of 33 piglets of the F1 generation represented the +/- GGTA1 genotype and 2 of 13 F2 piglets repre- sented the -/- GGTA1 genotype. No changes in the animals’ behavior, phenotype or karyotype were observed. Analysis confirmed heredity of the trait in all animals. A complex functional analysis of the modified animals, including flow cytometry, human serum cytotoxicity test and immunohistochemical detection, was performed to estimate the phenotype effect of genetic modification and this indicated an efficient GGTA1 knock-out in modified pigs.
Abstract The paper presents numerical analyses aimed at preliminary assessment of the protective panel effectiveness, which task is to protect the elements of building structures against explosion. For the criterion of assessing the effectiveness of the panel the load capacity of the column made of steel I-beam was chosen. Ultimate force was determined by using advanced computational procedure, which consisted of four stages: preload, blast simulation, dynamic response and static analysis of deformed structure. Blast load was simulated using Lagrangian- Eulerian domain coupling. Results indicated that the application of the protective panel significantly reduces the plastic deformation of the structure.
The results are based on two experimental high-manganese X98MnAlSiNbTi24-11 and X105MnAlSi24-11 steels subjected to thermo-mechanical treatment by hot-rolling on a semi-industrial processing line. The paper presents the results of diffraction and structural studies using scanning and transmission electron microscopy showing the role of Nb and Ti micro-additives in shaping high strength properties of high-manganese austenitic-ferritic steels with complex carbides. The performed investigations of two experimental steels allow to explain how the change cooling conditions after thermo-mechanical treatment of the analysed steels affects the change of their microstructure and mechanical properties. The obtained results allow assessing the impact of both the chemical composition and the applied thermo-mechanical treatment technology on the structural effects of strengthening of the newly developed steels.