The aim of the present work was to evaluate the selectivity of nicosulfuron, alone and in combinations, applied in post-emergence (V4) of glyphosate and sulfonylurea tolerant (RR/STS) soybean. The experiments were conducted in 2015/16 and 2016/17, in Piracicaba – state of São Paulo (SP). In 2016/17, the experiment was also conducted in Palotina – state of Paraná (PR). The experiment was a randomized block design, with four repetitions and 16 treatments, with combinations of nicosulfuron, glyphosate, chlorimuron, sulfometuron and cloransulam, applied alone or in tank mixture. Crop injury and variables related to agronomic performance were evaluated. Data were subjected to analysis of variance and treatment means were compared by the Tukey test. The results obtained are significant in the positioning of herbicides in RR/STS soybean, since in the five experiments, all the treatments were selective, except for glyphosate + sulfometuron which reduced the yield of a cultivar (CD 2630 RR/STS) in the 2015/16 season.